67 results about "Dye binding" patented technology

Novel fluorescent dye comprising metal oxide nanoparticles are prepared where the nanoparticles are as small as 3 nm or up to 7000 nm in diameter and where the dye is bound within the metal oxide matrix. In some embodiments the invention, novel dyes are covalently attached to the matrix and in other embodiments of the invention a dye is coordinate or ionic bound within the metal oxide matrix. A method for preparing the novel covalently bondable modified fluorescent dyes is presented. A method to prepare silica comprising nanoparticles that are 3 to 8 nm in diameter is presented. In some embodiments, the fluorescent dye comprising metal oxide nanoparticles are further decorated with functionality for use as multimodal in vitro or in vivo imaging agents. In other embodiments of the invention, the fluorescent dye comprising metal oxide nanoparticles provide therapeutic activity and incorporated therapeutic temperature monitoring.

An expression cassette having a fiber specific promoter driving expression of a gene encoding an elastic and plastic protein based polymer having the repetitive amino acid sequence Gly-Val-Gly-Val-Pro (SEQ. ID. NO. 2), a terminator, and selectable marker genes for transforming plant cells and a transgenic cotton plant having fiber cells stably transformed with the gene encoding the protein based polymer wherein the cotton fiber cells exhibit improved water absorption, temperature transition properties, fiber strength, elasticity, and dye binding capacity.

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.

A method for detecting microprotein in near-infrared region based on dye binding principle includes adding the aqueous solution of protein in bottle with incremental concentrations, adding acidic buffer solution, diluting with water, adding cyanine solution, standing and scanning absorption spectrum or measuring the increment of light absorption degree. Its advantages are high sensitivity and less interference.

Hydroxamate substituted azaindoline cyanine dyes, conjugates thereof and methods of using the same are provided. The subject cyanine dyes include an azaindoline ring having a hydroxamate substituent. The dyes may further include a reactive group moieties (RGM) and / or a water soluble group. Also provided are conjugates of the subject dyes. Also provided are tandem conjugates including a fluorescent protein capable of energy transfer to the dye. Methods of detecting an analyte in a sample by contacting the sample with a detection reagent are provided. The detection agent may be a dye-conjugate that specifically binds the analyte, or may be a reactive dye which conjugates to the analyte. Also provided are compositions, e.g., kits, etc., incorporating such dyes which facilitate use in such methods.

Regarding a composite particle in the related art, an antibody and a dye are bound to the surface of the particle. Therefore, it is not possible to bind both the antibody and the dye high in number. Accordingly, the present invention provides a composite particle, wherein a large acoustic wave can be emitted and both the antibody and the dye can be bound high in number. A composite particle including a core particle, a dye bound to the above described core particle, and an antibody, wherein the above described antibody is bound to the above described dye, is suitable for use.

The invention relates to a method for measuring lysine in corn through a near infrared spectrum. The method comprises the following steps of: testing a spectrum, rejecting an abnormal value, establishing an analysis model and the like. By the method, the lysine content can be rapidly and nondestructively measured without pretreatment, and the method is particularly suitable for measuring the lysine content of low-generation materials in the process of breeding high-quality protein corn; compared with lysine content measured results in a dye binding method (DBL), a ninhydrin development process and an amino acid analyzer method, the lysine content measured result in the method verifies the feasibility of a method for measuring the corn through the near infrared spectrum, particularly lysine of the low-generation materials in the process of breeding the high-quality protein corn; moreover, the method is rapid, reliable and practical, and has certain reference value for selecting a method for measuring the lysine content in the process of breeding the high-quality protein corn.

The invention discloses a preparation method of a functional impact-resistant veneer; the veneer is processed through dyeing and drying, resin preparation, resin impregnation and drying balance, so that the veneer has good and lasting impact resistance and attractiveness, and the service life of the veneer is prolonged. Specifically, the problems of cracking, warping deformation and the like are avoided by controlling the moisture content of the veneer in the technological process, the strength of the veneer is kept, on the basis, the veneer is coated with modified resin with modified PAE as amain raw material, a vacuum impregnation technology is combined, and thus the impact strength, the size stability and the waterproofness of the veneer are improved; a specific compound dye composed of a hindered amine light stabilizer and a bisazo acid dye is combined with a vertical dip dyeing process, so that the dyeing effect is attractive and lasting, meanwhile, the modified resin isolates the environment, and the color fastness of the veneer is further improved.

