Feed for gene editing laboratory mice

Abstract

The invention discloses feed for gene editing laboratory mice. The feed is prepared from components in parts by weight as follows: 30-35 parts of corn, 10-15 parts of wheat, 15-20 parts of soybean meal, 5-12 parts of broken rice, 3-10 parts of fish meal, 1-5 parts of oil, 2-9 parts of a yeast hydrolysate, 0.8-1.2parts of stone powder, 1-2 parts of calcium hydrogen phosphate, 0.5-1 part of compound minerals, 0.1-0.5 parts of decavitamin, 0.1-0.2 parts of methionine and 0.1-0.2 parts of lysine. The feed has balanced nutrition and good palatability and can increase the feed intake of the gene editing laboratory mice, promote growth and breeding and significantly increase the survival rate of the gene editing laboratory mice, the nutrition index of the feed meets the nutrition index of national standard GB14924.3-2010 of Complete Nutritional Feed for Laboratory Animals, the hygienic index meets the hygienic index requirements of national standard GB14924.1-2010 of Complete Nutritional Feed for Laboratory Animals, and the feed for the gene editing laboratory mice has bright application prospects.

Description

technical field [0001] The invention belongs to the field of experimental animal nutrition, and more specifically relates to a feed for gene-edited experimental mice. Background technique [0002] Gene editing technology refers to the ability of humans to edit target genes to achieve knockout and addition of specific DNA fragments. There are three main implementation methods: (1) Gene knockout: If you want to lose the function of a certain gene, you can generate a double-strand DNA break (DSB) on this gene, which often occurs during the repair of non-homologous end joining. Insertion or deletion of DNA results in frameshift mutation, thereby realizing gene knockout; (2) Introduction of specific mutation: If you want to introduce a specific mutation into the genome, it needs to be achieved through homologous recombination. At this time, a Contains homologous templates for specific mutations. Under normal circumstances, the efficiency of homologous recombination is very low,...

AI Technical Summary

Problems solved by technology

[0004] Since the function of the immune system of the experimental mice after gene editing is significantly weaker than that of conventional experimental mice, they have the characteristics of slow growth and low reproductive ability. Feeding the gene-edited experimental mice with conventional experimental mouse feed will cause malnutrition and fertility Ability degradation and lead to the death of experimental mice, so it is necessary to develop special feed for gene-edited experimental mice

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