Method for ultrasonic extraction and separation of 10-deacetyl baccatin III from yew branch leaves

A technology of yew branches and leaves and acetyl baccatine, which is applied in the field of ultrasonic extraction and separation of 10-deacetyl baccatine III, can solve the problems of large solvent consumption and long soaking time, and achieves time saving, high speed and convenience. The effect of industrial production

Active Publication Date: 2008-07-30
江苏拜瑞生物医药科技有限公司
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Problems solved by technology

But this method also has the problems of long soaking time and large solvent consumption.

Method used

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  • Method for ultrasonic extraction and separation of 10-deacetyl baccatin III from yew branch leaves
  • Method for ultrasonic extraction and separation of 10-deacetyl baccatin III from yew branch leaves

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Experimental program
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Embodiment Construction

[0026] Materials used in the test: short branches of European yew (air-dried, water content 5%-10%)

[0027] D101-1 macroporous adsorption resin

[0028] Case 1

[0029] Take 1Kg of dried European yew branches and leaves, crush them, add 10L of 0.1% (w / w) citric acid aqueous solution, put them into an ultrasonic instrument, and perform ultrasonication for 2 hours. Filter out 8L of dark yellow extract, detect by HPLC, contain 1.02g of 10-deacetylbaccatin III. Then add 10L of 0.1% (w / w) citric acid aqueous solution to the leaves, sonicate for 2 hours, and filter out 10L The left and right extracts were detected by HPLC. The second extract contained 0.21 g of 10-deacetylbaccatin III. Added 10 L of 0.1% (w / w) citric acid aqueous solution to the extracted leaves, and ultrasonicated for 2 Hour, obtain about 10L light yellow extract again, detect with HPLC, contain 10-deacetylbaccatin III in the extract for the third time and be 0.08g.

[0030] Case 2

[0031] Get 150Kg of dried ...

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Abstract

The invention discloses an improved method for extracting 10-deacetylbaccatin III (10-DAB) from a yew. A citric acid aqueous solution with the density of 0.1 to 0.5 percent according to the weight percentage is used for extracting crushed yew branches and leaves through continuous ultrasound. The obtained extracting solution is adsorbed through macroporous adsorptive resin, then eluted through ethyl alcohol, and the solvent is distilled to dryness to obtain a fulvous condensed extract. An organic solvent is used for dissolving condensed extract, insolubles is removed, the solvent is distilled to dryness, yellow solid which contains 10-DAB and has the weight ratio of approximately 15-17 percent is then obtained. The purity of recrystal can reach above 98 percent. By utilizing the continuous ultrasound, the extraction time can be greatly reduced, and the solvent can be saved by using the macroporous adsorptive resin, thereby the invention is favorable for large-scale industrial production.

Description

technical field [0001] The invention relates to a method for ultrasonically extracting and separating 10-deacetylbaccatin III from branches and leaves of European yew, in particular using an aqueous solution with a pH value in the range of 3-7.5 for continuous ultrasonic extraction of yew branches and leaves, and then using specific A method for adsorbing and enriching 10-deacetylbaccatin III on a macroporous adsorption resin. technical background [0002] 10-Deacetylbaccatin III (abbreviated as 10-DAB) is the precursor material of semi-synthetic anticancer drugs paclitaxel (Taxol) and docetaxel (docetaxel). In our patent CN1903849, we have disclosed a method for extracting 10-deacetylbaccatin III from European yew, that is, soaking and pulverizing with 5 to 10 times the organic acid dilute solution with a concentration range of 0.5%-3% by weight or Not pre-crushed European yew branches and leaves, the resulting leachate is extracted with an organic solvent. The solvent wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14
Inventor 肖虎韩见生刘永周
Owner 江苏拜瑞生物医药科技有限公司
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