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Armillaria mellea strain symbiotic with rhizoma gastrodiae and application of stain

A technology of Armillaria and Gastrodia elata, applied in the field of Armillaria and its preparation, to achieve the effect of good affinity

Active Publication Date: 2016-07-27
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there is no Armillaria symbiotica strain of Gastrodia elata in the prior art. The classification of this strain is named Armillarian abs nona T.J.Volk & Burds., and the preservation registration number is: CGMCCNo.11901, and reports on its preparation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of test tube strains:

[0038] 1) The source of Armillaria mellea was selected in the field (collected from different parts of the Gastrodia elata producing area in Zhaotong) as the introduction bacteria, mixed with broad-leaved branches with a diameter of 50-80mm, and planted in forest ponds as fungus materials. In July-August when the seeds are naturally mature, the Gastrodia elata seeds mixed with germination bacteria are evenly spread on the fungus in the pond, and the fresh leaves of broad-leaved trees and sand are covered for cultivation. After 16-24 months, the gastrodia elata in the pond is selected to grow more , The shape of the fungus material culture fungus cable.

[0039] 2) Separation and purification of bacterial cords: select a few segments of bacterial cords that are clustered, suitable in thickness, robust in growth, and white at the top, and placed in a petri dish on the cultivated bacterial material, and inoculated on the slant medium ...

Embodiment 2

[0044] 1. Preparation of test tube strains:

[0045] 1) Select the source of Armillaria mellea in the field: widely collect the source materials of Armillaria mellea from different Gastrodia elata production areas in Zhaotong as the introduction bacteria, mix them with broad-leaved branches with a diameter of 50-80mm and plant them in forest ponds as fungus materials, and use Gastrodia elata in the following year In July-August when the seeds are naturally mature, the Gastrodia elata seeds mixed with germination bacteria are evenly spread on the fungus in the pond, and the fresh leaves of broad-leaved trees and sand are covered for cultivation. After 16-24 months, the Gastrodia elata in the pond is selected for growth. Bacteria source materials with a large number and good shape are used to cultivate bacterial strands.

[0046] 2) Separation and purification of bacterial strands: select several sections of light-colored and robust bacterial strands from the cultivated bacteria...

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Abstract

The invention provides an armillaria mellea strain related to the high yield and stable yield of rhizoma gastrodiae and a preparation method of the strain. The taxonomy name of the strain is A.nabsnona T.J.Volk&Burds., the strain is a newly recorded species of armillaria fungi in China, and the preservation registration number is CGMCC No.11901. The method comprises the steps of strain selection, separation and purification, identification and cultivation. By means of the armillaria mellea strain related to the high yield and the stable yield of rhizoma gastrodiae and the preparation method of the strain, a risk of low yield of rhizoma gastrodiae or 'empty pond' phenomenon of no yield caused by the fact that selected stains are improper is reduced; the strain and gastrodia elata f.glauca have a better dependency, and the original quality of gastrodia elata f.glauca under the large-scale artificial cultivation condition in the region mainly producing gastrodia elata f.glauca can be guaranteed.

Description

technical field [0001] The invention relates to an armillaria symbiotic with Gastrodia elata and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Armillaria fungi (Armillaria), commonly known as hazel mushrooms and corn fungi, belong to the kingdom Fungi, the subphylum Basidiomycotina, the class Hymenomyeetes, the order Agaricales, and the family Physalacriaceae. There are about 40 species of this genus of fungi in the world, and they are widely distributed in China. They are distributed in central China, southwest and northeast regions. Armillaria is an important symbiotic fungus of Gastrodia elata (Gastrodia elata Bl.), a precious traditional Chinese medicine. 95% of the growth period of Gastrodia elata requires Armillaria to provide nutrients in the form of rhizome cords. Studies have shown that Gastrodia elata can be planted with Armillaria strains from different sources, and the yield and quality of Gastrodia elata ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 郭婷杨祝良
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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