Detection method of jasmonic acid and methyl jasmonate in trace plant fresh sample

A technology for methyl jasmonate and jasmonic acid is applied in the field of detection for the content of jasmonic acid and methyl jasmonate in fresh samples of trace plants, which can solve the problems of long detection time, cumbersome operation, large demand for samples, etc. Reduced time, simplified steps, consistent cost results

Inactive Publication Date: 2011-07-27
HUNAN AGRICULTURAL UNIV
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  • Abstract
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Problems solved by technology

[0004] The present invention overcomes the shortcomings of the original detection technology, such as large demand for samples,

Method used

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  • Detection method of jasmonic acid and methyl jasmonate in trace plant fresh sample
  • Detection method of jasmonic acid and methyl jasmonate in trace plant fresh sample
  • Detection method of jasmonic acid and methyl jasmonate in trace plant fresh sample

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Embodiment Construction

[0012] The present invention will be further described below in conjunction with embodiment.

[0013] 1. Reagents and instruments

[0014] JA, MeJA standard substance (Sigma, chromatographically pure, with a purity of 95%), methanol (Merck, Germany, chromatographically pure), petroleum ether, ethyl acetate, hexane, dichloromethane (domestic analytically pure), reverse carbon ten Eight fillers.

[0015] Agilent 1100 high performance liquid chromatography, G1314A ultraviolet detector, American Thermo TSQ Quantum Ultra tandem mass spectrometry system, equipped with autosampler, column oven and electrospray ion source, Xcalibur 2.0 data processing system.

[0016] 2. Implementation method:

[0017] The detection method of JA (JA) and MeJA (MeJA) in this trace plant fresh sample mainly comprises the following steps to realize:

[0018] 1. Prepare the reversed C18 solid-phase extraction cartridge for the extraction and purification of JA and MeJA;

[0019] 2. Extraction and puri...

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Abstract

The invention discloses a detection method of jasmonic acid (JA) and methyl jasmonate (Me Ja) in a trace plant fresh sample on the basis of solid phase extraction and a tandem mass spectrum, comprising the following steps: (1) preparing a reverse carbon eighteen solid phase extraction small column purified and extracted by JA and MeJA; 2) extracting and purifying JA and MeJA in the plant fresh sample with a solid phase extraction method; and (3) sample detection: after the plant sample is purified and extracted, the content of JA and MeJA is detected by LCMS-MS (Liquid Chromatography Mass Spectrometry-Mass Spectrometry). The invention has the effect that the reverse carbon eighteen solid phase extraction small column is suitable for separating and purifying JA and MeJA, and JA and MeJA are simultaneously purified and separated; and meanwhile, JA and MeJA in a complex sample are separated and detected within the shorter time on the basis of the high sensitivity of the tandem mass spectrum to obtain the same result or result better than the prior detection method. The detection method has the advantages of simple result, low sample demand quantity and the like and is convenient to operate so as to detect JA and MeJA in the trace plant fresh sample at high sensitivity.

Description

technical field [0001] The invention relates to a determination method, which is a detection method aimed at the content of jasmonic acid (JA) and methyl jasmonate (MeJA) in trace plant fresh samples. Background technique [0002] Jasmonic acid compounds including JA, MeJA and other derivatives are plant growth regulating substances widely present in plants. As a class of plant hormones, JA has been recognized by the international academic community. It can induce the expression of plant defense genes, induce the synthesis of various secondary metabolites, inhibit photosynthesis, promote respiration, stomatal closure, leaf aging and shedding, and fruit ripening, etc. Wide range of physiological functions. In recent years, studies have shown that JA is the fastest signaling molecule that plants respond to external stimuli, and it is also a key signaling molecule in the signaling pathway. At present, the research on JA in plants has become a hotspot, and the determination of...

Claims

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Application Information

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IPC IPC(8): G01N30/72
Inventor 肖浪涛蔺万煌童建华黄志刚李合松
Owner HUNAN AGRICULTURAL UNIV
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