A detergent composition comprising a copolymer and at least one component selected from surface active agents and fabric softening compounds, the copolymer comprising at least one dye binding monomer and at least one anionic or nonionic monomer, with the proviso that the copolymer is not poly(1-vinylpyrrolidone-co-acrylic acid). Also a method of mitigating dye transfer in a detergent composition in the presence of typically encountered soils is provided. A dye binding copolymer comprising at least one anionic monomer selected from acrylic acid, methacrylic acid, vinyl sulphonic acid, itaconic acid, octanoic acid, hexanoic, hydroxy acetic acid or mixtures thereof or at least one nonionic monomer selected from polyethylene glycols and at least one dye binding monomer selected from vinyl imidazole, methyl vinyl imidazole, vinyl pyridine-N-oxide, vinyl pyrrolidone and mixtures thereof is also disclosed.

The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.

The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.

Protein scaffolds from tobacco mosaic virus coat protein modified to incorporate polyhistidine can bind to a metal or a dye while having improved self-assembly characteristics. The scaffold can take the form of tubes or disks, and can further be formed into dual plasmonic ring resonators. Such self-assembled structures provide useful optical properties.

The invention relates to an asymmetric metal complex black dye and a preparation method thereof. Specifically, the asymmetric metal complex black dye has a structure represented by the following formula (1). The dye combines the advantages of acid dyes and reactive dyes, and is suitable for printing dyeing of fiber materials containing hydroxyl or amino groups. The dyeing process is simple, stableand reliable, and the dyeing effect is excellent. The material dyed by the dye has good fastness to wet and fastness to daylight.

The invention is directed to binary oligonucleotide probes for nucleic analysis, which probes can be made of DNA or RNA that recognize nucleic acid analytes (both DNA and RNA) with unprecedented high selectivity under mild conditions and are highly sensitive to single nucleotide mismatches (SNP single nucleotide polymorphisms) without PCR amplification. In one group, the binary probes indicate that they have hybridized to a particular nucleic analyte by binding to a molecular beacon that gives off a fluorescent signal. A second group of binary probes bind to a dye such as malachite green, where upon hybridization to analyte the fluorescence of the dye increases dramatically and is easily detected and measured. The new binary probes require only about five minutes at room temperature to generate a detectable signal.

The invention relates to the technical field of dye preparation data processing, in particular to a liquid disperse dye suitable for high-temperature and high-pressure dyeing and a preparation method thereof.The dye comprises a composite dye matrix, a dispersion stabilizing system and a solvent medium, disperse black dye is formed by compounding disperse purple 93, disperse orange 288 and disperse blue 291 in the ratio of 1, and the disperse black dye is a liquid disperse dye suitable for high-temperature and high-pressure dyeing. And a nano-scale dispersion system is constructed by combining the synergistic effect of hexadecylamine / octadecylamine polyoxyethylene ether and lauryl amine polyoxyethylene ether. According to the preparation method, on the basis of a dynamic behavior prediction model, micelle formation dynamic data in a premixing stage, viscosity and particle size associated data in a shearing stage and thixotropic index change data in a homogenizing stage are integrated, and a surfactant proportion optimization instruction is generated; according to the invention, the stability of the dispersoid structure is controlled, the dyeing uniformity and color fastness are improved, and the wastewater COD discharge load and the production cost are reduced.

The invention discloses a method for quickly detecting white spirit based on a nano-material organic dye colorimetric sensor array. The method comprises the following steps of introducing six nano-materials and three dyes to construct a nano-material / dye composite colorimetric sensor array, and extracting change values (delta RGB) of array points RGB before and after reaction as detection signals; and processing the data by utilizing pattern recognition and a radial basis function neural network so as to explore the performance of the array detection white spirit. Compared with the prior art, the method has high recognition and detection capacity for different white spirits, combines a nano material with a traditional dye, improves recognition sensitivity of the array, reduces the number of array points, is simple in data processing, has the advantages of simple operation, short test time, high detection efficiency, good sensitivity and the like, and is suitable for popularization and application. The method has a good application prospect in the aspect of on-site real-time detection of white spirit.

The invention discloses a near-infrared fluorescent probe compound taking an N-pyridine oxide derivative as a recognition group as well as preparation and application of the near-infrared fluorescent probe compound. The preparation method specifically comprises the following steps: firstly, oxidizing a pyridine heterocyclic compound into an N-pyridine-2-formaldehyde oxide derivative, and taking the N-pyridine-2-formaldehyde oxide derivative as a recognition group; finally, enabling rhodamine B hydrazide to react with the N-pyridine oxide derivative in an organic solvent, so as to obtain the N-pyridine oxide-rhodamine B near-infrared fluorescent probe. The N-pyridine oxide-rhodamine B type near-infrared fluorescent probe can detect divalent palladium ions with high sensitivity and high selectivity, can realize colorimetric and fluorescent dual-mode detection of the divalent palladium ions in the environment, and can also be used for rapid detection of the divalent palladium ions in cells. Besides, the applied N-pyridine oxide derivative is a new recognition group which is easily available in initial raw material and easy to prepare, and has a wide application prospect in the aspect of detecting other heavy metal ions after being combined with other common dyes.

