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Method for feeding dichocrocis punctiferalis on large scale

A technology of peach borer and peach borer, which is applied in the field of large-scale breeding of peach borer, can solve the problems of dry and cracked feed, difficulty in obtaining, and less peach borer, and achieve the effects of preventing larvae from escaping, good consistency, and simple ingredients

Active Publication Date: 2018-06-12
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Another aspect of peach borer artificial breeding is artificial feed. At present, some ingredients in the feed formula such as Honda H (Honda H, etc. Appl. Ent. Zool. 1979, 14 (4): 464-468) are not fully disclosed. It is not easy to purchase in our country, and it is difficult to promote and apply
The patent application "Peach Borer Artificial Feed Formula and Preparation Method" (CN103478493A) discloses that it is composed of chestnut powder, corn flour, peach powder, soybean powder, brewer's yeast powder, multidimensional glucose, casein, cholesterol, ascorbic acid, erythromycin, agar Powder, methyl paraben, methyl p-hydroxybenzoate and water. The components of the artificial feed have not been autoclaved during the preparation process, and the feed needs to be replaced every 3 days during the entire feeding process to prevent the feed from becoming moldy. Contamination of larvae, so the raising process is labor-intensive and time-consuming; Another patent application "artificial feed for peach moth moth, preparation method and subculture feeding method" (CN104304694A) discloses a mixture of wheat germ powder, soybean powder, corn seedling dry powder, chestnut dry powder, yeast The artificial feed composed of extract, potassium sorbate, ethyl p-hydroxybenzoate, vitamin C, corn germ oil, trehalose, agar powder and water solves the problems of low population survival rate and poor succession, but Subculture rearing is done in finger tubes, and a maximum of 2 larvae can be placed. These two artificial diets feed a small number of peach moths, which cannot achieve the purpose of mass production of peach moths, and the cost of main materials is high and difficult. Obtained; Xu Lirong (Xu Lirong. Chinese Academy of Agricultural Sciences master's degree thesis 2011) made a slight change on the basis of the corn borer's feed formula and used it to raise the peach moth. 54.88 grains, and the feed appeared to be dry and cracked in the late feeding period
[0005] From the above, it can be seen that the existing artificial breeding methods of the peach borer cannot realize the large-scale breeding of the peach borer, and it is difficult to meet the needs of various experiments on the various stages and larval instars of the peach borer.

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  • Method for feeding dichocrocis punctiferalis on large scale
  • Method for feeding dichocrocis punctiferalis on large scale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The preparation of peach borer artificial feed of the present invention

[0031] Prepare as follows:

[0032](1) Weigh corn flour, corn leaf flour, soybean flour, wheat bran and yeast powder according to the ratio shown in Table 1 and put them into a sterilization bag after stirring evenly. As component A, put component A under high pressure Sterilize at 120°C for 30 minutes in a sterilizer;

[0033] (2) Dissolve the agar in sterile water according to the ratio shown in Table 1, boil it in a pot until it becomes viscous, then add component A, stir evenly, use it as component B, and cool it to 60-70°C for later use;

[0034] (3) Add sucrose, corn oil, vitamin C, and complex vitamin B according to the ratio shown in Table 1 (complex vitamin B was purchased from Tianjin Lisheng Pharmaceutical Co., Ltd., and each tablet contains the main ingredients: vitamin B 1 3 mg, vitamin B 2 1.5 mg, B vitamins 6 0.2 mg, nicotinamide 10 mg, calcium pantothenate 1 mg; ...

Embodiment 2

[0037] Embodiment 2 Contrast test of the method of artificial rearing of peach borer moth of the present invention

[0038] (1) Source of tested insects

[0039] All ages of the tested peach borer came from the laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0040] (2) Experimental design

[0041] (1) Treatment 1: the 1st instar to the 3rd instar larvae were fed with fresh and tender corn ears, and the 3rd instar larvae were fed according to the artificial feed I prepared as shown in Table 1 in Example 1 until pupation.

