Method for preparing intestinal mucosa injury animal model and applications of model

Abstract

The invention relates to the fields of medical treatment evaluation and detection technology, and in particular relates to a method for preparing an intestinal mucosa injury animal model and applications of the prepared intestinal mucosa injury animal model. According to the scheme, the medicine L-MSO having good water solubility and realizing convenient and simple gavaging operation is adopted, the mouse intestinal mucosa injury is conveniently and stably induced, and meanwhile, the corresponding tissue pathological change and inflammatory factor change are caused. For the preparation method provided by the invention, the intestinal mucosa injury animal model is prepared from the metabolism injury direction obviously different from the molding direction of immune injury inducing in the prior art, particularly, the low-concentration L-MSO is adopted for preparing the common injured model, wherein the intestinal mucosa of the laboratory mouse has the mild-moderate injury, the high-concentration L-MSO is adopted for preparing the seriously injured model, wherein the intestinal mucosa of the laboratory mouse has the severe injury; the prepared intestinal mucosa injury model has the significant values for the research and practice of inflammatory bowel diseases.

Description

Technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to a method for preparing an animal model of intestinal mucosal injury and its use. Background technique [0002] Data show that inflammatory bowel disease (Inflammatory Bowel Diseases, IBD) is a chronic intestinal disease of unknown etiology, including Ulcerative Colitis (UC) and Crohn’s Diseases (CD). Studies have shown that IBD tends to occur in young adults, and it is often a pattern of attack-remission-relapse, which seriously affects the quality of life of patients. Among them, UC mainly occurs in the rectum and colon, while CD lesions are more common in the terminal ileum and adjacent colon. The main manifestations of the inflammatory bowel disease are diarrhea, abdominal pain, weight loss, and abdominal masses. Among them, UC is often accompanied by pus and blood in the stool, which is also a high risk factor for colon cancer; and CD often has fistula for...

AI Technical Summary

Problems solved by technology

Practice has shown that the former induction method is relatively simple, but the success rate of modeling is related to the experimental concentration of the drug, the culture environment, and the differences in the species of experimental animals; the latter has higher modeling costs and longer modeling time

However, at present, chemical inducers mainly induce inflammation, but there is no ideal model to explore the occurrence of intestinal mucosal injury and intestinal inflammation from the perspective of metabolism or mucosal stem cell injury repair

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