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Quality control method of armillaria mellea concentrated liquid

A technology of concentrated liquid and Armillaria, applied in the field of medicine, can solve the problem that the concentrated liquid of Armillaria does not have a legal standard and the like

Inactive Publication Date: 2015-01-28
JIANGXI HUIREN PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] However, there is no legal standard for the use of Armillaria concentrated solution as a medicine

Method used

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  • Quality control method of armillaria mellea concentrated liquid
  • Quality control method of armillaria mellea concentrated liquid
  • Quality control method of armillaria mellea concentrated liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Observation of the characteristics of honey fungus concentrate, the steps are:

[0083] This product is brownish-yellow to brownish-brown clear liquid with slightly sour, bitter and sweet taste.

[0084] The steps for the identification of honey fungus concentrate are:

[0085] Identification 1: Take a few drops of this product on filter paper, spray with ninhydrin test solution, after drying, it will show blue-purple or purple-red spots.

[0086] Identification 2: Take about 1.0ml of this product, add 10ml of water, shake well, take 2ml, add a few drops of 5% α-naphthol in ethanol, shake well, add 1ml of concentrated sulfuric acid along the wall, and it will be purple at the junction of the two liquid levels ring.

[0087] Identification 3: Take about 15ml of this product, place it in a separatory funnel, add ether to extract twice, combine the extracts, and evaporate to dryness. Add 1ml of ethanol to the residue to dissolve it as the test solution. Take an appropriate amount...

Embodiment 2

[0098] The invention is used for the quality evaluation of honey fungus concentrated liquid. The method of the present invention is used to evaluate the quality of multiple batches of Armillaria concentrate, and the results are shown in Table 8:

[0099] Table 8 Test results of different batches of samples

[0100]

[0101]

Embodiment 3

[0103] By adopting the method of the invention, the concentrated solution of armillaria is vacuum concentrated (55±5°C) to a relative density of 1.25, and the volume is reduced by 7.1 times. Dilute 7 times before testing, and the result meets the requirements. The test results after reconstitution with different relative densities are shown in Table 9-10.

[0104] Table 9 Test results after reconstitution with different relative densities

[0105]

[0106] Table 10 Measurement results before and after sample concentration

[0107]

[0108]

[0109] According to the conversion relationship formula between relative density and dilution factor:

[0110] ρ dilute =(ρ concentrated ·V concentrated +ρ water ·V water ) / (V water +V concentrated )

[0111] Take 20ml as the dilution object and calculate according to the above formula. The results are shown in Table 11.

[0112] Table 11 Dilution multiples of different densities

[0113]

[0114] It can be seen from the results of differen...

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PUM

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Abstract

The invention relates to a quality control method of an armillaria mellea concentrated liquid. The method includes character inspection, chemical component identification, measurement of density, a pH value, insoluble substances in water, and a microbial limit, and measurement of a peptide content, and adopts a higher concentration state for storage and transportation. By re-check and verification, the method can evaluate quality of the armillaria mellea concentrated liquid from a plurality of aspects.

Description

Technical field: [0001] The invention belongs to the field of medicine, and specifically relates to a quality control method of honey fungus concentrated liquid. Background technique: [0002] The honey fungus concentrate is the artificial liquid fermentation culture liquid of the Tricholomaceae fungus Armillaria mellea (Vahl.ex Fr) Quel. The liquid is concentrated and filtered. In recent years, a large number of research experiments have proved that the mycelium and fermentation broth of Armillaria have pharmacological and clinical effects similar to those of Gastrodia (Guo Shunxing, et al. The chemical composition and application of Armillaria[J].Microbiology Bulletin, 1996.23(4):239.). Pharmacological studies have shown that Armillaria concentrate mainly has the effects of sedation and hypnosis, anti-convulsions, protection of cardiovascular and cerebrovascular, improving vertigo syndrome, and strengthening immunity. Armillaria concentrate is the main raw material in the Chi...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N1/38G01N21/31G01N21/78G01N30/02G01N30/90
Inventor 周美娟胡浩武杜育华袁桂平梅玲华耿炤姜伟
Owner JIANGXI HUIREN PHARMA CO LTD
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