Rice blast disease resisting gene Pi1 tightly interlocked molecular marker R112865-1 and application thereof

Abstract

A rice blast disease resisting gene Pi1 tightly interlocked molecular marker R112865-1 and application thereof belongs to the field of molecular biology, relate to molecular markers, particularly to the rice blast disease resisting gene Pi1 tightly interlocked molecular marker R112865-1. The rice blast disease resisting gene Pi1 tightly interlocked molecular marker R112865-1 has a sequence shown as Seq ID No.1. The invention also relates to a primer for amplifying the rice blast disease resisting gene Pi1 tightly interlocked molecular marker R112865-1, application of the rice blast disease resisting gene Pi1 tightly interlocked molecular marker R112865-1 and the primer to rice blast disease resisting gene Pi1 positioning or rice genetic breeding, and a rice breeding method. The rice blastdisease resisting gene Pi1 tightly interlocked molecular marker R112865-1 and the amplification primer can well type rice blast disease resisting gene Pi1 and also can be applied to rice blast diseaseresistance molecular marker-assisted breeding to provide molecular assisted technical support for early determination and screening of rice blast disease resisting characters, thereby be of high significance in theories and practical guidance to acceleration of genetic breeding and improvement of rice varieties.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a molecular marker, in particular to a closely linked molecular marker of rice blast resistance gene Pi1. The invention also relates to a primer for amplifying the molecular marker, and the use of the molecular marker and the primer in positioning rice blast resistance gene Pi1 or rice genetic breeding. Background technique [0002] Rice (Oryza sativa) is one of the most important grain crops in my country. The national rice planting area accounts for about 30% of the grain crop area, and the output is close to half of the total grain output. Rice is the staple food of more than 60% of the population in my country. [0003] Rice blast is one of the important diseases of rice, which can cause a large reduction in yield, and in severe cases, the yield can be reduced by 40% to 50%, and even the grains cannot be harvested. Rice blast is caused by fungal parasitism, and the rice blast f...

AI Technical Summary

Problems solved by technology

[0004] Traditional disease resistance breeding is based on the performance of segregated populations and the experience of breeders. It is usually affected by factors such as environmental conditions, dominant-recessive relationships, and gene epistasis. It is time-consuming, laborious, and inaccurate. From identifying a plant disease-resistant species to It takes more than ten years to breed disease-resistant varieties, and once pathogens attack, they spread much faster than breeding

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