Method for establishing halimasch liquid fermentation production HPLC fingerprint pattern and its standard fingerprint pattern

A technology of fermentation products and fingerprints, which is applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2008-07-16
NORTHEAST NORMAL UNIVERSITY
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  • Abstract
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  • Method for establishing halimasch liquid fermentation production HPLC fingerprint pattern and its standard fingerprint pattern
  • Method for establishing halimasch liquid fermentation production HPLC fingerprint pattern and its standard fingerprint pattern
  • Method for establishing halimasch liquid fermentation production HPLC fingerprint pattern and its standard fingerprint pattern

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Embodiment

[0022] 1. Instruments and reagents

[0023] 1.1 Instrument

[0024] American Agilent 1100 high performance liquid chromatograph, DAD detector.

[0025] Chromatographic column: ZORBAX SB-C18 (4.6×150mm, 5μm), Agilent Company, USA.

[0026] 1.2 Reagents

[0027] Methanol was chromatographically pure reagent from Fisher Company of the United States, and trifluoroacetic acid was chromatographically pure reagent from Tedia Company of the United States. The reference substances uridine, adenosine and 5-hydroxymethylfurfural were purchased from China Institute for the Control of Pharmaceutical and Biological Preparations.

[0028] 2. Methods and Results

[0029] 2.1 Preparation of reference substance solution: Weigh 2 mg each of uridine, adenosine and 5-hydroxymethylfurfural into a 2ml volumetric flask, add 50% methanol to dissolve, set the volume to the mark, and use it as a reference solution for later use ;

[0030] 2.2 Preparation of the test product: After the liquid ferme...

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Abstract

The invention provides a building method of an armillaria mellea effective liquid phase fingerprint and a standard fingerprint and relates to the quality management and quality control of an armillaria mellea fermentation product. The standard fingerprint of the armillaria mellea fermentation product is obtained by comparing an HPLC fingerprint of a deep level fermentation product of 10 batches of armillaria mellea liquid, carrying out processing on the obtained data and spectra by utilizing a fingerprint similarity commenting system of a Chinese medicine chromatogram. The method has the advantages of simple and convenient operation, stable and reliable result, high precision and good reproducibility, etc. The built standard fingerprint can effectively represent the quality of the armillaria mellea fermentation product and provides a novel and effective method for the quality control and true-false distinguishing of the armillaria mellea.

Description

Technical field: [0001] The invention relates to a method for quality control of Armillaria liquid fermentation products, and further discloses a method for establishing an HPLC fingerprint of Armillaria liquid fermentation products, as well as standards for Armillaria liquid fermentation products obtained by the method. The HPLC fingerprint spectrum belongs to the technical field of traditional Chinese medicine quality control standards. Background technique: [0002] Naoxinshu Oral Liquid is composed of Armillaria mellea, royal jelly, and honey. Occurs in summer and autumn, can be facultatively parasitic, and is a fungus that can be used for both medicine and food. A large number of studies have found that armillaria has similar pharmacological effects to Gastrodia elata, has sedative and anticonvulsant effects on the central nervous system, can improve blood circulation, increase blood flow in the brain and coronary arteries, and is mainly used for headaches, dizziness, ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34
Inventor 李玉新郑培和李喜文孟祥颖乌垠鲍永利于春雷
Owner NORTHEAST NORMAL UNIVERSITY
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