[0042] (2) Treatment 2: the 1st to 3rd instar larvae were fed with fresh and tender corn ears, and the 3rd instar larvae were fed with artificial feed II prepared as shown in Table 1 in Example 1 until pupation.

[0043] (3) Treatment 3: feed the artificial feed prepared according to the patent application CN103478493A from the 1st instar larvae to pupation.

[0044] (4) Treatment 4: Feed the artificia...

Embodiment 3

[0054] Embodiment 3 large-scale rearing test of peach borer of the present invention

[0055] (1) The preparation of peach moth artificial feed, prepare following peach borer artificial feed 1000mL according to the method described in embodiment 1: its composition and its weight are: corn flour 30g, corn leaf powder 40g, soybean meal 20g, 40g of wheat bran, 20g of yeast powder, 15g of agar, 12g of sucrose, 1g of vitamin C, 0.6g of complex vitamin B, 7.4g of corn oil, 4g of potassium sorbate, and the rest of sterile water.

[0056] (2) large-scale breeding of peach borer:

[0057] (1) The rearing of peach borer adults and the collection of eggs: put a petri dish with a diameter of 10cm at the bottom of the adult cage (the specification is: 45cm × 31cm × 28cm), and put in a petri dish dipped in 10% honey water in the petri dish. Sponge, for the adult peach borer to supplement nutrition. The peach moth borer adults that emerged within 12 hours were paired into adult breeding ca...

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Abstract

The invention discloses a method for feeding dichocrocis punctiferalis on a large scale, and belongs to the field of insect feeding. The method comprises the following four steps: collection and incubation of dichocrocis punctiferalis eggs, feeding of larvas at the age of 1-3 of the dichocrocis punctiferalis, pupation of larvas at the age of 3 to aged larvas, and feeding of adult dichocrocis punctiferalis. Spawning and collection of the dichocrocis punctiferalis are solved by degreasing cotton, spawning capacity of single female dichocrocis punctiferalis is high, and the hatching rate is high;and secondarily, by the method, newly hatched larvas are fed with tender corns, requirements for nutrition of the larvas at the age of 3 are met, meanwhile, escaping of the larvas can be prevented, and a large number of larvas at the age of 3 can be provided for field inoculation tests; and moreover, compositions of an artificial feed in the method are simple, materials are easily obtained, the cost is low, requirements for nutrition of aged larvas can be met, pollution can be avoided easily, feed change frequency is reduced, and the labor and time are saved. By the artificial feed, large-scale artificial subculture feeding of the dichocrocis punctiferalis can be carried out. The method has the advantages of high feeding efficiency, low feeding cost, high survival rate of the dichocrocispunctiferalis and the like.

Description

technical field [0001] The invention belongs to the field of insect feeding methods, and in particular relates to a method for large-scale feeding of the peach borer. Background technique [0002] Peach borer (Conogethes punctiferalis), commonly known as peach spot borer, peach heart borer, peach borer, etc., belongs to the genus Conogethes punctiferalis of Lepidoptera, and mainly damages chestnuts, peaches, plums, pears, apples, pomegranates, and plums. , cherry, citrus, loquat, apricot, persimmon, hawthorn, masson pine, fir, sunflower, soybean, corn, cotton and other fruit trees and crops, with miscellaneous feeding habits and a wide range of hosts, it is an important omnivorous and borer Sexual pests (Wang Zhenying et al. Plant Protection, 2006, (2): 67-69). In recent years, due to changes in climate, farming system and cultivation methods, the population of Peach Borer has shown a rapid upward trend. Peach borer larvae cluster on corn ears, and multiple larvae on the s...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K10/37A23K10/18A23K20/163A23K20/174
CPCA01K67/033A23K10/18A23K10/30A23K10/37A23K20/163A23K20/174A23K50/90Y02P60/87
Inventor 耿亚玲石洁张谦张海剑郭宁刘树森金戈刘粤阳
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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