44 results about "Rhizopus stolonifer" patented technology

The invention discloses a preparation method of vinegar residue fermented complex micro organism fungicide. The fungicide is formed by single strain fungicides of bacillusacidocaldarius., rhizopus stolonifer and trichoderma viride by weight ratio of 1:1:1, wherein firstly stock seeds of the single strain fungicides are respectively multiplied by a primary test tube and are respectively subject to secondary liquid culture to obtain bacteria; then 50-60% of rice bran and 50-40% of wheat bran are mixed by weight percent and stirring to be uniform is carried out to obtain mixed material, the cultured bacteria are respectively inoculated according to the volume weight ratio of bacterium and mixed material, thus obtaining single strain fungicide. The complex micro organism fungicide provided by the method can increase fermentation temperature of vinegar residue compost and speed up fermentation, and fermentation finished product thereof has high content of full dose nutrients (nitrogen, phosphorous and potassium) and good nutrition.

The invention relates to a green and efficient biological organic soil improvement agent, in particular to a novel composite biological soil improvement agent. The novel multifunctional composite biological soil improvement agent is characterized by being prepared by uniformly mixing the following raw materials in parts by weight: 20-40 parts of rhizopus stolonifer fermentation liquid, 20-40 parts of trichoderma viride fermentation liquid, 1.5-2.5 parts of water-soluble chitin and 1.5-4.5 parts of potassium fulvic. The soil improvement agent mainly has the functions that the photosynthesis capacity of plants can be enhanced, and the directional transportation of photosynthetic products is facilitated; the root growth is promoted, and the yield is increased by 20% to 30%; the fruits are attractive, the color is bright, and the content of sugar and vitamins is increased; the soil pH value is increased; acidified soil, hardened soil and salinity soil are improved; the soil nutrient is activated, and the soil fertility is released.

The invention relates to a cherry preservative and fresh-keeping microbial preparation and a preparation method and application thereof. The cherry preservative and fresh-keeping microbial preparationis prepared from bacillus velezensis TA-3-BV which is preserved in China General Microbiological Culture Collection Center on July 17, 2020, and the preservation number is CGMCC NO. 20398. The microbial preparation disclosed by the invention can effectively inhibit mycelial growth and spore germination of pathogenic bacteria rhizopus stolonifer and effectively inhibit morbidity of soft rot of sweet cherries at the same time, after the picked cherries are soaked in bacillus velezensis TA-3-BV bacterial suspension, the fruit spoilage process can be delayed, and fruit spoilage is delayed by 2-3days. Meanwhile, through treatment of the bacillus velezensis TA-3-BV, the disease resistance and oxidation resistance of plants can be induced, the basic immunity of cherry fruits is triggered, and anew method is provided for preservation and fresh keeping of the harvested sweet cherries.

The invention mainly relates to microorganism fermenting methods, in particular to a method which prepares a solid FGR microbial inoculum that combines yeast fusion strain (F306), geotrichum candidum link (G361) and rhizopus stolonifer strain (R) by utilizing wastewater and waste residue generated in food industries as well as application thereof. A solid culture medium of the solid microorganism microbial inoculum comprises following dry components by weight percentage: 50 to 80 percent of scum residue, 15 to 30 percent of corncob powder, 8 to 12 percent of bran coat, 0.5 to 0.8 percent of corn meal, 0.1 to 3 percent of ureophil and 0.2 to 4 percent of ammonium sulfate, fresh wastewater of bean curd, bean vermicelli, corn starch or yam starch is added for leading the weight percentage of water to be 45 to 65 percent, limewater with the concentration of 1 percent is used to adjust pH value between 5.5 and 6.5, a mixed strain liquid of the yeast fusion strain, the geotrichum candidum link and the rhizopus stolonifer strain with the weight 8 to 12 percent of the total dry components is added and completely agitated, ventilated agitation and fermentation are implemented in a fermenting pool with dual purposes of fermenting and drying at the temperature of 28 to 32 DEG C for 18 to 24 hours, and hot air is led into the fermenting pool at the temperature of 40 to 60 DEG C for drying, thus producing the solid microbial inoculum that has great application effect in addition agents of beast and bird feedstuff.

