Test strip for detecting HIV antibodies in spittle and preparation method thereof
A technology of test strips and antibodies, applied in the field of medical testing, can solve the problems of low HIV antibody concentration in saliva and inconspicuous test results, and achieve the effects of avoiding false negatives or missed tests, increasing detection sensitivity, and increasing the proportion of content
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Embodiment 1
[0045] Embodiment 1 The present invention detects the test strip of HIV antibody in saliva
[0046] Such as figure 1 Shown is a test strip for detecting HIV antibodies in saliva according to the present invention. Comprising a sample pad 1, a glass fiber membrane 2 tightly connected to one end of the sample pad 1 containing colloidal gold particle markers, a nitrocellulose membrane 3 closely connected to the other end of the glass fiber membrane 2, and the fiber The other end of the plain film 3 is closely connected to the absorbent paper 4, the sample pad 1, the glass fiber membrane 2, the nitrocellulose membrane 3 and the absorbent paper 4 are all pasted on the base plate 7, the sample pad 1 is a glass fiber membrane, The nitrocellulose membrane 3 includes a detection zone 5 coated with HIV recombinant antigens gp41 and gp36 and a control zone 6 coated with goat anti-rabbit IgG antibody, and the colloidal gold particle markers on the glass fiber membrane 2 include micro Si...
Embodiment 2
[0048] Embodiment 2 The preparation method of test strip of the present invention
[0049] Colloidal gold particle labeling is used in the present invention. Among them, the particle diameter of colloidal gold particles is nanometer (30-50nm). The adsorption mechanism of colloidal particles is to use its negatively charged properties under alkaline conditions to combine with positively charged groups of protein molecules by electrostatic attraction. Immunodiagnostic reagents. The preparation steps of the test strip for detecting human immunodeficiency virus (HIV) antibody in saliva are as follows:
[0050] 1. Preparation of anti-human IgG antibody
[0051] Isolate IgG from human cytoplasm by conventional methods, use human IgG to immunize mice, and after fusion of human IgG-immunized mice with myeloma cells, identify them by ELISA method, and culture the identified hybridomas in ascites, screen positive clones, and isolate Purified mouse anti-human IgG antibody. ELISA was ...
Embodiment 3
[0064] Embodiment 3 Utilize the test strip in embodiment 1 to detect the method for HIV antibody in saliva
[0065] In this example, the test strip of Example 1 is used to detect the HIV antibody in the saliva sample. For ease of use, an indicator arrow and a liquid level mark (MAX mark line) are pasted on the glass fiber membrane 2 .
[0066] Include the following steps:
[0067] (1) After collecting the saliva sample, add 1ml of sample treatment solution (0.02MPBS+0.05%Tween-20+1%BSA, pH7.4±0.2) for treatment and then use it for testing. At room temperature, samples should be processed within 1 hour of collection. If the sample cannot be tested in time after processing, the processed sample should be refrigerated at 2-8°C, and the sample should be tested within 12 hours after processing.
[0068] (2) Insert the sample pad 1 into the treated saliva sample solution in the direction of the arrow pointing down the test strip (the liquid level should not exceed the MAX mark li...
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