Determination method and application of volatile active compound having effect of attracting dichocrocis punctiferalis

Abstract

The invention discloses a determination method and application of a volatile active compound having an effect of attracting dichocrocis punctiferalis. The method comprises the following steps: Step 1,determining a trapping source: taking an apple inoculated with penicilliums as the trapping source; Step 2, trapping volatile matters: performing headspace trapping and elution on the volatile matters of the apple inoculated with the penicilliums; Step 3, identifying the volatile matters: identifying the volatile matters trapped by a gas chromatograph-mass spectrometer, so as to determine a compound required to be screened; Step 4, determining an EAG (electroantennogram) electrophysiological response, so as to acquire determination data; Step 5, determining a Y type olfactometer behavior response and calculating a selection percentage; Step 6, analyzing the acquired data, so as to determine that the volatile active compound having the strongest effect of attracting the dichocrocis punctiferalis is isoamyl alcohol. According to the method, the volatile active compound having the better effect of attracting the dichocrocis punctiferalis can be accurately determined, so as to provide anaccurate evidence for producing a dichocrocis punctiferalis attractant having the better attractive effect and provide a powerful guarantee for management of dichocrocis punctiferalis pests.

Description

technical field [0001] The invention relates to the field of preparation of an attractant for the peach borer moth, in particular to a method for determining and application of a volatile active compound capable of attracting the peach borer moth. Background technique [0002] The peach borer, Conogethes punctiferalis (Guenée), belongs to Lepidoptera and Mothidae, and is an omnivorous and boring pest. The reported host plants include more than 40 kinds of fruit trees, forest trees and crops such as chestnut, peach and corn. Since the larvae soon bore into the fruit after hatching and feed on the fruit, it is very difficult to control the larvae. Adult control measures mostly use sex attractants and light traps, and sex attractants are only effective on male moths. If the male moths have completed mating before trapping and killing, the effect on reducing the population base of the next generation will be greatly reduced. Therefore, it has become the focus of scholars at hom...

AI Technical Summary

Problems solved by technology

The reported host plants include more than 40 kinds of fruit trees, forest trees and crops such as chestnuts, peaches, and corns. Since the larvae soon bore into the fruit after hatching and feed on them, it is very difficult to control them at the larval stage.

Sexual attractants and light traps are often used for the control of adult moths, and sexual attractants are only effective for male moths. If the male moths have completed mating before trapping and killing, the effect on reducing the population base of the next generation will be greatly reduced

Images