The invention discloses a method for mixed fermentation of green brick tea with multi-fungi. The method utilizes rhizopus stolonifer and mucor racemosus Fres together to ferment the green brick tea, and belongs to the field of microbial fermentation technology application. For the rhizopus stolonifer used by the invention, a preservation number is CCTCC M 2017240 and preservation time is May 5, 2017; and for the mucor racemosus Fres, a preservation number is CCTCCM 2017241 and preservation time is May 5, 2017. The mucor racemosus Fres is a fungus belonging to mucor racemosus and is used for the production and fermentation of the green tea brick. The method for the mixed fermentation of the green brick tea with fungi comprises the following steps: culturing two fungi in a liquid state; separately collecting spores of the fungi; putting the spores into the fermented tea pile of green brick tea; and controlling temperature and humidity for fermentation. Compared with traditional fermentation methods of green brick tea, the method for the mixed fermentation of the green brick tea of the invention has the advantages of more mellow, softer and smoother mouth feel of tea products, and shorter fermentation cycle, and greatly improved productivity.

The invention provides a walnut green seedcase extract compound preservative as well as a preparation method and application thereof. The walnut green seedcase extract compound preservative comprises the following components in percentage by weight: 0.01-9% of walnut green seedcase extract, 0.5-3% of chitosan, 0.05-0.12% of emulsifying agent and the balance of water. The preparation method of the compound preservative comprises the following steps: respectively adding the walnut green seedcase extract and chitosan into water according to the weight percentage, and dissolving all the components; sufficiently stirring the components to be uniform; adding the emulsifying agent, and carrying out ultrasonic dispersion to obtain the walnut green seedcase extract compound preservative. The walnut green seedcase extract compound preservative prepared by using the method is used for inhibiting one or a combination of several of penicillium, alternaria, rhizopus stolonifer, botrytis cinerea and fusarium. The walnut green seedcase extract compound preservative provided by the invention is safe, efficient, stable in performance and simple in preparation process.

The embodiment of the invention provides a microbe, an application of the microbe in bio-fertilizer preparation, a bio-fertilizer and an application of the bio-fertilizer. Compared with the existing microbe for the bio-fertilizer and the existing bio-fertilizer in the prior art, the microbe provided by the invention is rhizopus stolonifer PF102, so that the microbe can be used for preparation of the bio-fertilizer; when the bio-fertilizer used for crop cultivation, the bio-fertilizer can improve the output of the crops, greatly reduce the morbidity of the crops and death rate due to disease, and in the meantime improve the growth conditions of the crops such as microbial population at the crop rhizosphere, soil bulk density, soil porosity, length and width of the plant leaf, and fruit quantity.

The invention relates to bacillus velezensis as well as a culture method and application thereof. The endophytic antagonistic strain bacillus velezensis TA-3-BV of sweet cherries is found for the first time, and the nucleotide sequence of 16S rDNA of the bacillus velezensis TA-3-BV is shown as SEQ ID NO.1. The bacillus velezensis TA-3-BV has broad-spectrum antibacterial activity on sweet cherries,has an inhibition effect on various pathogenic fungi and has an excellent inhibition effect on sweet cherry soft rot pathogenic bacteria rhizopus stolonifer, and the inhibition rate can reach 69.82 +/-1.43%. Besides, the bacillus velezensis TA-3-BV has a relatively good inhibition effect on postharvest decay of the sweet cherries, the decay process of the fruits can be delayed, the decay of the fruits is delayed by 2-3 days, and a novel method is provided for postharvest preservation and fresh keeping of the sweet cherries.

The invention discloses pichia membranifaciens Y4 for controlling diseases after peach harvesting and belongs to the field of biological prevention and control of diseases after fruit harvesting. Theyeast is identified as pichia membranifaciens, and the preservation number is CCTCC NO:M 2018110. The use method comprises the following steps: during the use, cultivating the pichia membranifaciens Y4 in an NYDA culture medium at 28 DEG C for 2 to 3 days, activating, performing fermentation cultivation in the NYDB culture medium at 28 DEG C for 20 to 24 hours, repeating the activation process fortwice, centrifuging to obtain thallus, washing for twice and diluting with sterile water to prepare bacterial suspension; perforating the peaches, adding the bacterial suspension and inoculating rhizopus stolonifer spore suspension after 2 hours; and airing at a room temperature, putting into a disinfected plastic basket, sealing with a preservative film, putting into a constant-temperature culture box and storing. Soft rot morbidity and decayed diameter of juicy peaches treated by the yeast are obviously reduced. The pichia membranifaciens Y4 can be applied to prevention and control of the juicy fruit soft rot, can reduce the use of chemical sterilizers and avoids danger to people and environmental pollution caused by the use of the chemical sterilizers.

