Method for producing rhizoma gastrodiae companion armillaria mellea through liquid submerged fermentation

A liquid submerged fermentation and liquid fermentation technology, applied in the field of microorganisms, can solve the problems of low mycelium yield, slow growth of Armillaria strains, and different sizes and quality of strains

Inactive Publication Date: 2014-05-21
彭佳华
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art, by optimizing the culture medium conditions of each link of the liquid submerged fermentation process of Armillaria elata associated with Armillaria elata, to solve the problems of rapid degeneration and slow growth of Armillaria armillaria strains, low mycelium output, and the To solve the problem of different size and quality of species, provide a method for industrial production of high-quality Armillaria cultivars

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Embodiment 1

[0014] 1. Isolation of Armillaria strains: In the main production area of ​​Gastrodia elata in Xiaocaoba, Yiliang, Yunnan, the cords of Armillaria elata around the growth of wild Gastrodia elata were collected for isolation and purification. The strain number is MHL-001, and the strain preservation number is CGMCC No.6758. , classification name: Armillaria sp., preservation time: November 05, 2012, preservation unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures, preservation unit address: Research on Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing Place.

[0015] 2. Cultivation of Armillaria parent species: prepare test tube slant medium, each liter of medium contains the following ingredients: 200g of potato, 18g of glucose, 15g of wheat bran, KH 2 PO 4 2g, MgSO 4 1g, vitamin 10mg, agar 15g, pH 7. The isolated and purified Armillaria strains were inoculated on the slant culture medium of the te...

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Abstract

The invention relates to a method for producing rhizoma gastrodiae companion armillaria mellea through liquid submerged fermentation. The method is characterized in that a production technology of the rhizoma gastrodiae companion armillaria mellea through the liquid submerged fermentation comprises five steps as follows: (1) strain isolation of the armillaria mellea; (2) mother culture of the armillaria mellea; (3) preparation of solid seed bacteria; (4) liquid fermentation culture; and (5) cultivar inoculation and culture of the armillaria mellea. According to the method, various culture media of the armillaria mellea are provided, and problems that the armillaria mellea is rapid in degradation and slow in growth of strains, low in mycelium yield and varied in size and quality of the strains are solved; compared with traditional solid fermentation, the production technology has the advantages that the growth cycle of finished cultivar products of the armillaria mellea is greatly shortened, in addition, the rate of finished products is increased, and large-scale factory production of the high-quality rhizoma gastrodiae companion armillaria mellea is realized.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for producing Gastrodia elata associated Armillaria cultivars by liquid submerged fermentation. Background technique [0002] Gastrodia elata Gastrodia elsta Bl. belongs to Orchidaceae ( Orchidaceae ), without roots and green leaves, can not carry out photosynthesis, is a heterotrophic plant. The whole process of Gastrodia elata's life cycle is provided by two or more types of fungi. The germination of Gastrodia elata seeds depends on seed germination bacteria such as Osmanthus elata for nutrition. Armillaria can produce bacterial strands during the growth process, and the bacterial strands can protrude from the substrate that provides nutrients and enter another substrate that does not support its growth. Yield and quality have a great influence. In order to ensure the quality and yield of Gastrodia elata cultivation, it is necessary to screen out dominant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 不公告发明人
Owner 彭佳华
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