Preparation method of fragrant soft rice germplasms with low chalkiness and high resistance to rice blast
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A rice blast and aroma type technology is applied in the field of creating low chalky and high blast resistance aroma type soft rice germplasm, and can solve the problems of poor appearance quality and high chalkiness rate
Active Publication Date: 2020-04-17
JIANGSU LIXIAHE REGION AGRI RES INST
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Nanjing 9108 has poor appearance quality and high chalkiness, while Exiang 2 has excellent appearance quality because it carries the long-grain gene GL7 ( figure 1 )
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Embodiment 1
[0066] Embodiment 1, the selection of parent material
[0067] "Nanjing 9108" is a japonica-type super rice variety recognized by the Ministry of Agriculture in 2015. It was bred by Jiangsu Academy of Agricultural Sciences by crossing Wuxiangjing 14 and Kanto 194 for many years. It is a rare high-yield and delicious variety. . Because of its poor resistance to rice blast, the inventor unit successfully introduced the Pigm gene into Nanjing 9108 through the method of molecular marker-assisted selection through multiple generations of backcrossing and selfing, and bred "Nanjing 9108-Pigm". This strain shows good resistance to rice blast, high yield, high quality, strong aroma and good taste. "Exiang 2" was approved by the Hubei Provincial Crop Variety Approval Committee in 2018. The grain is long and transparent, and its main physical and chemical indicators have reached the national standard of second-class high-quality rice quality standards.
[0070] (1) Take a single rice seedling or a single seed, put it into a 2mL Ep tube, put steel balls in it, freeze it with liquid nitrogen, and grind the leaves or seeds into powder with a vibrating pulverizer;
[0071](2) Immediately pour out the steel balls, and add 600 μL of CTAB extract preheated at 65°C in advance, shake well, incubate at 65°C for 30 minutes, and invert once every 10 minutes;
[0072] (3) Add 600 μL of chloroform-isoamyl alcohol (24:1), mix carefully, and let stand for a while;
[0073] (4) Centrifuge at 12,000rpm for 3-5min, transfer the supernatant to another Ep tube;
[0074] (5) Repeat steps 3 and 4;
[0075] (6) Add 2 / 3 of the supernatant volume pre-cooled isopropanol, let stand at -20°C for 30 minutes, and centrifuge at 12,000 rpm for 5 minutes;
[0076] (7) Discard the supernatant, and wash the precipitate twice with 70% ethanol, 500-700 μ...
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Abstract
The invention provides a preparation method of fragrant soft rice germplasms with low chalkiness and high resistance to rice blast. The preparation method comprises the following steps: hybridizing fragrant soft rice strains with high resistance to rice blast as female parents and high-quality long-grain fragrant japonica rice strains as male parents to obtain seeds F1; culturing the seeds F1 to obtain a first-generation selfing population, namely seeds F2; culturing the seeds F2, screening out strains with homozygous and/or heterozygous Pigm, Wx-mq and GL7 genes, long grains and good agronomic traits from the strains F2, and harvesting seeds F3 from all the strains; and culturing the seeds F3, screening out strains with homozygous Pigm, Wx-mq and GL7 genes, long grains and good agronomictraits from the strains F3, and continuously selfing the strains for multiple generations and identifying the rice blast resistance to finally obtain fragrant soft rice strains with low chalkiness andhigh resistance to rice blast. Step-by-step molecular screening is adopted to realize efficient transfer of genes with high appearance quality, so the novel fragrant soft rice strains with low chalkiness and high resistance to rice blast are obtained.
Description
technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for creating a low-chalky, high-blast-resistant fragrant soft rice germplasm. Background technique [0002] Rice (Oryza sativa L.) is the most important food crop in my country. In recent decades, my country's rice yield has been greatly improved under the programs of high-yield breeding, super-high-yield breeding, super rice breeding and green super rice breeding. However, with the improvement of national living standards and tastes, there is an increasing demand for green, high-quality and high-resistance varieties. Therefore, cultivating high-quality and high-resistance rice varieties is of great significance for ensuring my country's food security and improving rice quality and rice competitiveness. [0003] Rice quality mainly includes four aspects: milling quality, appearance quality, cooking and eating quality, and nutritional quality. Among t...
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