The invention discloses a fresh-keeping method for tomatoes. The fresh-keeping method includes the following steps: directly immersing freshly harvested tomatoes into a prepared tomato fresh-keeping agent, taking out the tomatoes, naturally air-drying the tomatoes, packing tomatoes into PET plastic bags (about 500 grams/PET plastic bag), sealing the PET plastic bags, and storing the packed tomatoes at 20-25 DEG C. The plant-based fresh-keeping agent for keeping the tomatoes fresh provided by the invention can effectively improve the shelf life of the tomatoes to 14-16 days; the synergistic effect of thymol and salicylic can effectively inhibit spore germination and mycelium growth of Fusarium solani and Rhizopus stolonifer, two main spoilage bacteria of the tomatoes. Therefore, the obtained fresh-keeping agent for the tomatoes is good in fresh-keeping effect, with plant resources as the main raw materials.

The invention discloses a compound biological agent for preventing and treating soil-borne diseases. The compound biological agent contains a mixed fermentation solution which is acquired by deeply fermenting rhizopus stolonifer and trichoderma viride through liquid. Besides, the invention also discloses an application thereof in preventing and treating soil-borne diseases, especially blight and greensickness. The submerged fermentation technique is utilized by the invention, two microorganisms are at high multiplication rate and the exocytosis activity is ultrahigh; the compound biological agent can achieve the technical effect which the pure compound cannot achieve; a large number of thallus and spore can be quickly acquired. The compound biological agent disclosed by the invention has higher bactericidal activity to alternaria alternate fungus, so that the soil-borne diseases, such as blight and greensickness, can be effectively prevented and treated. When the compound biological agent is used together with fertilizer components, the soil stability and the porosity can be obviously promoted.

The invention discloses a rhizopus stolonifer capable of biologically converting ginsenosides. A fermentation product is analyzed by an HPLC (High Performance Liquid Chromatography) system to prepare a component with a reduced color spectrum peak value in an original panaxatriol saponins and a component with an increased peak value in the panaxatriol saponins fermentation product thereof. By isolation and identification, a converted substance in the panaxatriol saponins is confirmed to be ginsenosides Re, a compound (1) in the panaxatriol saponins fermentation product is confirmed to be ginsenosides Rh1, a compound (2) is confirmed to be ginsenosides Rg3, and a compound (3) is confirmed to be ginsenosides Rg2. By speculation, the ginsenosides Re in the panaxatriol saponins is converted into the ginsenosides Rh1 by the black rhizopus or the ginsenosides Re is firstly converted into the ginsenosides Rg2 and then converted into ginsenosides Rh1, and the detailed conversion path is to be further researched.

The invention belongs to the technical field of bioengineering, and particularly discloses a sticky rice and fresh ginger wine brewing method. Sticky rice and fresh ginger wine is prepared from, by weight, 200-300 parts of sticky rice, 20-30 parts of fresh ginger, 0.02-0.03 part of ascorbic acid, 0.1-0.15 part of citric acid, 0.8-1.2 parts of liqueur koji, 0.01-0.03 part of rhizopus stolonifer Cy3 and 160-240 parts of distilled water. Rhizopus stolonifer Cy3 obtained by screening traditional sticky rice koji and traditional liqueur koji are fermented synergically, saccharifying force and alcoholic strength of the sticky rice and fresh ginger wine can be improved, the use rate of the raw materials is increased, production efficiency is improved, and oxidation stain caused by addition of ginger juice can be avoided by adding ascorbic acid and citric acid.