The invention discloses a method for improving dyeing performance of a real silk fabric based on enzymatic modification; serine hydroxymethyltransferase is used for catalyzing glycine in protein fibermacromolecules to be converted into serine, the number of the serine in real silk fiber is increased, phosphorylation of the serine and threonine residues in the real silk fiber is catalyzed by protein kinase A, the cationic dye binding effect is enhanced, and the dyeing performance of the real silk fabric is improved. The method specifically comprises the following steps: carrying out enzymaticconversion of amino acids in real silk; and (2) catalyzing the phosphorylation by the protein kinase A. Compared with the modification and improvement of the electronegativity of the surface of the real silk fiber by applying a chemical method, the method has the advantages of being high in enzyme catalysis efficiency, mild in reaction conditions and obvious in dyeing performance improvement.

A surgical instrument wrap having at least one or a plurality of fibrous layers that cooperate with at least one agent or dye layer that is generally not visible until the at least one or plurality of fibrous layers is damaged or disrupted. The at least one diffusible agent or dye layer diffuses or wicks into the disrupted or damaged area. A fluorescent dye, a reactive dye, a conjugant dye or an absorption wavelength of approximately 200-400 nanometers in one embodiment and an emission wavelength in the visible spectrum so that when the surgical wrap is damage or disrupted and then exposed to, for example, a fluorescent or ultraviolet light source, the agent or dye fluoresces to provide an indication to the user that damage to the wrap has occurred. The user can presume that the items or instruments in the surgical tray may be contaminated.

A rapid and facile design strategy to create a highly complex optical tag with programmable, multimodal photoluminescent properties is described. This is achieved via intrinsic and biomolecule-fluorophore hidden signatures. As a first covert feature of the tag, an intricate novel heterometallic near-infrared (NIR) emitting mesoporous metal-organic framework (MOF) was synthesized comprising homometallic hexanuclear clusters based on Nd and Yb. To generate controlled, multimodal, and tailorable emission with difficult to counterfeit features, the NIR emissive MOF was post-synthetically modified via a fluorescent biomolecule labeling design strategy. The surface attachment of several distinct fluorophores, including the simultaneous attachment of up to three distinct fluorescently labeled DNA oligos was demonstrated, with excitation and emission properties across the visible spectrum (480-800 nm). The DNA inclusion as a secondary covert element in the tag was demonstrated via detection of SYBR Gold dye association.

The invention discloses a hairpin template for detecting Bst DNA polymerase activity and application, a kit and a method thereof, and relates to the technical field of enzyme activity detection, and the nucleotide sequence of the hairpin template is as shown in SEQ ID No.1. The invention provides a specific hairpin template. The hairpin template has a stem-loop structure, is in a single-chain closed state when not amplified, is less combined with an embedded fluorescent nucleic acid dye, and has a lower background signal; when the Bst DNA polymerase is added, the enzyme has 5 '-> 3' DNA polymerase activity and strong strand displacement activity, the hairpin template can be self-triggered to amplify under the condition of no primer, the hairpin template is expanded and copied to form double-stranded DNA under the action of the Bst DNA polymerase, and the embedded fluorescent nucleic acid dye can be specifically combined with the double-stranded DNA to enhance a fluorescent signal of a system. The activity of the Bst DNA polymerase can be calculated according to the relationship between the double-stranded DNA and the fluorescence signal intensity.

Method for producing a color-stable or color-resistant image (10) in a surface (21) of a consumer article (20), in particular a sanitary article, such as a shower tray, bathtub, washbasin, toilet lid, seat ring, floor or wall panel, apron for bathtubs, bathroom furniture or household article, such as a chair, table, furniture, for example a sofa, the method comprising the following steps: - Providing (100) the surface (21), wherein the surface (21) comprises or is formed from a polymer material (22), in particular mineral casting, the polymer material (22) comprises at least one polymer (23) and one filler (24), and the filler (24) comprises silicon dioxide; - providing (200) a dye (30) for the image (10) to be produced; - combining (300) the surface (21) and the dye (30); and - heating (400) the dye (30), whereby the dye (30) diffuses into the surface (21) by sublimation and the image (10) is formed.

