Method for establishing iron-overload mouse model corresponding to thalassemia Pesaro risk classification

A risk classification and mouse model technology, applied in the biological field, can solve problems such as patient death, adverse reactions, transplant rejection or recurrence, and achieve the effects of ensuring dosage, avoiding gastrointestinal irritating damage, and avoiding poisoning death

Inactive Publication Date: 2015-07-01
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Excessive doses of busulfan and cyclophosphamide used in conditioning regimens can cause adverse reactions leading to patient death; insufficient doses can cause graft rejection or relapse

Method used

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  • Method for establishing iron-overload mouse model corresponding to thalassemia Pesaro risk classification
  • Method for establishing iron-overload mouse model corresponding to thalassemia Pesaro risk classification
  • Method for establishing iron-overload mouse model corresponding to thalassemia Pesaro risk classification

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The establishment of a class I iron overload mouse model corresponding to the Pesaro risk classification of thalassemia includes the following steps:

[0023] Take healthy C57BL / 6 mice, male, 8 weeks old, weighing 19-23 grams. They were reared at 23±2°C and relative humidity of 55±5%, and the light / darkness was set as a 12h / 12h day and night cycle. They were given regular feed every day, free to eat and drink, and changed the litter 2-3 times a week. Iron dextran (Fe content 100 mg / ml) was injected intraperitoneally, 1 g iron element / kg body weight, once a week for a total of 1 week. The experiment was repeated three times.

[0024] The liver enlargement degree of mice was quantitatively analyzed by liver index (ratio of liver weight to body weight) and liver weight increase ratio [(liver index of mice in each group-liver index of control group) / liver index of control group*100]. The results in Table 1 show that the liver weight of mice increased by 18.7% after admin...

Embodiment 2

[0029] The establishment of a level II iron overload mouse model corresponding to the Pesaro risk classification of thalassemia includes the following steps:

[0030] Take healthy C57BL / 6 mice, male, 8 weeks old, weighing 19-23 grams. They were reared at 23±2°C and relative humidity of 55±5%, and the light / darkness was set as a 12h / 12h day and night cycle. They were given regular feed every day, free to eat and drink, and changed the litter 2-3 times a week. Intraperitoneal injection of iron dextran (Fe content 100mg / ml), 1g iron element / kg body weight, 1 time / week, administration for 2-4 weeks. The experiment was repeated three times

[0031] The results in Table 1 show that the mouse liver weight increased by 50.7-81.9% after 2-4 weeks of administration of iron dextran, and the weight increase was greater than 50%. It can be judged that the mouse has reached the corresponding standard of human liver enlargement exceeding 2cm in the Pesaro risk classification at this time ...

Embodiment 3

[0036] The establishment of a grade III iron overload mouse model corresponding to the Pesaro risk classification of thalassemia includes the following steps:

[0037] Take healthy C57BL / 6 mice, male, 8 weeks old, weighing 19-23 grams. They were reared at 23±2°C and relative humidity of 55±5%, and the light / darkness was set as a 12h / 12h day and night cycle. They were given regular feed every day, free to eat and drink, and changed the litter 2-3 times a week. Intraperitoneal injection of iron dextran (Fe content 100mg / ml), 1g iron element / kg body weight, 1 time / week, administration for 5-8 weeks. The experiment was repeated three times

[0038] The results in Table 1 show that the mouse liver weight increased by 92.3-135.7% after 5-8 weeks of administration of iron dextran, and the weight increase was greater than 50%. It can be judged that the mouse has reached the corresponding human liver enlargement of more than 2cm in the Pesaro risk classification at this time. standard...

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Abstract

The invention discloses a method for establishing an iron-overload mouse model corresponding to thalassemia Pesaro risk classification. The method comprises the following steps: injecting an iron-containing preparation to the abdominal cavity of a laboratory mouse, wherein the dosage is 1g / kg of the mouse, injecting the iron once a week, wherein the iron comprises Fe<2+> and Fe<3+>; establishing I-grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for one week; establishing II grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for 2-4 weeks; establishing III-grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for 5-8 weeks. The administration interval of the invention is in accordance with the pathological process of the hepatomegaly and hepatic fibrosis induced by the iron overload caused by repeated transfusion of the thalassemia patient, simultaneously, the mouse acute chalybeate poisoning death caused by the large-dose iron administration in a short time or the overlong mouse molding time caused by overlong iron administration time can be avoided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing an iron overload mouse model corresponding to the Pesaro risk classification of thalassemia. Background technique [0002] Thalassemia (referred to as thalassemia, thalassemia) is a kind of globin peptide chain (α, β chain) synthesis disorder anemia, which belongs to autosomal genetic disease. Because the early cases all came from the Mediterranean region, it is called thalassemia or thalassemia. Later, it was gradually found that this disease is widely distributed in tropical and subtropical regions of the world. The provinces south of the Yangtze River in my country are also high-incidence areas of the disease, especially in Guangdong, Guangxi, and Hainan. According to WHO estimates, about 345 million people in the world are carriers of thalassemia, including 80-90 million β-thalassemia carriers. The birth rate of children with severe thalass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/721A61K31/7016A61K31/295
Inventor 李续娥石俊敏陶宇晨张琪玥刘启发
Owner SOUTH CHINA NORMAL UNIVERSITY
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