Method for detecting lactulose in milk

A detection method, lactulose technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult separation of lactose and lactulose, difficult quantitative analysis, and strict eluent concentration requirements, achieving separation and good response value

Active Publication Date: 2016-07-20
SHANGHAI BINO TESTING TECH SERVICE CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The traditional analytical methods for lactulose include chromatography and enzyme analysis. However, due to the large amount of lactose in milk, it will affect lactulose, making it difficult for these two methods to perform accurate quantitative analysis.
With the continuous maturity of ion chromatography technology in recent years, the use of ion chromatography to determine sugars has become a new way to determine lactulose, but the separation of lactose and lactulose is still a difficult problem. quite demanding

Method used

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  • Method for detecting lactulose in milk
  • Method for detecting lactulose in milk
  • Method for detecting lactulose in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Add lactulose standard solution to the weighed milk blank sample and shake well to prepare the lactulose spiked sample of this embodiment.

[0041] (1) Sample pretreatment

[0042] Pipette 10.00mL of the milk sample to be tested into a 150mL Erlenmeyer flask, add 2mL of potassium ferrocyanide solution with a concentration of 200g / L, 2mL of zinc sulfate solution with a concentration of 200g / L and 6.5mL of buffer [0.34mol / L Disodium hydrogen phosphate (Na 2 HPO 4 )+0.07mol / L sodium dihydrogen phosphate (NaH 2 PO 4 ), pH=7.5], each addition of reagents should be fully shaken, after all additions, let stand for 30 minutes, filter, discard the initial filtrate, and collect the filtrate.

[0043] Take 5 mL of the filtrate into a 10 mL volumetric flask, add 100 μL of β-D-galactosidase suspension, shake well, cover, and incubate at 50°C for 1 hour. Add 2.0mL of buffer solution [0.75mol / L triethanolamine hydrochloride (C 6 h 15 NO 3 ·HCl)+0.01mol / L magnesium sulfate (MgS...

Embodiment 2

[0050] Add lactulose standard solution to the weighed blank sample of school drinking milk and shake well to prepare the lactulose spiked sample of this embodiment.

[0051] (1) Sample pretreatment

[0052] Pipette 50.00 mL of the student milk sample to be tested in a 250 mL Erlenmeyer flask, add 5 mL of potassium ferrocyanide solution with a concentration of 200 g / L, 5 mL of zinc sulfate solution with a concentration of 200 g / L, and 10 mL of buffer [0.34 mol / L Disodium hydrogen phosphate (Na 2 HPO 4 )+0.07mol / L sodium dihydrogen phosphate (NaH 2 PO 4 ), pH=7.5], each addition of reagents should be fully shaken, after all additions, let stand for 30 minutes, filter, discard the initial filtrate, and collect the filtrate.

[0053] Take 5 mL of the filtrate into a 10 mL volumetric flask, add 100 μL of β-D-galactosidase suspension, shake well, cover, and incubate at 50°C for 1 hour. Add 2.0mL of buffer solution [0.75mol / L triethanolamine hydrochloride (C 6 h 15 NO 3 ·HCl)...

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Abstract

The invention relates to a method for detecting lactulose in milk.The method comprises the steps that lactose and lactulose in the milk react and are hydrolyzed into galactose, glucose and fructose through beta-D-galactosidase, glucose oxidase is added to oxidize most of the glucose into gluconic acid, finally catalase is added to remove hydrogen peroxide, and a testing solution is obtained; an ion chromatograph with an ampere detector is used for analyzing the testing solution, and the content of the lactulose is obtained.According to the method, the content of the lactulose in the milk can be accurately and sensitively measured by means of easy and convenient operation.

Description

technical field [0001] The invention belongs to the field of lactulose detection, in particular to a method for detecting lactulose in milk. Background technique [0002] Lactulose is the isomerization product of lactose in the process of heat treatment of milk. It is an important indicator for evaluating the degree of sterilization and storage time of milk. Therefore, the accurate determination of lactulose in milk is even more important. [0003] The traditional analytical methods for lactulose include chromatography and enzyme analysis. However, due to the large amount of lactose in milk, which will affect lactulose, it is difficult for these two methods to perform accurate quantitative analysis. With the continuous maturity of ion chromatography technology in recent years, the use of ion chromatography to determine sugars has become a new way to determine lactulose, but the separation of lactose and lactulose is still a difficult problem. The requirements are quite dem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨晓敏王建军孟瑾郑小平何亚斌高勇兴
Owner SHANGHAI BINO TESTING TECH SERVICE CO LTD
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