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Method for inducing chronic and acute liver failure mouse model by using carbon tetrachloride

A technology for acute liver failure and carbon tetrachloride, which is applied to preparations for in vivo experiments, pharmaceutical formulations, compound screening/testing, etc. The effect of short experiment period and easy operation

Inactive Publication Date: 2020-01-10
THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Application Information

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Problems solved by technology

The disadvantage of this model is that too many liver injury drugs are required for modeling, which will cause more interference factors for the follow-up research of the disease. Combined with the more common clinical HBV-related acute-on-chronic liver failure, most of them are induced by the independent factor of HBV virus. onset

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  • Method for inducing chronic and acute liver failure mouse model by using carbon tetrachloride
  • Method for inducing chronic and acute liver failure mouse model by using carbon tetrachloride

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Embodiment 1

[0029] A method for inducing acute-on-chronic liver failure mouse model with carbon tetrachloride, the steps of the method are as follows:

[0030] 1) Grouping method: 20 Balb / c inbred mice were randomly and equally divided into model group and control group;

[0031] 2) Administration method: dilute carbon tetrachloride with olive oil to a concentration of 10% and 50%, and continuously inject 10% CCl intraperitoneally at 5ml per kg body weight of the mice in the model group 4 , administered twice a week for 8 consecutive weeks; after 8 weeks, 50% CCl was administered intraperitoneally at 8 ml per kg body weight 4 ; mice in the control group were intraperitoneally injected with the same dose of olive oil at the same time;

[0032] 3) Detection method: Biochemical index detection and liver pathological examination were carried out on the experimental mice.

[0033] Liver pathological examination methods were as follows: Liver tissue samples were collected at 6h, 12h, 24h, and...

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Abstract

The invention relates to a method for inducing a chronic and acute liver failure mouse model, in particular to a method for inducing the chronic and acute liver failure mouse model by using carbon tetrachloride, which comprises the following steps of: a grouping method is characterized by randomly and averagely dividing Balb / c inbred line mice into a model group and a control group; an administration method is characterized by diluting carbon tetrachloride to concentrations of 10% and 50% by using olive oil, continuously injecting 10% carbon tetrachloride into the abdominal cavity of a mouse in the model group according to 5-8ml / kg body weight, administrating twice a week, and continuously administrating for 8 weeks; after 8 weeks, injecting the 50% carbon tetrachloride into the abdominalcavity once according to 5-8 ml / kg body weight; and a detection method is characterized by performing biochemical index detection and liver pathological examination on the experimental mouse. The method has the beneficial effects that Balb / c inbred line mice are adopted as model animals, most inbred lines can be adopted, the repeatability is better, the cost is lower compared with rats, and the operation is simpler and more convenient. The model better conforms to the disease development process of clinical hepatitis B virus (HBV) related chronic and acute liver failure.

Description

technical field [0001] The invention relates to a method for inducing acute-on-chronic liver failure mouse model, in particular to a method for inducing acute-on-chronic liver failure mouse model with carbon tetrachloride. Background technique [0002] Liver injury in animals is the result of various liver diseases, and the prevention and treatment of liver injury is still a serious issue. It is of great practical significance to establish experimental liver injury animal models to study the mechanism of liver disease, screen hepatoprotective drugs, and explore the principle of hepatoprotective action. [0003] Carbon tetrachloride (CCl 4 ) is widely used to induce liver injury in animal models, CCl 4 After the free radicals produced by metabolism enter the body, they are activated by cytochrome P450 in the liver to generate trichloromethyl free radicals and trichloromethyl peroxy free radicals, which attack the phospholipid molecules on the liver cell membranes, causing...

Claims

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Application Information

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IPC IPC(8): A61K31/02A61K49/00
CPCA61K31/02A61K9/0019A61K49/0008
Inventor 林炳亮熊静
Owner THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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