Method for extracting protein selenium from red kidney bean by supercritical CO2

A red kidney bean, supercritical technology, applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., to achieve the effect of increasing the content of protein selenium, obvious extraction effect, and simple process

Inactive Publication Date: 2013-04-17
SHANXI QIANZE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a supercritical CO 2

Method used

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  • Method for extracting protein selenium from red kidney bean by supercritical CO2

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Effect test

Embodiment 1

[0015] Example 1, supercritical CO 2 The method for extracting red kidney bean protein selenium, the steps are as follows: dry the red kidney beans in an oven at 80°C for 1.5 hours, the water content of the dried red kidney beans is 10%, crush the dried red kidney beans through a 40-mesh sieve, Then the sieved red kidney beans are put into a supercritical extraction kettle for extraction. At a temperature of 50°C and a pressure of 1.7Mpa, CO 2 Under the condition of flow rate of 0.12L / min, extracting for 4 hours, red kidney bean protein selenium plant extract was dissolved in supercritical CO 2 In the fluid, enter the separation kettle, and after adjusting the temperature and pressure, the red kidney bean protein selenium plant extract dissolved in the supercritical CO2 fluid is separated, and the CO 2 recycle.

[0016] Determination of protein selenium (organic selenium)

[0017] Instruments and reagents:

[0018] Water: Class 3 water specified in GB / T 6682-2008.

[001...

Embodiment 2

[0048]Embodiment 2, the present invention uses supercritical CO 2 The method for extracting red kidney bean protein selenium plant extract, the steps are as follows:

[0049] a. Dry and pulverize the red kidney beans and put them into an extraction kettle; the drying is carried out in an oven with a temperature lower than 80°C for 2 hours, and the water content of the dried red kidney beans is lower than 10%. Kidney beans are crushed through a 40-mesh sieve,

[0050] b. Supercritical CO 2 Inject into the extraction kettle, at a temperature of 45°C, a pressure of 2.0Mpa, CO 2 The flow rate is: under the condition of 0.10L / min, extract for 4 hours;

[0051] c, CO dissolved with red kidney bean protein selenium plant extract 2 The fluid enters the separation tank, and the red kidney bean protein selenium plant extract is separated by adjusting the temperature 48-60 and the pressure 2.0Mpa-3.0Mpa, CO 2 The gas is compressed and enters the extraction ta...

Embodiment 3

[0053] Embodiment 3, the present invention uses supercritical CO 2 The method for extracting red kidney bean protein selenium plant extract, the steps are as follows:

[0054] a, red kidney beans are dried and pulverized and packed into an extraction kettle;

[0055] b. Supercritical CO 2 Inject into the extraction kettle, at a temperature of 55°C, a pressure of 1.2Mpa, CO 2 The flow rate is: under the condition of 0.15L / min, extract for 2.5 hours;

[0056] c, CO dissolved with red kidney bean protein selenium plant extract 2 The fluid enters the separation tank, and the red kidney bean protein selenium plant extract is separated by adjusting the temperature 48-60 and the pressure 2.0Mpa-3.0Mpa, CO 2 The gas is compressed and enters the extraction tank for recycling.

[0057] After calculation, the organic selenium content of red kidney bean protein selenium was 2.4 μg / mL.

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Abstract

The invention relates to an extraction method of red kidney bean content, in particular relates to a method for extracting protein selenium from red kidney beans by supercritical CO2. The method for extracting the protein selenium from the red kidney beans by the supercritical CO2 solves the technical problem related to effective extraction of the protein selenium from the red kidney beans, and comprises the following steps of: a. drying and crushing the red kidney beans and then adding the red kidney beans into an extraction kettle; b. injecting the supercritical CO2 into the extraction kettle, and extracting for 3.5-4.5 hours under that condition that the temperature is 45-55 DEG C, the pressure is 1.2-2.0MPa and the CO2 flow is 0.10-0.15L/min; and c. separating protein selenium plant extracts from the red kidney beans, compressing the CO2 and then conveying into CO2 into the extraction kettle for cycle use. The content of the protein selenium in the protein selenium plant extract in the red kidney beans, extracted by the method is 2/4mug/mL, and the extraction effect is obvious.

Description

technical field [0001] The invention relates to a method for extracting red kidney bean contents, in particular to a supercritical CO 2 Method for the extraction of selenium from red kidney bean protein. Background technique [0002] Red kidney beans are a special product of Shanxi, with large grains, bright color, and nutritional and medicinal value. Sustained high temperature and relatively concentrated precipitation in summer make it an ideal place for red kidney bean cultivation. The planting area of ​​red kidney beans in Kelan County has reached 130,000 mu, including 20,000 mu of new export demonstration bases, which can earn 3.5 million US dollars of foreign exchange through export. The goal of stabilizing the planting area, improving the quality of kidney beans and increasing farmers' income has been realized. [0003] The red kidney beans produced in Kelan County have been tested to be rich in selenium. Selenium is an important part of some antioxidant enzymes (gl...

Claims

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Application Information

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IPC IPC(8): C07K1/14
CPCY02P20/54
Inventor 段峻廷
Owner SHANXI QIANZE BIOTECH
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