Method for simultaneously extracting paclitaxel and 10-DABIII (10-Deacetyl Baccatin III) from taxus chinensis

A technology of paclitaxel and yew, applied in the direction of organic chemistry, can solve the problems of increasing process difficulty and cost, and achieve the effects of avoiding oxidative degradation, short preparation time and good stability

Inactive Publication Date: 2018-08-31
梅州市中大南药发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of these methods extract paclitaxel and 10-DABIII in the same process flow, thus increasing the difficulty and cost of the process

Method used

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  • Method for simultaneously extracting paclitaxel and 10-DABIII (10-Deacetyl Baccatin III) from taxus chinensis
  • Method for simultaneously extracting paclitaxel and 10-DABIII (10-Deacetyl Baccatin III) from taxus chinensis

Examples

Experimental program
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Effect test

Embodiment 1

[0019] First, crush the branches and leaves of Taxus chinensis to 50 mesh, add 90% volume fraction of ethanol to soak, and the solid-liquid ratio of Taxus chinensis powder and extraction solvent is 1:50 (g / ml). Then, the mixed liquid is poured into the crushing and extracting device for extraction. The extraction temperature is 16°C and the extraction pressure is 200MPa. It is crushed and extracted under low temperature and high pressure, and paclitaxel and 10-DABⅢ in the yew are rapidly dissolved into the extraction solvent. Finally, the supernatant and the precipitate were separated by centrifugation, and the obtained supernatant was the extract of paclitaxel and 10-DABⅢ.

Embodiment 2

[0021] First, crush the branches and leaves of Taxus chinensis to 200 mesh, add 60% volume fraction of ethanol to soak, and the solid-liquid ratio of Taxus chinensis powder and extraction solvent is 1:10 (g / ml). Then, the mixed solution is poured into the crushing extraction device for extraction. After the extraction is completed, the solution is poured into the crushing extraction device for extraction, and the extraction is performed continuously for 3 times. The extraction temperature is 4°C, and the extraction pressure is 50 MPa. Under low temperature and high pressure conditions, Paclitaxel and 10-DABⅢ in Taxus quickly dissolved into the extraction solvent. Finally, the supernatant and the precipitate were separated by centrifugation, and the obtained supernatant was the extract of paclitaxel and 10-DABⅢ.

Embodiment 3

[0023] First, crush the branches and leaves of Taxus chinensis to 100 mesh, add 80% volume fraction of ethanol to soak, and the solid-liquid ratio of Taxus chinensis powder and extraction solvent is 1:20 (g / ml). Then, the mixed solution is injected into the crushing extraction device, and after the extraction is completed, the solution is poured into the crushing extraction device for extraction, continuous extraction is performed twice, the extraction temperature is 8°C, the extraction pressure is 125MPa, and the extraction is crushed and extracted under low temperature and high pressure. Paclitaxel and 10-DABⅢ in Taxus quickly dissolved into the extraction solvent. Finally, the supernatant and the precipitate were separated by centrifugation, and the obtained supernatant was the extract of paclitaxel and 10-DABⅢ.

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Abstract

The invention discloses a method for simultaneously extracting paclitaxel and 10-DABIII (10-Deacetyl Baccatin III) from taxus chinensis. The method comprises the following steps: crushing taxus chinensis leaves into powder; soaking with an extraction solvent; putting a mixed liquid into a crushing extraction device; extracting for 1-3 times; performing crushing extraction at a low temperature andhigh pressure; after paclitaxel and 10-DABIII are rapidly dissolved into the extraction solvent, performing centrifugal separation on supernate and precipitate, thereby obtaining the supernate, that is, extracts of paclitaxel and 10-DABIII. Due to the low temperature and the high pressure, paclitaxel and 10-DABIII can be simultaneously extracted in one process, and in addition, the extraction rateof paclitaxel and 10-DABIII is as high as 96%. The method disclosed by the invention has the advantages of being good in stability, simple to operate, short in preparation time, high in extraction efficiency, safe and non-toxic, and the like, and meanwhile paclitaxel and 10-DABIII can be effectively prevented from oxydative degradation.

Description

technical field [0001] The invention relates to the technical field of extracting chemical components of Chinese medicinal materials, in particular to a method for simultaneously extracting paclitaxel and 10-DABⅢ from yew. Background technique [0002] Taxane compounds are a class of compounds extracted from the bark, roots and leaves of the higher gymnosperm Taxus chinensis, mainly containing paclitaxel, 10-DABⅢ, etc., many of which have been proven to have cytotoxicity and anti-tumor activity, while The natural yew resources used to extract paclitaxel etc. are limited, so the production and supply of natural paclitaxel raw materials are limited, which is far from satisfying the clinical application of paclitaxel. Now people are planting yew trees on a large scale to extract taxanes such as paclitaxel and 10-DABⅢ. How to make full use of yew resources and obtain more effective substances from them is of great significance. Existing methods for extracting effective componen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D305/14
CPCC07D305/14
Inventor 李志良李海池葛发欢张湘东叶岸青
Owner 梅州市中大南药发展有限公司
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