Armillaria mellea YN01 (WT) and application thereof

A typical technology of Armillaria mellea, applied in the field of microorganisms, can solve the problems of unfavorable artificial cultivation of Gastrodia elata, unknown sources of commercial species, unfavorable Armillaria mellea resources, etc., and achieve the effects of increasing the yield of Gastrodia elata, shortening the cultivation period, and shortening the co-cultivation time

Active Publication Date: 2017-05-10
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] As we all know, Gastrodia elata in Zhaotong is well-known in the world. In the 1950s, the clonal propagation of Gastrodia elata began in Zhaotong area, and large-scale planting has been widely implemented now. From 2011 to 2014, the city of Zhaotong has completed a total of 240,900 mu of gastrodia elata planting, with an output of 74.55 million kilograms ; but the Gastrodia elata market is still in short supply, and the prospect of Gastrodia elata planting is broad; there are problems such as unknown sources of commercial species and confusing classification of Gastrodia elata symbiotic Armillaria, which is not conducive to the scientific and rational development and utilization of Armillaria elata resources. Artificial cultivation is also very unfavorable; Zhaotong Gastrodia elata symbiotic Lemonella is also blank in basic research

Method used

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  • Armillaria mellea YN01 (WT) and application thereof
  • Armillaria mellea YN01 (WT) and application thereof
  • Armillaria mellea YN01 (WT) and application thereof

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1: Collection, isolation and identification of Armillaria armillaria

[0041] 1. Collect wild Gastrodia elata from Dazhaizi Township, Buluqi, Zhaotong City, wash it clean, soak it in alcohol with a volume concentration of 75% in an ultra-clean workbench and sterilize it, take the symbiotic part of Gastrodia elata and Armillaria mellea, and inoculate it on the ampicillin-containing On PDA medium, cultivate in the dark at 18°C; during this period, transfer the hyphae with germinated mycelia to PDA medium without antibiotics and culture in the dark at 25°C for 2 weeks. If there are morphological differences, a second separation is performed. The bacterial cords of the obtained strains were detected, and if blue-green fluorescence could be emitted at 530nm, the isolated strains could be preliminarily determined to be Armillaria armillaria. Afterwards, molecular identification was carried out, DNA was extracted by CTAB method, rDNA-IGS sequence amplification was ...

Embodiment 2

[0078] Embodiment 2: Armillaria armillaria strain seed dressing cultivation experiment

[0079] (1) Preparation of primary strains: Put 200mL PDA liquid medium after removing agar into a 500mL Erlenmeyer flask, sterilize and cool for later use, and insert the 0.5cm tip of the strain into the liquid medium (each bottle is connected with Into the 3 segment cable), in the dark at 25 ° C, 180r / min shaker culture 10d.

[0080] (2) Preparation of cultivars: Add branches with a diameter of 1-2 cm in a 380mL culture bottle as fungus materials, and add nutrient solution to the 2 / 3 of the bottle according to the ratio of 20% starch and 2% glucose, and submerge the culture solution. The bacterial material is made into a bottled liquid medium with a natural pH; transfer the first-grade liquid bacteria to the sterilized bottled liquid medium according to the aseptic operation process, put it in a room temperature of 25°C, and cultivate it in the dark for 30 days, and then it can be cultiva...

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Abstract

The invention discloses a strain of armillaria mellea YN01 (WT). The preservation number of the armillaria mellea YN01 (WT) in CCTCC (China Center for Type Culture Collection) is CCTCC NO:M2016611. The armillaria mellea provided by the invention has the advantages that the germination speed is high; the growth speed is high; the culture is easy; the growth power is strong; the co-culture time with bacteria is short, and the like. In the use process, the rhizoma gastrodiae culture period can be shortened; the economic cost is lower; the rhizoma gastrodiae yield can be effectively increased; the economic benefits can be improved; the foundation is laid for the Zhaotong gastrodia elata mutualistic armillaria mellea strain culture and the Zhaotong rhizoma gastrodiae industry development to modernization, professionalization and scale development.

Description

technical field [0001] The invention relates to Armillaria YN01 (WT) and its application, belonging to the technical field of microorganisms. Background technique [0002] Armillaria ( Armillaria mellea ), belonging to Basidiomycotina, Phytomycetes, Agaricales, Tricholomaceae, and Armillaria, widely distributed in forest areas all over the world. It has the effects of improving eyesight, benefiting the lungs, benefiting the stomach, antispasmodic, sedative, dilating blood vessels, lowering blood pressure, etc., and the fruiting bodies, hyphae, and fungal cords can be used as medicine. Armillaria armillaria is a class of higher fungi with important economic value, and it is also a symbiotic fungus of rare Chinese medicinal materials Gastrodia elata and Polyporus. Armillaria Armillaria plays a decisive role in the formation of Gastrodia elata seedlings, the growth of vegetative organs, and the transformation from vegetative growth to reproductive growth. [0003] As we all ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/00C12R1/645
CPCA01G22/00C12N1/145C12R2001/645
Inventor 李昆志包燚操璟璟雷玉珠陈丽梅徐慧妮
Owner KUNMING UNIV OF SCI & TECH
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