Method for determining microprotein in near infrared wavelength zone based on dye binding principle

A near-infrared, wavelength region technology, applied in the field of protein determination, can solve the problem of no substantial improvement in sensitivity, and achieve the effects of high sensitivity, large molar absorption coefficient, and significant enhancement of absorbance

Inactive Publication Date: 2003-03-12
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Numerous methods have also been published for protein quantitation using dye-conjugated methods, but with no substantial improvement in sensitivity over Coomassie Brilliant Blue

Method used

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  • Method for determining microprotein in near infrared wavelength zone based on dye binding principle
  • Method for determining microprotein in near infrared wavelength zone based on dye binding principle
  • Method for determining microprotein in near infrared wavelength zone based on dye binding principle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] An example of experimental operation under optimized conditions is given below.

[0036] 20μg / mL HSA water soluble

Liquid consumption (mL)

0.00

0.05

0.10

0.20

0.40

0.60

0.80

1.00

1.20

pH 2.6 Glycine - Salt

Acid buffer solution dosage

(mL)

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

Deionized redistilled water

Dilute to full scale (10.0mL)

1.0×10 -4 mol / L Cyanine

Solution consumption (mL)

0.00

0.30

0.30

0.30

0.30

0.30

0.30

0.30

0.30

HSA final concentration (ng / mL)

0

100

200

400

800

1200

1600

2000

2400

Absorbance

0.00

0

0.02

3

0.06

5

0.17

0...

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Abstract

A method for detecting microprotein in near-infrared region based on dye binding principle includes adding the aqueous solution of protein in bottle with incremental concentrations, adding acidic buffer solution, diluting with water, adding cyanine solution, standing and scanning absorption spectrum or measuring the increment of light absorption degree. Its advantages are high sensitivity and less interference.

Description

(1) Technical field [0001] The invention relates to a method for measuring protein, in particular to a method for measuring trace protein in the near-infrared wavelength region based on the principle of dye combination. (2) Background technology [0002] Cyanine dyes belong to a class of compounds. Cyanine dyes were first used as photosensitizers in the photographic chemical industry, or as energy conversion and storage materials, such as pigments for solar cells or laser dyes. In recent years, it has been found that this type of compound has long-wave fluorescence (600-1000nm) emission, so it is gradually used in fluorescence analysis, combined with laser-induced fluorescence (Laser-induced-fluorescence, LIF) technology and capillary electrophoresis (Caterpillar electroporesis, CE) technology or chromatographic separation technology, This kind of compound has been used for the sequencing of nucleic acid (Shealy, D.B.et al.Anal.Chem., 1995,67,247; Shealy, D.B.et al.Appl.Spec...

Claims

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Application Information

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IPC IPC(8): G01N21/3577G01N21/359G01N33/52
Inventor 郑洪李东辉毛玉霞
Owner XIAMEN UNIV
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