Real-time tracing irradiation device and method for experiment mice

Abstract

The invention discloses a real-time tracing irradiation device for experiment mice. The real-time tracing irradiation device for experiment mice comprises the following steps of S1, obtaining an imageof a mouse to be irradiated, with a video camera, judging the current posture of the mouse, and determining the region of the head of the mouse in the image; S2, obtaining the image of the mouse after the position of the mouse is moved, and according to the region of the head of the mouse determined in the step 1, determining the region of the head of the mouse in the new image by a target tracing algorithm; S3, calculating the pixel coordinates of the head of the mouse, amending the pixel coordinates of the head of the mouse according to the current posture of the mouse, and then calculatingdisplacement vectors needing ray movement according to the position relationship of the video camera in a practical device and a ray irradiation direction based on the pixel coordinates; and S4, controlling rays to perform corresponding displacement and irradiate the head of the mouse according to the displacement vector in the step S3. According to the real-time tracing irradiation device for experiment mice disclosed by the invention, amendment is performed on the position according to the posture of the mouse, the ray emitting position is adjusted in a real-time manner so that rays move along with the movement of the mouse, and the problem that the mouse moving region is limited is solved.

Description

technical field [0001] The invention relates to the intersecting fields of machinery, electronics and automation, in particular to an image positioning-based real-time tracking irradiation device and a method thereof. Background technique [0002] Optogenetics refers to a technology that combines optical and genetic means to activate or deactivate specific living tissues and cells. The theoretical approach is to incorporate opsin genes into brain neurons to create a tool to control neuronal excitation or inhibition with light. In optogenetics experiments, optical fibers are usually connected and fixed to the head of the mouse, so that light of a certain wavelength can be irradiated deep into the brain of the mouse. However, because the optical fiber hinders the movement of mice, this experimental method is not applicable in some studies on animal behavior. [0003] In 2017, some researchers proposed an experimental method of implanting a light-converting crystal into the h...

AI Technical Summary

Problems solved by technology

This method finds the position of the mouse head through image processing, corrects the position according to the posture of the mouse, calculates the actual spatial coordinates of the mouse head, and adjusts the position of the light emission in real time so that the light moves with the movement of the mouse. The problem of restricted activity area of ​​mice

Images