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Method for using Taxus media branches and leaves as raw material to prepare high-purity 10-deacetyl baccatin III

A Mandia yew, deacetylation technology, applied in the direction of organic chemistry, etc., can solve problems such as difficulty for workers to accurately grasp, evaporation loss of organic solvents, incomplete elution, etc., to achieve environmental protection, reduce energy consumption, operation Easy-to-Master Effects

Inactive Publication Date: 2015-11-25
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the process steps of this method can effectively extract and prepare high-purity 10-deacetylbaccatin III, decolorization of activated carbon will adsorb a certain amount of 10-deacetylbaccatin III, resulting in the loss of the product. Washing and eluting with organic solvents will not only cause some products to be lost or not completely eluted during the washing and eluting process, but also cause evaporation loss of organic solvents
In addition, the process is complicated, it is difficult for workers to master it accurately, the process time is long, and the production cost is high

Method used

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  • Method for using Taxus media branches and leaves as raw material to prepare high-purity 10-deacetyl baccatin III
  • Method for using Taxus media branches and leaves as raw material to prepare high-purity 10-deacetyl baccatin III
  • Method for using Taxus media branches and leaves as raw material to prepare high-purity 10-deacetyl baccatin III

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Experimental program
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Effect test

Embodiment 1

[0040] Present embodiment technological step is as follows:

[0041] (1) Rough extraction

[0042] Put the crushed yew branches and leaves of Mandia into an extraction tank, soak them in an ethanol solution with a mass concentration of 40% for 8 hours to obtain a soaking mixture, then soak the soaking mixture for 10 hours and collect the leachate, and extract by percolation , the flow rate of the leachate is controlled at 60L / h, and during the percolation extraction process, an ethanol solution with a mass concentration of 40% should be added to the extraction tank so that the liquid level of the ethanol solution in the extraction tank is higher than that of the crushed leaves of Taxus chinensis. 10cm, when soaked, the ratio of the weight of the crushed Taxus Mandia branch and leaf to the volume of the ethanol solution is 1:4, the weight unit of the crushed Taxus Mandia branch and leaf is kg, and the volume unit of the ethanol solution for L;

[0043] After the percolation e...

Embodiment 2

[0058] Present embodiment technological step is as follows:

[0059] (1) Rough extraction

[0060] Put the crushed branches and leaves of Taxus Mandia into an extraction tank, soak them in a methanol solution with a mass concentration of 50% for 9 hours to obtain a soaking mixture, then soak the soaking mixture for 10 hours and collect the leachate, and extract by percolation , the flow rate of the leaching solution is controlled at 200L / h. During the percolation extraction process, methanol solution with a mass concentration of 50% should be added to the extraction tank so that the liquid level of the methanol solution in the extraction tank is higher than that of the crushed leaves of Taxus chinensis. 8cm, when soaked, the ratio of the weight of the crushed Taxus Mandia branches and leaves to the volume of the methanol solution is 1:3, the weight unit of the crushed Taxus Mandia branches and leaves is kg, and the volume unit of the methanol solution for L;

[0061] After t...

Embodiment 3

[0076] Present embodiment technological step is as follows:

[0077] (1) Rough extraction

[0078] Put the crushed yew branches and leaves of Mandia into an extraction tank, soak them in a methanol solution with a mass concentration of 60% for 10 hours to obtain a soaking mixture, then soak the soaking mixture for 10 hours and collect the leachate, and extract by percolation , the flow rate of the leaching solution is controlled at 400L / h. During the percolation extraction process, methanol solution with a mass concentration of 50% should be added to the extraction tank so that the liquid level of the methanol solution in the extraction tank is higher than that of the crushed leaves of Taxus chinensis. 8cm, when soaked, the ratio of the weight of the crushed Taxus Mandia branches and leaves to the volume of the methanol solution is 1:4, the weight unit of the crushed Taxus Mandia branches and leaves is kg, and the volume unit of the methanol solution for L;

[0079] After the ...

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Abstract

A method for using Taxus media branches and leaves as a raw material to prepare high-purity 10-deacetyl baccatin III comprises the technological steps of (1) coarse extraction, (2) sedimentation, (3) adsorption, (4) dissolution, (5) crystallization and (6) recrystallization. According to the method, a crude product is dissolved by adopting different single solvents through macro-porous resin adsorption to remove a large amount of undissolved impurities, and then the high-purity 10-deacetyl baccatin III is obtained through multiple-time crystallization. The method is simple in process and low in cost and is beneficial to environmental protection, and operation is easy to master.

Description

technical field [0001] The invention relates to a method for preparing high-purity 10-deacetylbaccatin III from branches and leaves of Taxus mandia as raw materials. Background technique [0002] 10-Deacetylbaccatin Ⅲ is a natural compound isolated from Taxus chinensis, which is used as the precursor of anticancer drugs such as paclitaxel, docetaxel and cabazitaxel for artificial semisynthesis. This compound mainly exists in foreign yew varieties such as Europe, and the content of 10-deacetylbaccatin III in its branches and leaves is as high as 0.1-0.18%. The content of baccatin III is lower than that in foreign yew, resulting in the unfavorable situation that my country needs to rely heavily on imported foreign yew branches and leaves for the preparation of high-purity 10-deacetylbaccatin III. [0003] In 1996, my country introduced the Mandia yew (Taxusmedia), which is a natural hybrid species. The male parent is the European yew (Taxusbaccata), and the female parent is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14
CPCC07D305/14
Inventor 王刚蒲擎宇冯永林蒲尚饶沈金亮李伟
Owner SICHUAN AGRI UNIV
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