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Bacillus megaterium and application thereof

A technology of Bacillus megaterium and plant growth-promoting bacteria, applied to Bacillus megaterium and its application field, can solve the problems of unsatisfactory effect, indole acetic acid-producing ability or phosphorus-resolving ability cannot miraculously promote growth-promoting bacteria, etc. Growth effect, improvement of red soil cultivation environment, and effect of increasing soil available phosphorus content

Inactive Publication Date: 2014-08-20
NANJING BONONG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the Chinese patent No. ZL2012102458617 filed by the applicant in 2012 discloses a strain of Bacillus megaterium JX15 that also has the ability to secrete indole acetic acid and phosphate solubilize, the strain does not have the ability to produce indole acetic acid. Neither the phosphorus-dissolving ability nor the growth-promoting bacterium ZH5 of the present invention can
More importantly, JX15 is selected from fluvo-aquic soil, and its physical and chemical properties, fertility and microbial environment are quite different from those of red soil. Experiments have found that its effect on promoting crop growth in red soil is not satisfactory

Method used

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  • Bacillus megaterium and application thereof
  • Bacillus megaterium and application thereof
  • Bacillus megaterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Medium preparation, including the following four types of medium:

[0042] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-7.2, sterilized at 121°C for 20min.

[0043] LB liquid medium: without agar, other conditions are the same as above.

[0044] Inorganic phosphorus bacteria medium (PKO medium): 5g tricalcium phosphate, 10g glucose, 0.5g ammonium sulfate, 0.3g sodium chloride, 0.3g magnesium sulfate heptahydrate, 0.3g potassium chloride, 0.03g manganese sulfate, heptahydrate Ferrous sulfate 0.03g, pH7.0 agar 20g, distilled water 1000ml, pH7.0~7.2. Sterilize at 121°C for 20min.

[0045] Inorganic salt medium: ammonium sulfate 2.0g; sodium dihydrogen phosphate 0.5g; dipotassium hydrogen phosphate 0.5g; magnesium sulfate heptahydrate 0.2g; calcium chloride dihydrate 0.1g, distilled water 1000mL, pH7.0, sterilized at 121℃ , 20min.

[0046] 2. Isolation of growth-promoting bacteria

[0047] Weigh 10g of the red...

Embodiment 2

[0055] Aerobic test

[0056] Pour the sterilized LB culture medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, use an inoculation needle to pick up the bacteria cultured on the slant, puncture and inoculate into the above culture base (must pierce to the bottom of the tube). Cultivate at 30°C, and observe the results in 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria. The test results show that ZH5 (CGMCCNo.8802) colonies grow along the surface of the agar column, and no colonies grow in the puncture line, which is strictly aerobic.

[0057] Determination of catalase

[0058] Drop 1 drop of 3% H2O2 on a clean glass slide, take 1 ring of 18-24h LB slant culture, smear in H2O2, if there are bubbles, it is positive, otherwise it is negative. The test results showed that ZH5 (CGMCC No.8802) was positi...

Embodiment 3

[0076] In order to further verify the ability and optimal conditions of the plant growth-promoting bacterium ZH5 obtained in Example 1 to produce indoleacetic acid, the following explores the influence of different liquid volumes, pH, different carbon sources, and different nitrogen sources on the production of indoleacetic acid.

[0077]Put 25ml, 50ml, 75ml, 100ml, 150ml of LB liquid medium containing L-tryptophan (100mg / L) into a 250mL Erlenmeyer flask, and inoculate at 1% (v / v) inoculum in logarithmic growth phase After the ZH5, put it at 30°C, 180r·min -1 Cultivate on a shaking table for 24 hours, and measure the amount of IAA produced by a quantitative method. The result is as figure 2 As shown, because the strain ZH5 (CGMCC No.8802) has a good metabolism, the aeration rate affects the efficiency of the strain to produce IAA. When the liquid volume is 50mL, the strain produces the most IAA, and then with the increase of the liquid volume, the yield decreases.

[0078] ...

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Abstract

The invention discloses a Bacillus megaterium and application thereof, and belongs to the field of microorganism. The plant growth-promoting bacteria (Bacillus megaterium ZH5) is preserved on January 22, 2014 in the China General Microbiological Culture Collection Center affiliated to China Committee for Culture Collection of Microorganisms, and has classification name of Bacillus megaterium and preservation number of CGMCC No.8802. The strain is able to maintain high activity in acid environment and high-yield of indole acetic acid and can utilize insoluble phosphate as a phosphorus source for growth to improve the utilization rate of fertilizer, increase the content of available phosphorus in soil and improve the quality of soil especially red soil; high-yield indole acetic acid promotes plant growth and development, and improves the development and fertilizer absorption of the plant roots, and the increase of the available phosphorus content of soil also realizes high utilization rate of phosphorus fertilizer by plant. Therefore, the Bacillus megaterium has good growth promoting effect on plants especially peanut.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a bacillus megaterium and an application thereof. Background technique [0002] Red soil is the soil that develops under the vegetation of tropical and subtropical rainforest, monsoon forest or evergreen broad-leaved forest. Its main feature is that it lacks alkali metals and alkaline earth metals and is rich in iron and aluminum oxides, showing an acid red color. In my country, it is mainly distributed in the low mountain and hilly areas south of the Yangtze River, including: most of Jiangxi and Hunan provinces, southern Yunnan and southeastern Hubei, northern Guangdong and Fujian, and parts of Guizhou, Sichuan, Zhejiang, Anhui, Jiangsu, etc. , and southern Tibet and other places. It accounts for more than 20% of the country's total land area, 40% of the country's population, and 30% of the country's cultivated land. The red soil area is the main grain production area ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/11
Inventor 王华平郑文波李辉信焦加国闫小梅陈雄张舒玄刘满强陈小云胡锋
Owner NANJING BONONG BIOTECH
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