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35 results about "Dye binding" patented technology

Dye binding, being a dissociation-association equilibrium phenomenon, is influenced by changes in temperature; a rise in temperature increases the dye-albumin dissociation and thus gives lower results. Therefore, standards and sera should be run under identical conditions.

Method for determining microprotein in near infrared wavelength zone based on dye binding principle

A method for detecting microprotein in near-infrared region based on dye binding principle includes adding the aqueous solution of protein in bottle with incremental concentrations, adding acidic buffer solution, diluting with water, adding cyanine solution, standing and scanning absorption spectrum or measuring the increment of light absorption degree. Its advantages are high sensitivity and less interference.
Owner:XIAMEN UNIV

Method for rapidly detecting white spirit based on nanometer material-organic dye colorimetric sensing array

The invention discloses a method for quickly detecting white spirit based on a nano-material organic dye colorimetric sensor array. The method comprises the following steps of introducing six nano-materials and three dyes to construct a nano-material / dye composite colorimetric sensor array, and extracting change values (delta RGB) of array points RGB before and after reaction as detection signals; and processing the data by utilizing pattern recognition and a radial basis function neural network so as to explore the performance of the array detection white spirit. Compared with the prior art, the method has high recognition and detection capacity for different white spirits, combines a nano material with a traditional dye, improves recognition sensitivity of the array, reduces the number of array points, is simple in data processing, has the advantages of simple operation, short test time, high detection efficiency, good sensitivity and the like, and is suitable for popularization and application. The method has a good application prospect in the aspect of on-site real-time detection of white spirit.
Owner:LUZHOU PINCHUANG TECH CO LTD

Near-infrared fluorescent probe compound taking N-pyridine oxide derivative as recognition group as well as preparation and application of near-infrared fluorescent probe compound

The invention discloses a near-infrared fluorescent probe compound taking an N-pyridine oxide derivative as a recognition group as well as preparation and application of the near-infrared fluorescent probe compound. The preparation method specifically comprises the following steps: firstly, oxidizing a pyridine heterocyclic compound into an N-pyridine-2-formaldehyde oxide derivative, and taking the N-pyridine-2-formaldehyde oxide derivative as a recognition group; finally, enabling rhodamine B hydrazide to react with the N-pyridine oxide derivative in an organic solvent, so as to obtain the N-pyridine oxide-rhodamine B near-infrared fluorescent probe. The N-pyridine oxide-rhodamine B type near-infrared fluorescent probe can detect divalent palladium ions with high sensitivity and high selectivity, can realize colorimetric and fluorescent dual-mode detection of the divalent palladium ions in the environment, and can also be used for rapid detection of the divalent palladium ions in cells. Besides, the applied N-pyridine oxide derivative is a new recognition group which is easily available in initial raw material and easy to prepare, and has a wide application prospect in the aspect of detecting other heavy metal ions after being combined with other common dyes.
Owner:GUANGZHOU YUEWANG AGRI CO LTD

Organic dye and preparation method thereof, and sensitized solar cell containing organic dye

The invention relates to an organic dye and a preparation method thereof, and a sensitized solar cell containing the organic dye, and belongs to the technical field of dye-sensitized solar cell. The organic dye has the following structure described in the description, wherein R1 and R2 are independently selected from any one of H, C1-C18 alkyl and C1-C18 alkoxy, R3 and R4 are independently selected from any one of H, C1-C18 alkyl, C1-C18 alkyl phenyl and C1-C18 alkoxy phenyl, R5 and R6 are independently selected from any one of H, F, C1-C18 alkyl, C1-C18 alkyl phenyl, and C1-C18 alkoxy phenyl, and x and y are 0 or 1. The dye has a twisted electronic skeleton and is combined with a side modifying group to make the dye have a better three-dimensional structure at the same time. The sensitized solar cell prepared by the dye has a higher voltage, and the dye can be used as a co-sensitization agent for being combined with other high-efficiency dyes to prepare a device with excellent performance and good light and thermal stability. The preparation method of the organic dye has the advantages of abundant raw material sources, lower cost, good light and thermal stability of the prepared device, and grander industrialized production prospect.
Owner:CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI

Polypeptide probe pair for measuring transpeptidase alpha activity, method for measuring transpeptidase alpha activity and application of both

Relating to the technical field of biological detection, the invention provides a polypeptide probe pair for determination of transpeptidase A activity, a determination method for the transpeptidase Aactivity and application of the two. The polypeptide probe pair for determination of the transpeptidase A activity provided by the invention comprises a first polypeptide probe and a second polypeptide probe that include a reaction zone, a complementary zone and a dye combination zone, has the advantages of strong specificity, good accuracy and high sensitivity, and can achieve the purpose of determining the transpeptidase A activity without modification or labeling treatment. The determination method for the transpeptidase A activity provided by the invention includes: mixing the polypeptideprobe pair for determination of the transpeptidase A activity with a to-be-detected sample in a transpeptidation reaction buffer solution and performing incubation, then using a biarsenical dye to label the incubated reaction product, combining the incubated reaction product with the biarsenical dye to form a compound, and judging the transpeptidase A activity according to the fluorescence levelof the compound. The method is simple and convenient to operate.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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