Method for measuring lysine in corn through near infrared spectrum

A near-infrared spectroscopy and lysine technology, which is applied in the field of measuring lysine in corn by near-infrared spectroscopy, can solve the problems of complex, time-consuming, and high cost of chemical analysis.

Inactive Publication Date: 2011-09-07
HENAN ACAD OF AGRI SCI
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Problems solved by technology

Among them, the determination process of the chemical analysis method is complicated, expensive and time-consuming; the amino acid analyzer method cannot be produced in China, and the imported equipment is expensive, and only a few specialized quality analysis units have it. Lysine content is not wi

Method used

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  • Method for measuring lysine in corn through near infrared spectrum
  • Method for measuring lysine in corn through near infrared spectrum
  • Method for measuring lysine in corn through near infrared spectrum

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1 A method for measuring lysine in corn by near-infrared spectroscopy, comprising the following steps:

[0022] (1) Determination of sample chemical value, spectrum collection: select 38 representative corn samples, analyze the chemical value of the lysine content of each sample, and scan and collect the near-infrared spectrum of each sample, using the diffuse reflectance integrating sphere attachment, The scanning range is 10000~4000cm -1 , the number of scans is 33, and the resolution is 8cm -1 , the standard ceramic sheet is used as a reference; the measured near-infrared diffuse reflectance spectrum, such as figure 1 shown;

[0023] (2) Elimination of outliers: Spectral and chemical value outlier (outlier) tests use two statistics, the spectral influence value Leverage and the chemical value error Residual, respectively. The calculation formula of the influence value is:

[0024] Leverage=z i T (Z T Z) -1

[0025] where z is the score matrix of p...

Embodiment 2

[0027] Example 2 Analysis Model Verification

[0028] (1) Sample processing

[0029] Test 1: Mix Zhengdan 2501 and Zhengdan 2503 samples evenly, take out 4 parts at random, 900g / part, and use 4 methods to measure the lysine content.

[0030] Test 2: Add industrial L-Lysine according to 0.1% and 0.2% of the mass of Zhengdan 2503 sample (passed through a 100-mesh sieve), mix well, and use Zhengdan 2503 sample without L-Lysine as a control, a total of 3 treatments , each treatment set 3 replicates. The content of lysine was determined by ninhydrin chromogenic method and dye binding method respectively.

[0031] (2) Lysine determination method

[0032] Dye binding method: carried out in the Agricultural Products Quality Supervision, Inspection and Testing Center (Zhengzhou) of the Ministry of Agriculture. Among them, the first test was completed by the staff of the inspection and testing center; the second test of adding industrial L-Lysine was jointly completed by the experim...

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Abstract

The invention relates to a method for measuring lysine in corn through a near infrared spectrum. The method comprises the following steps of: testing a spectrum, rejecting an abnormal value, establishing an analysis model and the like. By the method, the lysine content can be rapidly and nondestructively measured without pretreatment, and the method is particularly suitable for measuring the lysine content of low-generation materials in the process of breeding high-quality protein corn; compared with lysine content measured results in a dye binding method (DBL), a ninhydrin development process and an amino acid analyzer method, the lysine content measured result in the method verifies the feasibility of a method for measuring the corn through the near infrared spectrum, particularly lysine of the low-generation materials in the process of breeding the high-quality protein corn; moreover, the method is rapid, reliable and practical, and has certain reference value for selecting a method for measuring the lysine content in the process of breeding the high-quality protein corn.

Description

technical field [0001] The invention relates to an analysis and test method, in particular to a method for measuring lysine in corn by using near-infrared spectroscopy. Background technique [0002] Lysine is one of the eight essential amino acids that humans and animals need but cannot synthesize by themselves. The lack of this amino acid will cause metabolic disorders in the body and cause certain physiological functional diseases. Lysine is not high or lacking in most grains and is called the first limiting amino acid. Increasing the lysine content in cereal grains has become one of the main directions of breeding. Therefore, the determination of lysine content in cereal grains requires a fast, simple and accurate determination method suitable for screening low-generation materials. Since 1963, high-quality protein corn was discovered by Mertz and others in the United States o 2 Genes can significantly increase the lysine content of corn kernels; in 1969, Paze et al. f...

Claims

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Application Information

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IPC IPC(8): G01N21/25
Inventor 铁双贵岳润清齐建双王延召朱卫红卢彩霞
Owner HENAN ACAD OF AGRI SCI
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