Immune nano gold test strip for quickly detecting four nitrofuran medicaments

A technology of nitrofuran and nano-gold, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problems of inability to detect at the same time

Inactive Publication Date: 2011-09-14
河北省兽药监察所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current existing methods and technologies cannot detect these four nitrofuran drugs at the same time, because their immunogens only target one drug when they are synthesized, which has great shortcomings

Method used

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  • Immune nano gold test strip for quickly detecting four nitrofuran medicaments
  • Immune nano gold test strip for quickly detecting four nitrofuran medicaments
  • Immune nano gold test strip for quickly detecting four nitrofuran medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Synthetic general hapten: such as figure 1 As indicated, weigh 60 mg of 5-nitrofurfural and dissolve it in 3 mL of ethanol (solution A), and weigh 70 mg of hydrazine sulfate and dissolve it in 7 mL of distilled water (solution B). Add liquid A dropwise to liquid B at room temperature, stir and react for 30 minutes to obtain a yellow precipitate. After the above reaction solution was neutralized with saturated sodium carbonate solution to pH 6.0-8.0, the mixture was suction filtered to dryness. The remaining solid was washed with 15mL of distilled water, then suction-filtered to dryness, and dried naturally to obtain the universal hapten. The yield of the synthesis reaction was 74%, the melting point was 243-245°C (the melting point of 5-nitrofurfural was 39°C), and the infrared spectrum characteristic absorption Peaks: 3150, 3129, 3099, 1641, 1349, 1251, 1027, 825, 738 cm -1 , the ultraviolet spectrophotometer scans the full wavelength of 5-nitrofurfural and hapte...

Embodiment 2

[0031] (1) Synthesis of universal hapten: weigh 50 mg of 5-nitrofurfural and dissolve in 5 mL of ethanol (solution A); weigh 85 mg of hydrazine sulfate and dissolve in 5 mL of distilled water (solution B); Add it to liquid B, stir and react for 60 minutes to obtain a yellow precipitate; neutralize the above reaction liquid with saturated sodium carbonate solution to pH 6.0-8.0, then filter the mixture to dryness; wash the remaining solid with 20 mL distilled water, Suction filtration to dryness, and natural drying to obtain the universal hapten;

[0032] (2) Synthetic immunogen: Weigh 15 mg of the hapten and dissolve it in 5 mL of methanol, and add dropwise to 5 mL of phosphate buffer containing 30 mg of bovine serum albumin (0.01 mol / L, pH 7.0-7.4 ); then add 60 μL of 25% glutaraldehyde solution dropwise, react at room temperature for 4 hours; then dialyze in 4°C normal saline for 72 hours, and store the dialysate in a freezer;

[0033] (3) Synthesis of capture antigen: weig...

Embodiment 3

[0040] (1) Synthesis of universal hapten: weigh 80 mg of 5-nitrofurfural and dissolve in 4 mL of ethanol (solution A); weigh 100 mg of hydrazine sulfate and dissolve it in 10 mL of distilled water (solution B); Add it to liquid B, stir and react for 40 minutes to obtain a yellow precipitate; neutralize the above reaction liquid with saturated sodium carbonate solution to pH 6.0-8.0, then filter the mixture to dryness; wash the remaining solid with 10 mL distilled water, Suction filtration to dryness, and natural drying to obtain the universal hapten;

[0041](2) Synthetic immunogen: Weigh 20 mg of the hapten and dissolve it in 10 mL of methanol, and add dropwise to phosphate buffer containing 50 mg of bovine serum albumin (0.01 mol / L, pH7.0-7.4 ); then add 100 μL of 25% glutaraldehyde solution dropwise, and react at room temperature for 4 hours; then dialyze in 4°C normal saline for 72 hours, and store the dialysate in a frozen state;

[0042] (3) Synthesis of capture antigen...

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Abstract

The invention relates to an immune nano gold test strip for quickly detecting four nitrofuran medicaments. The immune nano gold test strip is prepared by the following steps of: synthesizing general hapten, immunogen and captured antigen aiming at the four nitrofuran medicaments comprising nitrofurazone, furaltadone, nitrofurantoin and furazolidone by using 5-nitrofurfural as a raw material, immunizing a domestic rabbit serving as an experimental animal to acquire polyclonal IgG antibody, preparing gold labeled antibody by using nano gold and the polyclonal IgG antibody, coating the gold labeled antibody solution on a Glass33 glass cellulose membrane, namely a combined release pad, coating the captured antigen and goat anti-rabbit antibody on a detection line and a quality control line of an AE99 nitrocellulose membrane respectively, sequentially sticking the AE99 nitrocellulose membrane, the combined release pad, namely the Glass33 glass cellulose membrane, an absorption pad Cotton linters 2668 and a sample pad Glass33 on a polyvinyl chloride (PVC) back board, and finally, cutting the back board into 4*50mm test strips. The test strip can simultaneously and quickly detect the four medicaments comprising nitrofurazone, furaltadone, nitrofurantoin and furazolidone in feed; and the cost of the test strip is less than 5 yuan, so the test strip is suitable to be widely applied in feed detection or food animal detection.

Description

technical field [0001] The invention relates to an immune nano-gold test strip for rapid detection of four nitrofuran drugs, which generally belongs to the field of agriculture (animal husbandry and aquaculture), and specifically belongs to the field of animal-derived product safety detection / veterinary drug residue detection. Background technique [0002] Furazolidone, nitrofurazone, nitrofurantoin, and furaltadone are chemically synthesized broad-spectrum nitrofuran antibacterial drugs, but because their original drug and metabolites have carcinogenic and mutagenic effects, they have been listed as banned by the Ministry of Agriculture of China. Drugs for food animals. However, these drugs are often added to animal feed for illegal use, and the resulting veterinary drug residues will seriously affect the safety of animal products and threaten the health of consumers. Therefore, detection of such drugs illegally added in feed can protect the quality and safety of animal pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/532G01N33/558
Inventor 王建平赵彦岭刘聚祥王萍张治洲刘怡菲
Owner 河北省兽药监察所
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