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Culture medium for armillaria mellea spore separation and culture, method and application

A technology for spore separation and Armillaria spores, which is applied in the field of Armillaria spore separation and culture, can solve the problems of reducing the success rate of separation or purification, and achieve the effects of improving production and economic benefits, strong vitality and strong resistance to stress.

Active Publication Date: 2016-03-23
三峡旅游职业技术学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other miscellaneous bacteria can also grow deep into this medium, thus reducing the success rate of isolation or purification

Method used

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  • Culture medium for armillaria mellea spore separation and culture, method and application
  • Culture medium for armillaria mellea spore separation and culture, method and application
  • Culture medium for armillaria mellea spore separation and culture, method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1 basal medium culture isolates and cultivates Armillaria armillaria species

[0048] A culture medium for the isolation and cultivation of Armillaria spores, characterized in that: the culture medium includes the following components in parts by weight:

[0049] 200 parts of potatoes, 20 parts of sucrose, 18 parts of agar, 1000 parts of water;

[0050] The medium component potato slices were boiled for 15 minutes and filtered, the filtrate, sucrose, and agar were dissolved in water, and packed into triangular flasks, sealed, sterilized, and cooled to obtain the basic medium;

[0051]Collect Armillaria spores from Armillaria fruiting bodies, pick Armillaria spores and inoculate them on the basic medium for cultivation, the cultivation temperature is 20° C., and the cultivation time is 10 days, and the inoculated bottles infected with miscellaneous bacteria are removed;

[0052] Complete the separation and cultivation of spores. For its upbringing, see figu...

Embodiment 2

[0056] Embodiment 2 The patent culture medium of the present invention isolates and cultivates Armillaria mellea species

[0057] A kind of culture medium that is used for armillaria spore isolation, cultivation, described culture medium comprises the following components by weight:

[0058] 200 parts of potatoes, 20 parts of sucrose, 20 parts of agar, 180 parts of solid substrate, 1000 parts of water; the solid substrate is wheat.

[0059] Adopting described substratum to carry out the separation and cultivation of Armillaria spores, the method comprises the following steps:

[0060] 1) Soak the solid substrate in water for 28 hours to obtain material 1;

[0061] 2) Boil potato slices as the medium component for 15 minutes and filter, dissolve the filtrate, sucrose, and agar in water, and divide them into Erlenmeyer flasks to obtain the basal medium;

[0062] 3) Add material 1 to the basic medium in step 2), seal, sterilize, and cool to obtain a medium for the isolation and...

Embodiment 3

[0067] A medium for separating and cultivating Armillaria spores, said medium comprising the following components by weight: 150 parts of potatoes, 15 parts of sucrose, 25 parts of agar, 200 parts of solid substrate, and 1000 parts of water;

[0068] The solid substrate is barley;

[0069] The medium is a slant medium.

[0070] Adopting described substratum to carry out the separation and cultivation of Armillaria spores, the method comprises the following steps:

[0071] 1) Soak the solid substrate in water for 25 hours to obtain material 1;

[0072] 2) Boil potato slices as the medium component for 15 minutes and filter, dissolve the filtrate, sucrose, and agar in water, and divide them into Erlenmeyer flasks to obtain the basal medium;

[0073] 3) Add material 1 to the basic medium in step 2), seal, sterilize, and cool to obtain a medium for the isolation and cultivation of Armillaria spores, which are placed on inclined planes and uprights;

[0074] 4) Collect Armillari...

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Abstract

The invention relates to the technical field of edible fungus cultivation, in particular to a culture medium for armillaria mellea spore separation and culture, a method and application. The culture medium comprises a basic culture medium, wherein the basic culture medium is prepared from 150-220 parts of potatoes, 15-25 parts of sucrose, 15-25 parts of agar, 1000 parts of water and 150-200 parts of a solid matrix. The solid matrix is prepared from cereals or beans or grains or their combination. The culture medium is an oblique-plane culture medium. The method adopting the culture medium to perform armillaria mellea spore separation and culture comprises the steps that first, the basic culture medium is made; the solid matrix is soaked in water and then is put into the basic culture medium; after disinfection, sterilization and cooling are performed, armillaria mellea spores are introduced to be cultured into strains. The culture medium is suitable for shoestring and tissue block separation and spore separation on the armillaria mellea matrix, enables spores to be bred into stock cultures at a time, the time from separation to purification is 25 to 30 days, no tube transfer is needed in the midway, the strains can serve as test tube mother strains and can also directly serve as the stock cultures for usage, and the separation time and a breeding cycle of the armillaria mellea strains are shortened.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method and application for the isolation and cultivation of Armillaria spores. Background technique [0002] Armillaria mellea (Armillariella mellea) is a kind of edible and medicinal fungus belonging to the kingdom of fungi, Basidiomycota, Phytomycetes, Agaricales, Mildraceae, and Armillaria genus. Armillaria armillaria has a close relationship and co-evolution with Gastrodia elata, Polyporus and other organisms in nature. In the cultivation of Gastrodia elata and Polyporus chinensis, the activity of Armillaria species is either good or bad, which directly affects or determines their yield and quality. [0003] There are five techniques and methods ranging from low-level to high-level, simple to complex, for the acquisition or isolation of Armillaria species. The first is the separation of fungus material, the second is the separation of bacterial cords, th...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 王绍柏
Owner 三峡旅游职业技术学院
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