The invention discloses a kit for detecting pathogenic bacteria of mucor lungs as well as a use method and an application of the kit. The kit of the present invention comprises a universal primer capable of being present in the form of a mixture, wherein the universal primer is capable of binding to a common gene conserved region of rhizopus oryzae, rhizomucor micranthum, aureobasidium pullulans, rhizopus stolonifer, and Cunninghamia lanceolata. The kit provided by the invention can realize one-time detection of various mucor fungi, the detection time does not exceed 2 hours, and the specific type of mucor can be further determined in one-time detection.

The invention discloses a strain of rhizopus stolonifer FL-3 and application thereof in extracting pachymaran. The application method comprises the following steps: inoculating rhizopus stolonifer FL-3 spores into poria cocos powder containing sterile water, fermenting at 28-30 DEG C for 2-3 days, and concentrating the fermented product after ultrasonic water extraction to obtain a concentrate; and precipitating the concentrate and performing vacuum drying to obtain the pachymaran. In advance of ultrasonic water extraction of the pachymaran, fermentation pretreatment of the rhizopus stoloniferFL-3 is added. The rhizopus stolonifer FL-3 is separated from a microbial enrichment culture of poria cocos, and an excellent strain obtained by screening moderately grows in the poria cocos powder to generate polysaccharide hydrolase, so that hydrolysis of insoluble polysaccharide structural tissues of the poria cocos promotes dissociation of combined soluble polysaccharide; and compared with aconventional method without fermentation pretreatment, the extraction yield of the pachymaran can be improved by over 30%.

The invention discloses an inhibitor for inhibiting rhizopus stolonifer in tricholoma lobayense cultivation. The inhibitor is obtained through the following preparation method that 1, folium artemisiae argyi and rhizoma acori graminei are mixed in proportion and then put into a distiller, heating burning or steam introducing is conducted on the bottom of the distiller, when the distiller is full of hot water vapor, the water vapor is introduced into a condenser through a condenser pipe, and essential oil and hydrolat are collected through an oil-water separator; 2, high-speed dispersion and emulsion treatment is conducted, wherein the essential oil and the hydrolat which are obtained in the step 1 are put into a high-speed dispersion and emulsion machine to be treated, a high-speed dispersion and emulsion substance is obtained, and then the inhibitor is obtained. By means of the inhibitor for inhibiting the rhizopus stolonifer in tricholoma lobayense cultivation, growth of the rhizopus stolonifer can be well inhibited in the tricholoma lobayense cultivation process, a significant effect is achieved, and meanwhile the tricholoma lobayense yield can be increased.

Use of a fungal biomass for treating industrial wastewaters containing at least one dye, wherein:

• • i. the fungal biomass contains at least the fungal species Rhizopus stolonifer (Ehrenberg) Vuillemin;
  • ii. the fungal biomass absorbs the at least one dye, so as to obtain wastewater that is basically free of the at least one dye.

The invention discloses a ganoderma lucidum substitute material cultivation medium capable of inhibiting rhizopus stolonifer; the ganoderma lucidum substitute material cultivation medium is prepared from the following raw materials in parts by weight: 40-65 parts of wood bits, 30-40 parts of an auxiliary material, 0.5-1.5 parts of gypsum, 0.5-2 parts of sugar, and 0.5-1.5 parts of a folium artemisiae argyi-rhizoma acori graminei mixture, wherein the material/water ratio is 1:(1.3-1.5). The ganoderma lucidum substitute material cultivation medium can well inhibit the growth of the rhizopus stolonifer in the process of cultivation of the ganoderma lucidum, has a significant effect, and also can enhance the yield of the ganoderma lucidum.

The invention discloses a novel application of an acylated phloroglucinol derivative in prevention and treatment of fruit postharvest rot-causing fungi, agricultural plant pathogenic fungi, agricultural bacteria and food-borne pathogenic bacteria, and belongs to the field of bactericides. The twenty acylated phloroglucinol derivatives provided by the invention have the advantages that the twenty acylated phloroglucinol derivatives are used for treating postharvest rot-causing fungi such as botrytis cinerea, penicillium expansum ACCC30898, rhizopus stolonifer CICC 40327, monilinia coerulea ACCC 36263, aspergillus flavus ACCC32601 and the like; various plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, magnaporthe oryzae and phytophthora capsici; agricultural bacteria such as Xanthomonas oryzae pv. Oryzae ACCC 116602, Xanthomonas citri pv. Citri and the like; the compound has obvious bacteriostatic or bactericidal activity on food-borne bacteria such as bacillus cereus BNCC 103930, listeria monocytogenes ATCC 19115, salmonella enteritidis ATCC 14028, escherichia coli O157: H7 ATCC 35150 and the like, and can be developed as a broad-spectrum bactericidal lead compound applied to the agriculture or food industry.