In the present invention, a region corresponding to the inside of a structure to be formed is irradiated with: polymerization promoting light (L1) for promoting the polymerization of a monomer (M) stored in a container (11) together with a dye; and binding promoting light (L3) for promoting the binding of the pigment to the monomer (M). The number of times of each point is irradiated with the polymerization promoting light (L1) is set according to the two-dimensional thickness distribution of the structure to be formed, and the energy of the binding promoting light (L3) applied to each point is set according to the two-dimensional refractive index distribution of the structure to be formed.

The invention discloses a cotton fiber direct dye less-water dyeing method based on an electrostatic spinning technology, and belongs to the technical field of textile chemical industry. The traditional cotton fiber dyeing mainly adopts a water bath dipping dyeing method which can generate a large amount of dyeing wastewater, but the method is a green and environment-friendly dyeing technology, and the adopted dyeing process comprises the following steps: by taking a PVA spinning solution as a carrier, depositing micro-nano fibers carrying a cationic modifier polyepichlorohydrin amide and a dye on a cotton fiber net, and carrying out dyeing on the micro-nano fibers to obtain a dyed cotton fiber net; according to the method, colored micro-nano fibers are loaded accurately, then boiling is carried out, a cationic modifier can be released to chemically react with cotton fibers, cationic modification is effectively carried out on the cotton fibers, and binding sites of the cotton fibers and dye are increased; meanwhile, the direct dye is rapidly dyed and fixed by virtue of the directness of the direct dye and the electrostatic attraction between the direct dye and the modified cotton fiber, so that synchronous coloring and ecological fixation are realized. The process can basically realize zero discharge of printing and dyeing wastewater.

A compound in which a xanthene colorant is bonded to an optical absorber (Dye) with optical absorption in the wavelength range of 350 to 700 nm.

A method for detecting microprotein in near-infrared region based on dye binding principle includes adding the aqueous solution of protein in bottle with incremental concentrations, adding acidic buffer solution, diluting with water, adding cyanine solution, standing and scanning absorption spectrum or measuring the increment of light absorption degree. Its advantages are high sensitivity and less interference.

A single stranded nucleic acid biosensor for 2′, 3′-cGAMP is provided. The single stranded nucleic acid may include a 2′, 3′-cGAMP-binding riboswitch domain comprising a transducer stem and a dye-binding aptamer domain that is operably connected to the transducer stem. A 2′, 3′-cGAMP-binding riboswitch domain. The dye-binding aptamer domain can be a Spinach2 aptamer. The 2′, 3′-cGAMP biosensor may further include a signaling chromophore specifically bound to the Spinach2 aptamer domain, where the sensor is configured to fluorescently activate the signaling chromophore upon specific binding of 2′, 3′-cGAMP to the 2′, 3′-cGAMP-binding riboswitch domain. Also provided are methods in which the subject 2′, 3′-cGAMP biosensors find use including methods for determining the level of cGAS activity in a sample or a cell. Nucleic acid constructs and host cells including the same are also provided.

Embodiments of the disclosure include methods and compositions related to use of compound comprising a particular peptide linked to a dye. In specific embodiments, the peptide DIRG is linked to the BODIPY dye. In specific embodiments, the compositions are utilized for treatment of neurological conditions and secondary pathologies related therewith, such as spinal cord injury and Alzheimer's Disease, as examples.

The present invention provides a preparation method of a composite material of polymerized hydroxy zinc modified kaolin, belonging to the technical field of composite materials. In the present invention, zinc ions polymerize with the hydroxyl groups of organic alkanolamines under alkaline conditions to form stable polymers; then, through stirring, the polymerized hydroxy zinc in the suspension is stably combined with kaolin through electrostatic and hydrogen bond interactions, etc., to obtain a polymerized hydroxy zinc modified kaolin adsorbent material. The polymerized hydroxy zinc modified kaolin adsorbent material prepared by the present invention has a large number of hydroxyl groups on its surface, which can be attracted and combined with triphenylmethane dyes through electrostatic adsorption; it can also be combined with triphenylmethane dyes through physical adsorption; the zinc ions on the polymerized hydroxy zinc itself have empty orbitals in the d electron orbitals, which can form stable coordination bonds with the N atoms on the triphenylmethane dye molecules; therefore, the polymerized hydroxy zinc modified kaolin adsorbent material prepared by the present invention can be used to treat wastewater containing triphenylmethane dyes.

The present invention relates to an erythropoietin-containing solution preparation containing a poloxamer and having a pH of 6.5 to 7.5. The present invention also relates to a method for quantifying a protein in a trace amount, the method including the following steps: binding a protein sample to a high-intensity fluorescent dye; separating a desired analyte from the obtained sample by an appropriate separation means; and quantifying the desired analyte and converting the amount of the analyte into the amount of the protein.