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Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1C3H/HeJ

A chromosome replacement and chromosome technology, applied in animal husbandry and other directions, can solve the problem of high infection rate of diarrhea

Inactive Publication Date: 2013-06-05
上海西普尔-必凯实验动物有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

③ The infection rate of diarrhea in young rats is high

Method used

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  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1C3H/HeJ
  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1C3H/HeJ
  • Construction for number 1 chromosome substitution laboratory mouse strain C57BL/6-Chr1C3H/HeJ

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Embodiment 1, the construction method of chromosome replacement mouse

[0104] Adult mice of two strains, C3H / HeJ and C57BL / 6, were crossed with C3H / HeJ as the female parent and C57BL / 6 as the male parent to obtain the F1 generation.

[0105] C57BL / 6 was obtained by crossing F1 generation male mice with C57BL / 6 female mice 1 -Chr1 C3H / HeJ , for backcross generation, in C57BL / 6 1 -Chr1 C3H / HeJ In male mice, the sex chromosomes and mitochondrial DNA in C3H / HeJ have been replaced by C57BL / 6; F2 generation male mice were crossed with C57BL / 6 female mice to obtain C57BL / 6 2 -Chr1C 3H / HeJ , in this way, ten generations of recurrent crosses were obtained, and ten generations of backcrossed mice C57BL / 6 were obtained. 10 -Chr1 C3H / HeJ , the schematic diagram of the construction process is as follows figure 1 .

Embodiment 2、1

[0106] Example 2, No. 1 chromosome genome identification

[0107] (1) Primary Screening and Identification of Chromosome 1 Genome

[0108] The gene sequences of chromosome 1 of the two strains of C3H / HeJ and C57BL / 6 were compared (refer to the public information of MGI (http: / / www.informatics.jax.org / ) for the sequence), and representative, two Different SNP sites of different strains, these SNP sites are evenly distributed on chromosome 1, and are easy to design and operate. Then design primers for these SNP sites to amplify sequences with characteristic SNP sites, and design the range of PCR amplified fragments between 100 and 400 bp. Primer design is mainly based on primer3 online software (http: / / frodo.wi .mit.edu / primer3 / ) and the Oligo6.0 program (Molecular Biology Insights Inc., USA) were completed. Site-specific probes and general probes were designed for these SNP sites for LDR, and the length of LDR probe ligation products was 80-130 bp.

[0109]The 12 SNP sites o...

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Abstract

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from a C3H / HeJ mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

Description

technical field [0001] The invention belongs to the field of mouse strain construction, in particular relates to the construction of a No. 1 chromosome replacement experimental mouse strain C57BL / 6-Chr1 C3H / HeJ . Background technique [0002] Since the end of the last century, mouse genetic resources have been widely used in the study of functional genes of diseases related to complex traits on a global scale. These diseases include: diabetes, atherosclerosis, heart disease, cancer, precocious puberty, glaucoma, hypertension, obesity , osteoporosis, mental illness, etc. More than 3,000 QTLs have been mapped so far, but only about 20 genes that regulate complex traits have been clearly identified. With the continuous deepening of research on genes related to complex traits, new strains of chromosome replacement have emerged. The construction of this resource has great research value and social benefits. So far, scientists from the United States, Japan, and the Czech Republ...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 赵莹赵丽亚刘磊邢正弘陈国强肖君华
Owner 上海西普尔-必凯实验动物有限公司
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