The invention discloses a method for expressing protein in the resting spores of rhizopus stolonifer. The method comprises the following three steps: rhizopus stolonifer culture and spore collection, pretreatment of rhizopus stolonifer spores and electric shock of rhizopus stolonifer spores by using a HDEN method. According to the method, the step of fungal spore germination is avoided, the HDEN electrotransformation technology is adopted, the extracorporeal single-chain coding protein RNA is delivered to penetrate through the cell wall and cell membrane, and the protein is expressed in the resting spores of rhizopus stolonifer. The method disclosed by the invention has simple and quick steps and realizes a perfect effect while the transformation rate exceeds 90%.

The invention belongs to the technical field of biology, and particularly relates to dsRNA for inhibiting rhizopus stolonifer and application of the dsRNA. The sequence of the dsRNA for inhibiting the rhizopus stolonifer is shown as SEQ ID NO.1. The invention discloses application of the dsRNA in preparation of a rhizopus stolonifer inhibitor. Rhizopus stolonifer dcl1 and p450 genes are provided for the first time, non-conserved region sequences of the rhizopus stolonifer dcl1 and p450 genes are selected as target sequences, upstream and downstream primers are designed according to the target sequences and used for amplifying the target sequences, T7 sequences are added to the two ends of the target sequences, and dsRNA is synthesized. A plate bacteriostasis experiment shows that the dsRNA can remarkably inhibit extension and diffusion of rhizopus stolonifer hyphae. It is found that the soft rot symptom of peaches added with the dsRNA is obviously relieved by spraying the dsRNA outside the peach bodies, and therefore the purpose of preventing and treating the soft rot of the peaches is achieved.

The invention discloses an inhibitor capable of inhibiting rhizopus stolonifer in substitute cultivation of ganodorma lucidum. The inhibitor is prepared through the following preparation method: (1) mixing folium artemisiae argyi and rhizoma acori tatarinowii at a ratio; putting the folium artemisiae argyi and the rhizoma acori tatarinowii into a distiller; heating and combusting or introducing steam at the bottom of the distiller; when the distiller is fully filled with the hot steam, enabling the steam to pass through a condensation pipe and introducing the steam into a condenser; then collecting essential oil and hydrolat through an oil-water separator; (2) carrying out high-speed dispersion emulsification treatment: putting the essential oil and the hydrolat, obtained by the step (1), into a high-speed dispersion emulsification machine and treating to obtain a high-speed dispersion emulsified product, namely the inhibitor. By adopting the inhibitor capable of inhibiting the rhizopus stolonifer in the substitute cultivation of the ganodorma lucidum, provided by the invention, the growth of the rhizopus stolonifer can be inhibited very well in a cultivation process of the ganodorma lucidum; the inhibitor has a remarkable effect; meanwhile, the yield of the ganodorma lucidum can be enhanced.

The invention discloses a method for allowing transformed-external-source desoxyribonucleic acid to enter rhizopus stolonifer resting spores. The method includes the steps that rhizopus stolonifer is cultivated, and spores are collected; the rhizopus stolonifer spores are pretreated; the rhizopus stolonifer spores are subjected to electric shock with the HDEN method, and the rhizopus stolonifer spores with led-in to-be-transformed plasmids are obtained. According to the method, the non-germinated spores serve as initiative materials for leading in external source molecules, external source DNA is led into the rhizopus stolonifer resting spores with the HDEN electrotransformation technology, the complex spore germination step can be omitted, the protoplast preparing step or the agrobacterium-mediated transformation step and the like in the traditional method is omitted, the transformation rate is high, and the effect that 6,000 positive transformants or more exist in each transformation reaction system is achieved.