Immune colloidal gold test strip for detecting organic phosphorus pesticide and preparation method thereof

Abstract

The invention discloses an immune colloidal gold test strip for detecting organic phosphorus pesticide and a preparation method of the test strip, and belongs to the technical field of pesticide detection. The test strip comprises a PVC (Polyvinyl Chloride) backing, a sample pad, a colloidal gold combining pad, a detection line, a quality control line, a nitrocellulose membrane and a water absorbing pad, wherein the colloidal gold combining pad is wrapped by a universal organic phosphorous pesticide antibody marked by colloidal gold; conjugate of universal organic phosphorous pesticide hapten and carrier protein is sprayed on the detection line; an anti-mouse or anti-rabbit IgG antibody is sprayed on the quality control line. The test strip has the characteristics of high specificity, high sensitivity, good repeatability and the like, and can be popularized and applied to organic phosphorus pesticide residue detection on vegetables, fruits and the like.

Description

technical field [0001] The invention relates to the technical field of pesticide detection, in particular to a test strip for detecting organophosphorus pesticide residues using antibody immune colloidal gold technology and a preparation method thereof. Background technique [0002] At present, the main methods for the detection of pesticide residues in my country still rely on liquid chromatography (HPLC), gas chromatography (GC), liquid chromatography-tandem mass spectrometry (HPLC / MS / MS), gas chromatography-mass spectrometry (GC / MS). ) and other large-scale modern analytical instruments. Although these instruments have high detection sensitivity, they are expensive and require highly trained professional technicians to operate and use. At the same time, it takes several hours or even overnight from sample processing to final results. The operation process during this period requires several steps to more than ten steps, which cannot meet the timely and rapid detection of la...

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Problems solved by technology

[0002] At present, the main methods for the detection of pesticide residues in my country still rely on liquid chromatography (HPLC), gas chromatography (GC), liquid chromatography-tandem mass spectrometry (HPLC / MS / MS), gas chromatography-mass spectrometry (GC / MS). ) and other large-scale modern analytical instruments. Although these instruments have high detection sensitivity, they are expensive and require highly trained professional technicians to operate and use. At the same time, it takes several hours or even overnight from sample processing to final results. The operation process during this period requires several steps to more than ten steps, which cannot meet the timely and rapid detection of large-scale samples or temporary samples technically and economically, so that people's requirements for food quality and safety in daily life cannot be guaranteed. Therefore, research on vegetable And the rapid detection technology of pesticide residues in food has become a concern of the competent departments at all levels and the general residents. Therefore, sensitive, rapid and accurate detection equipment and methods for organophosphorus should be developed as soon as possible for the detection of vegetables, fruits, tissues and other samples. Organophosphorus residues are of great significance

In the existing similar technology, the manufacturer of the ELISA kit is mainly EnviroLogix Inc. of the United States. The test sample is water body, which cannot be used to detect the organophosphorus pesticide residues in fruits and vegetables, and the minimum detection limit is 0.023ppb; in addition, the domestic , and many other manufacturers have developed and produced a variety of quick test cards, which generally use the principle of cholinesterase inhibition to detect organophosphorus pesticide residues in fruits and vegetables; although such quick test cards apply the national standard GB / T5009.199, for rapid detection of organophosphorus and carbamate pesticide residues in vegetables, but still not very accurate and convenient

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