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Separation method of silkworm pupa oil polyunsaturated fatty acid by gradient freezing crystallization

A technology of unsaturated fatty acid and gradient freezing, applied in the field of edible oil, to achieve the effects of high product yield, simple operation and avoidance of oxidation

Inactive Publication Date: 2010-11-24
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the shortcomings of the above method for separating polyunsaturated fatty acids from silkworm chrysalis oil, and to provide silkworm chrysalis oil with simple operation, low operating temperature, difficult oxidation of unsaturated fatty acids, high purity and high yield of polyunsaturated fatty acids Gradient Freeze Crystallization Separation Method of Polyunsaturated Fatty Acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Taking silkworm chrysalis 300g as an example, other raw materials and gradient freezing crystallization thereof used as an example, the method for separating polyunsaturated fatty acids of silkworm chrysalis oil is as follows:

[0027] 1. Silkworm chrysalis pretreatment

[0028] Take 300g of silkworm chrysalis and put them into a blast drying oven, dry them at 80-120°C until their water content is 6%-10%, grind them into powder with a pulverizer, and pass through a 30-80 mesh sieve to obtain silkworm chrysalis powder.

[0029] 2. Ultrasonic extraction of silkworm chrysalis oil

[0030]Put 300g of silkworm chrysalis powder into a container, add 1200g of n-hexane, the mass ratio of silkworm chrysalis powder to n-hexane is 1:4, heat up to 40-50°C, use ultrasonic generator power of 140W for 35 minutes, and use 3- 5 layers of 100-250 mesh gauze are filtered, the filtrate is centrifuged with a centrifuge at 1500-4000 rpm for 10-30 minutes, and the supernatant is distilled un...

Embodiment 2

[0042] Taking silkworm chrysalis 300g as an example, other raw materials and gradient freezing crystallization thereof used as an example, the method for separating polyunsaturated fatty acids of silkworm chrysalis oil is as follows:

[0043] In step 2 of the ultrasonic extraction of silkworm chrysalis oil in this embodiment, put 300 g of silkworm chrysalis powder into a container, add 900 g of n-hexane, the mass ratio of silkworm chrysalis powder to n-hexane is 1:3, heat up to 40-50 °C, and Ultrasonic treatment with a power of 150W was performed for 30 minutes, and other steps in this step were the same as in Example 1. In the urea inclusion treatment step 5, 50 g of mixed fatty acids of silkworm chrysalis oil and 250 g of urea ethanol solution with a mass fraction of 25% are packed into a container and mixed evenly. 1:5, inclusion at 0°C for 12 hours, other steps of this step are the same as in Example 1. In step 6 of gradient freezing and crystallization, 10 g of unsaturat...

Embodiment 3

[0045] Taking silkworm chrysalis 300g as an example, other raw materials and gradient freezing crystallization thereof used as an example, the method for separating polyunsaturated fatty acids of silkworm chrysalis oil is as follows:

[0046] In step 2 of ultrasonic extraction of silkworm chrysalis oil in this embodiment, put 300 g of silkworm chrysalis powder into a container, add 1500 g of n-hexane, the mass ratio of silkworm chrysalis powder to n-hexane is 1:5, heat up to 40-50 °C, and Ultrasonic treatment with a power of 120W was performed for 40 minutes, and other steps in this step were the same as in Example 1. In the urea inclusion treatment step 5, 50 g of mixed fatty acids of silkworm chrysalis oil and 750 g of urea ethanol solution with a mass fraction of 25% are packed into a container and mixed evenly, the mass ratio of mixed fatty acids of silkworm chrysalis oil to 25% urea ethanol solution with a mass fraction 1:15, inclusion at -10°C for 6 hours, other steps of...

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Abstract

The invention discloses a separation method of silkworm pupa oil polyunsaturated fatty acid by gradient freezing crystallization, comprising the following steps of preprocessing silkworm pupas, extracting silkworm pupa oil with ultrasonic waves, preparing silkworm pupa oil mixed fatty acid, preparing a urea ethanol solution, and conducting urea inclusion treatment and then gradient freezing crystallization. Compared with the traditional separation methods of the silkworm pupa oil polyunsaturated fatty acid, the problem of fatty acid oxidation caused by overhigh temperature can be avoided because the whole operation process is conducted under low temperature all along. The silkworm pupa oil polyunsaturated fatty acid prepared by the invention contains 96.5% of polyunsaturated fatty acid (including 4.2% of linoleic acid, 91.3% of alpha-linoleic acid, and 1.0% of arachidonic acid), and can be served as principal components of relative drugs or health food. The invention has the advantages of simple processing step, high product yield, excellent quality, high purity of the polyunsaturated fatty acid and the like, and can be used for preparing the polyunsaturated fatty acid from silkworm pupa oil.

Description

technical field [0001] The invention belongs to the technical field of edible oil, and in particular relates to using silkworm chrysalis as a raw material and adopting a gradient freezing and crystallization method to separate polyunsaturated fatty acids from silkworm chrysalis oil. Background technique [0002] Silkworm chrysalis is a resource for both medicine and food. It is the main by-product of the silk reeling industry. Every ton of raw silk produced can produce 1.5 tons of dried silkworm chrysalis. my country is the main producer of silkworms. There are more than 200,000 tons of silkworm chrysalis in China every year. It can be used, but due to the lack of deep processing technology, it is basically used as feed or fertilizer, and the resource utilization rate is low, resulting in great waste. Studies have shown that dried silkworm chrysalis contains about 30% fat, and the content of unsaturated fatty acids in silkworm chrysalis oil is very high, about 75%. It is rich...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/10C11B1/02C11C1/00C11C1/02C11C1/08
Inventor 李建科吴晓霞
Owner SHAANXI NORMAL UNIV
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