Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting heparin binding protein through immunofluorescence chromatography and preparation method of kit

A heparin-binding protein and immunofluorescence technology, which is applied to a kit for detecting heparin-binding protein by immunofluorescence chromatography and its preparation field, can solve the problems of lag, low sensitivity and low specificity, and achieves easy control and simple operation. Effect

Inactive Publication Date: 2016-05-11
河南生生医疗器械有限公司
View PDF4 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most commonly used traditional indicators for infection detection are: procalcitonin, C-reactive protein, white blood cell count, neutrophil ratio, erythrocyte sedimentation rate, body temperature, etc., but these indicators have varying degrees of lag, low sensitivity, and low specificity Defects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting heparin binding protein through immunofluorescence chromatography and preparation method of kit
  • Kit for detecting heparin binding protein through immunofluorescence chromatography and preparation method of kit
  • Kit for detecting heparin binding protein through immunofluorescence chromatography and preparation method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kit for detecting heparin-binding protein by immunofluorescence chromatography, comprising a first buffer, a second buffer and a reagent card;

[0034] The first buffer is a phosphate buffer containing 1.5ng / μl rabbit anti-human heparin-binding protein antibody; the second buffer is a chicken anti-rabbit secondary antibody labeled with 0.12 μg / μl fluorescein isothiocyanate Phosphate buffer; Wherein the pH=7.2 of the phosphate buffer, the phosphate concentration is 50mmol / L;

[0035] Described reagent card comprises test strip, and described test strip is as figure 1 As shown, the width is 5mm, including the plastic backing plate 1, the nitrocellulose membrane 2 is set on the plastic backing plate, the detection line 3 and the quality control line 4 are set on the nitrocellulose membrane 2 at an interval of 7 mm left and right, and the nitrocellulose on the left side of the detection line 3 A glass cellulose membrane 5 is arranged on the plain membrane 2, an absorbent...

Embodiment 2

[0044] A kit for detecting heparin-binding protein by immunofluorescence chromatography, comprising a first buffer, a second buffer and a reagent card;

[0045] The first buffer is a phosphate buffer containing 0.83ng / μl rabbit anti-human heparin-binding protein antibody; the second buffer is a chicken anti-rabbit secondary antibody labeled with 0.05 μg / μl fluorescein isothiocyanate Phosphate buffer; Wherein the pH=6.5 of the phosphate buffer, the phosphate concentration is 80mmol / L;

[0046] Described reagent card comprises test paper strip, and described test paper strip width is 3mm, comprises plastic backing plate, and nitrocellulose film is arranged on plastic backing plate, and testing line and quality control line are set on the left and right intervals of 5mm on nitrocellulose film, detect A glass cellulose membrane is set on the nitrocellulose membrane on the left side of the line, absorbent paper is set on the nitrocellulose membrane on the right side of the quality ...

Embodiment 3

[0055] A kit for detecting heparin-binding protein by immunofluorescence chromatography, comprising a first buffer, a second buffer and a reagent card;

[0056] The first buffer is a phosphate buffer containing 2ng / μl rabbit anti-human heparin binding protein antibody; the second buffer is a chicken anti-rabbit secondary antibody containing 0.2 μg / μl fluorescein isothiocyanate labeled Phosphate buffer; wherein the pH of the phosphate buffer is 7.3, and the phosphate concentration is 30mmol / L;

[0057] Described reagent card comprises test paper strip, and described test paper strip width is 6mm, comprises plastic backing plate, and nitrocellulose film is set on plastic backing plate, and detection line and quality control line are set on the left and right intervals of 10mm on the nitrocellulose film, detect A glass cellulose membrane is set on the nitrocellulose membrane on the left side of the line, absorbent paper is set on the nitrocellulose membrane on the right side of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Widthaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of medical supplies, and particularly discloses a kit for detecting heparin binding protein through immunofluorescence chromatography and a preparation method of the kit. The kit comprises a first buffer solution, a second buffer solution and a reagent card. The first buffer solution is a phosphate buffer solution containing rabbit anti-human heparin binding protein antibodies, wherein the concentration of the rabbit anti-human heparin binding protein antibodies is 0.83-2 nanograms per microliter. The second buffer solution is a phosphate buffer solution containing fluorescently-labeled affinipure chicken anti-rabbit, wherein the concentration of fluorescently-labeled affinipure chicken anti-rabbit is 0.05-0.2 microgram per microliter. The reagent card comprises a plastic cushion plate. A nitrocellulose membrane is arranged on the plastic cushion plate. A detection line and a quality control line are arranged on the nitrocellulose membrane in a spaced and left-right mode, a glass cellulose membrane is arranged on the portion, on the left side of the detection line, of the nitrocellulose membrane. A piece of water absorption paper is arranged on the portion, on the right side of the quality control line, of the nitrocellulose membrane. A blood filter membrane is arranged on the glass cellulose membrane. The detection line is wrapped by rabbit anti-human heparin binding protein antibodies. The quality control line is wrapped by goat anti-rabbit antibodies. The kit has a good linear range and is high in flexibility and accuracy.

Description

technical field [0001] The invention relates to the technical field of medical supplies, in particular to the technical field of medical supplies for biological and medical testing, in particular to a kit for detecting heparin-binding protein by immunofluorescence chromatography and a preparation method thereof. Background technique [0002] Infectious diseases are a class of diseases that pose a serious threat to health. Sepsis and some serious infectious diseases have become the main causes of morbidity and death. The most commonly used traditional indicators for infection detection are: procalcitonin, C-reactive protein, white blood cell count, neutrophil ratio, erythrocyte sedimentation rate, body temperature, etc., but these indicators have varying degrees of lag, low sensitivity, and low specificity Defects. In the prevention and treatment of infectious diseases, if early and accurate diagnosis can be made, reasonable countermeasures can be taken early, thereby improv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/68G01N33/531G01N33/6854
Inventor 王嘎程自卿王辉武
Owner 河南生生医疗器械有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com