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Sphingobium yanoikuyae and application thereof in degrading polycyclic aromatic hydrocarbon

A technology of Sphingobacterium and polycyclic aromatic hydrocarbons, which is applied in the field of biological treatment of environmental pollutants, can solve the problems of ineffective degradation of high molecular weight polycyclic aromatic hydrocarbons, achieve considerable economic benefits, reduce the content of polycyclic aromatic hydrocarbons, and achieve good social benefits Effect

Inactive Publication Date: 2011-07-13
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the microorganisms that degrade aromatic hydrocarbons in the past are aimed at low-molecular-weight PAHs, and the degradation effect on high-molecular-weight PAHs is not obvious

Method used

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  • Sphingobium yanoikuyae and application thereof in degrading polycyclic aromatic hydrocarbon
  • Sphingobium yanoikuyae and application thereof in degrading polycyclic aromatic hydrocarbon
  • Sphingobium yanoikuyae and application thereof in degrading polycyclic aromatic hydrocarbon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, Isolation and identification of Sphingobacterium yanoguchi LD29

[0029] 1. Collection of samples

[0030] In October 2008, the crude oil polluted surface layer (<2cm) of sandy soil at the outlet of Meitai No. 1 Well in Fushan Oilfield, Haikou City, Hainan Province was collected on site.

[0031] 2. Isolation and screening of Sphingobacterium yanoguchi LD29

[0032] The PAH-degrading bacteria were acclimated in a water-silicone oil dual-phase system, and the initial concentration of anthracene was 100 mg / L. Aqueous inorganic salt medium (pH 7.5): MgSO 4 ·7H 2 O 0.2g, KH 2 PO 4 1.0g, K 2 HPO 4 1.0g, FeSO 4 0.05g, CaCl 2 0.02g, NH 4 NO 3 1.0g, NaCl 0.5g, deionized water 1000mL.

[0033] After several generations of repeated acclimatization and streak culture in NR solid medium, several strains of pure bacteria were obtained. The pure bacteria were inoculated into liquid medium to verify their degradation ability, and finally a strain capable of degr...

Embodiment 2

[0045] Example 2, Degradation of polycyclic aromatic hydrocarbons by Sphingobacter yanoguchi LD29

[0046] Residual rate = content of polycyclic aromatic hydrocarbons after cultivation ÷ content of polycyclic aromatic hydrocarbons before inoculation × 100%. Degradation efficiency = (content of polycyclic aromatic hydrocarbons before inoculation - content of polycyclic aromatic hydrocarbons after cultivation) ÷ content of polycyclic aromatic hydrocarbons before inoculation × 100%. Degradation efficiency + residual rate = 1.

[0047] The aqueous phase inorganic salt medium is the same as in Example 1.

[0048] A single colony of Sphingobacter yanoguchi LD29 was picked and cultured in an aqueous inorganic salt medium with pH=7.5 at 30° C. for 2 days to obtain a bacterium liquid of Sphingobacter yanoguchi LD29.

[0049] 1. Degradation of a single compound by Sphingobacter yanoguchi LD29

[0050] Inoculate 1mL of Sphingobacterium yanoguchi LD29 bacteria solution into 10mL of aqu...

Embodiment 3

[0055] Embodiment 3, the effect of adding nutrients on the degradation rate of Sphingobacterium yanoguchi LD29 fluoranthene

[0056] The aqueous inorganic salt culture medium is the same as in Example 1, and the nitrogen concentration is 350 mg / L.

[0057] Degradation rate = (C 0 -C 1 ) / C 0 ×100%. Residual rate = C 1 / C 0 ×100%. Degradation rate + residual rate = 1.

[0058] A single colony of Sphingobacter yanoguchi LD29 was picked and cultured in an aqueous inorganic salt medium with pH=7.5 at 30° C. for 2 days to obtain a bacterium liquid of Sphingobacter yanoguchi LD29.

[0059] 1. The effect of adding glucose on the degradation rate of fluoranthene of Sphingobacter yanoguchi LD29

[0060] 1. Prepare 1000mg / L glucose mother solution (glucose content is 1000mg / L) with deionized water, and sterilize at 115°C for 20min.

[0061] 2. Prepare 5 test tubes, add 0.5mL, 1.0mL, 2.0mL, 5.0mL sterilized glucose mother solution to 4 test tubes respectively, then add fluoranthe...

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Abstract

The invention discloses a Sphingobium yanoikuyae and an application thereof in degrading polycyclic aromatic hydrocarbon. The Sphingobium yanoikuyae provided by the invention is named as LD29 with the strain collection number of CGMCC No.4400. The Sphingobium yanoikuyae LD 29 can grow and breed by respectively taking phenanthrene, anthracene, fluoranthene and pyrene as the only carbon source and energy source under the aerobic condition. In addition, the Sphingobium yanoikuyae LD 29 has a degrading effect on mixed polycyclic aromatic hydrocarbon, especially on pentacyclic benzopyrene [alpha] in the mixed polycyclic aromatic hydrocarbon. The Sphingobium yanoikuyae can be used for repairing soil and the water body polluted by the polycyclic aromatic hydrocarbon, and is quick, convenient and environmentally-friendly, and can be used for reducing the content of the polycyclic aromatic hydrocarbon in the environment, thereby providing new microorganism resources for bioremediation for polycyclic aromatic hydrocarbon pollution, and having an important value for environmental governance as well as considerable economic benefit and good social benefit.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and relates to a strain of Sphingobacterium yanoguchi and its application in degrading polycyclic aromatic hydrocarbons. Background technique [0002] Polycyclic Aromatic Hydrocarbons (PAHs for short) are aromatic hydrocarbons containing two or more benzene rings. At room temperature, pure polycyclic aromatic hydrocarbons are solid, and their overall characteristics are high melting point, high boiling point, and low water solubility. The water solubility of polycyclic aromatic hydrocarbons decreases with the increase in the number of benzene rings, and their bioavailability and degradability It also decreases and stays in the environment for a long time. [0003] PAHs come from nature and human activities. Polycyclic aromatic hydrocarbons are a type of organic matter that exists in nature. They can be synthesized by some algae, plants, and degrading bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34
Inventor 李季刘芳梁金松付博
Owner CHINA AGRI UNIV
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