A method for creating a low-chalkiness and high-blast-resistant fragrant soft rice germplasm
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A rice blast, high resistance technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of high chalk rate, poor appearance quality, etc.
Active Publication Date: 2021-07-30
JIANGSU LIXIAHE REGION AGRI RES INST
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Nanjing 9108 has poor appearance quality and high chalkiness, while Exiang 2 has excellent appearance quality because it carries the long-grain gene GL7 ( figure 1 )
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Embodiment 1
[0066] Embodiment 1, the selection of parent material
[0067] "Nanjing 9108" is a japonica-type super rice variety recognized by the Ministry of Agriculture in 2015. It was bred by Jiangsu Academy of Agricultural Sciences by crossing Wuxiangjing 14 and Kanto 194 for many years. It is a rare high-yield and delicious variety. . Because of its poor resistance to rice blast, the inventor unit successfully introduced the Pigm gene into Nanjing 9108 through the method of molecular marker-assisted selection through multiple generations of backcrossing and selfing, and bred "Nanjing 9108-Pigm". This strain shows good resistance to rice blast, high yield, high quality, strong aroma and good taste. "Exiang 2" was approved by the Hubei Provincial Crop Variety Approval Committee in 2018. The grain is long and transparent, and its main physical and chemical indicators have reached the national standard of second-class high-quality rice quality standards.
[0070] (1) Take a single rice seedling or a single seed, put it into a 2mL Ep tube, put steel balls in it, freeze it with liquid nitrogen, and grind the leaves or seeds into powder with a vibrating pulverizer;
[0071](2) Immediately pour out the steel balls, and add 600 μL of CTAB extract preheated at 65°C in advance, shake well, incubate at 65°C for 30 minutes, and invert once every 10 minutes;
[0072] (3) Add 600 μL of chloroform-isoamyl alcohol (24:1), mix carefully, and let stand for a while;
[0073] (4) Centrifuge at 12,000rpm for 3-5min, transfer the supernatant to another Ep tube;
[0074] (5) Repeat steps 3 and 4;
[0075] (6) Add 2 / 3 of the supernatant volume pre-cooled isopropanol, let stand at -20°C for 30 minutes, and centrifuge at 12,000 rpm for 5 minutes;
[0076] (7) Discard the supernatant, and wash the precipitate twice with 70% ethanol, 500-700 μ...
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Abstract
The invention provides a method for creating germplasm of low chalkiness and high blast resistance fragrant soft rice, comprising the following steps: using a high blast resistance fragrant soft rice line as the female parent, and using a high-quality long-grain fragrant japonica rice line as the parent Ben, hybridized to get F 1 seed; cultivate F 1 Seeds to obtain self-bred generation population F 2 seed; cultivate F 2 Seed, in F 2 Pigm, Wx-mq and GL7 gene homozygous and / or heterozygous lines were screened out from the lines, and the lines with long grain and good agronomic traits were harvested, and the seeds F of all lines were harvested. 3 ; breed F 3 Seed, in F 3 Among the strains, the pigm, Wx‑mq and GL7 genes are all homozygous, and the strains with long grains and good agronomic traits are screened out. The strains continue to be selfed for multiple generations and combined with rice blast resistance identification, and finally obtain low chalkiness. Baigao fragrant soft rice line resistant to blast. The invention adopts step-by-step molecular screening to realize high-efficiency transfer of genes with excellent appearance quality, thereby creating a new line of fragrant soft rice with low chalkiness and high blast resistance.
Description
technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for creating a low-chalky, high-blast-resistant fragrant soft rice germplasm. Background technique [0002] Rice (Oryza sativa L.) is the most important food crop in my country. In recent decades, my country's rice yield has been greatly improved under the programs of high-yield breeding, super-high-yield breeding, super rice breeding and green super rice breeding. However, with the improvement of national living standards and tastes, there is an increasing demand for green, high-quality and high-resistance varieties. Therefore, cultivating high-quality and high-resistance rice varieties is of great significance for ensuring my country's food security and improving rice quality and rice competitiveness. [0003] Rice quality mainly includes four aspects: milling quality, appearance quality, cooking and eating quality, and nutritional quality. Among t...
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