Preparation method of bottled armillaria mellea strain

A technology of armillaria and strains, applied in botany equipment and methods, fertilization devices, fertilizer mixtures, etc., can solve problems such as slow production speed, strain pollution, and polluted tampons, and save time and speed of adding water Fast and conducive to the effect of factory mass production

Inactive Publication Date: 2012-02-08
湖北森源生态科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you add too much water, the water will overflow during sterilization and contaminate the sealing materials such as tampons, and the sealing materials such as tampons are easy to be infected with bacteria during cultivation, causing bacterial contamination
And this kind of production speed is slow, labor-intensive and time-consuming

Method used

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  • Preparation method of bottled armillaria mellea strain

Examples

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Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method for bottled armillaria bacterial classification, the steps are:

[0038] Dry mix 78% miscellaneous sawdust, 20% wheat bran, 1% gypsum powder, and 1% sugar, and put them into a 500ml brine bottle manually. Put the whole basket into the sterilizing turnover basket, immerse the whole basket in the water pool, press it with a baffle, let the pool water flow into the strain bottle naturally, and fill it with water naturally. After 30 minutes of watering, take out the turnover basket, and transport the whole basket into the sterilization stove. Put in water steam to heat up, after the temperature rises to 97°C, keep the temperature for 2 hours, stop heating, transport the whole basket out of the sterilization stove for cooling, after cooling below 85°C, seal the saline bottle with a cotton plug, put The mouth of the brine bottle is tightly plugged, and after sealing, the whole basket is transported into the sterilizing stove again, and the stove is heated...

Embodiment 2

[0040] A preparation method for bottled armillaria bacterial classification, the steps are:

[0041]Dry mix 80% miscellaneous sawdust, 15% wheat bran, 3% corn flour, 1% gypsum powder, and 1% sugar, and put them into a 500ml brine bottle manually. Put the full brine bottle into the sterilized turnover basket, immerse the whole basket in the pool, press it with a baffle, let the pool water flow into the strain bottle naturally, and irrigate naturally. After 50 minutes of irrigation, take out the turnover basket and transport the whole basket into Sterilization stove, put water steam into the stove to heat, after the temperature rises to 101°C, keep the temperature for 1.5 hours, then stop heating, transport the whole basket out of the sterilization stove for cooling, after cooling to below 85°C, use a plastic bottle stopper Seal the brine bottle, plug the mouth of the brine bottle tightly, and then transport the whole basket into the sterilizing stove again, put water steam into...

Embodiment 3

[0043] A preparation method for bottled armillaria bacterial classification, the steps are:

[0044] Dry mix 80% miscellaneous sawdust, 15% wheat bran, 3% corn flour, 1% gypsum powder, and 1% sugar, and put them into a 500ml brine bottle with a bottling machine, and fill the compost up to the mouth of the brine bottle , and then put the full brine bottle into the sterilizing turnover basket, immerse the whole basket in the pool, press it with a baffle, let the pool water flow into the strain bottle naturally, and fill it naturally. After 60 minutes of watering, take out the turnover basket and put The baskets are transported into the autoclave cabinet, and water vapor is put into the sterilization cabinet to heat up. After the temperature rises to 105°C, keep the temperature for 1 hour, then stop heating, and the whole basket is transported out of the autoclave cabinet for cooling, and then cooled to 85°C. After that, seal the brine bottle with a cotton plug, plug the mouth of...

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Abstract

The invention discloses a preparation method of a bottled armillaria mellea strain. The method comprises the following steps of: mixing: weighing culture materials according to a culture medium formula and mixing uniformly in dry state; B, bottling: filling the uniformly-mixed dry culture materials into strain bottles; C, basketing and filling water: putting the filled strain bottles into a disinfection transition basket, immersing the entire basket into a water pool, and filling water; D, pre-sterilizing: pre-sterilizing the water-filled strain bottles; E, discharging: discharging the entire basket of pre-sterilized strain bottles out of a sterilizing stove; F, sealing: plugging and sealing the strain bottles with common strain sealing articles such as bottle plugs, bottle caps, cotton plugs and kraft paper; G, secondary sterilizing: transporting the sealed strain bottles into the sterilization stove once again for normal-pressure or high-pressure sterilization; and H, cooling and inoculating, wherein the strain bottles are required to be kept in upright state in the entire production process. Due to the adoption of the method, the bottling speed during armillaria mellea strain production can be increased, the sealing articles are prevented from being polluted, strain microbial contamination is reduced, and factory production is facilitated.

Description

technical field [0001] The invention relates to the technical field of edible and medicinal fungal strains, and more specifically relates to a method for preparing bottled Armillaria strains, which is suitable for bottling and sterilizing small-caliber bottled Armillaria strains in the production process, and is widely applicable Application in the production of moisture-loving edible fungus strains such as Armillaria strains. Background technique [0002] Armillaria mellea is also known as hazel mushroom, Zhen mushroom, honey mushroom, oak mushroom, grass mushroom, rhizome mushroom, honey ring mushroom, oak mushroom. Belonging to Basidiomycetes, Agaricaceae, Agaricaceae, Armillaria genus, it is a famous food and medicine fungus, and it is an indispensable symbiotic bacterium of Gastrodia elata, and the help of Armillaria mellea is necessary for the cultivation of Gastrodia elata. [0003] Armillaria armillaria strains are pure mycelium and cords that are artificially culti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 焦海涛周伟杨先新张顺凯
Owner 湖北森源生态科技股份有限公司
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