34 results about "Aminoacid analyzer" patented technology

The invention discloses a breeding method and a preparation method of a new variety of health-care boxthorn leaf tea. A squashing technique is adopted for cytological observation; an over-parent heterosis method and an over-contrast dominant method are utilized to determine the growth advantages of the leaf area, the leaf thickness and the stem diameter of a mutant, the length of an edible part and the fresh weight of tender stems and leaves; a region production testing method is adopted to determine the yield performance of the new variety; a disease index method is used for identification of disease resistance; a semimicro kjeldahl method is adopted to determine the content of crude protein; a Soxhlet extraction method is utilized to determine the content of crude fat; an atomic absorption method is used for determining the contents of calcium, potassium, magnesium, sodium, phosphorus, copper, iron and the like; a fluorescence spectrophotometry is adopted to determine the content of selenium; an L-8500A amino acid analyzer is adopted to determine the content of amino acid; an ultrasonic extraction method is utilized to determine the contents of lycium barbarum polysaccharide and flavone; a spectrophotometer is used for determining the content of glycine betaine; the bred multiploid new variety has strong growth vigor, stable hereditary, super high yield, strong root rot resistance, high nutrition and good medicine quality, and the produced boxthorn leaf tea has high quality.

The invention belongs to the field of analytical chemistry, which relates to a method for determining nutrients, and discloses a method for simultaneously determining active peptide and free amino acid. By adjusting the composition of a buffer, elution process and elution temperature during an amino acid analysis determinator standard determination process, a plurality of amino acid peaks and active peptide peaks are separated, and an amino acid automatic analyzer can perform one-step determination on the free amino acid and the active peptide, a method for preparing the amino acid and peptide simultaneously is established, and the disadvantages that the active peptide and a plurality of free amino acids cannot be separated, and the time consuming for testing is long by a traditional amino acid analyzer method are overcome.

The invention discloses a method for analysis and determination by measuring the content of amino acids and proteins in food and feed. The content of proteins is measured by a kjeldah method and the content of various free and hydrolytic amino acids and the total content of amino acids are measured by an amino acid analyzer; a lot of samples are analyzed fundamentally to form a database and a judgment system; and each measured result is analyzed and compared with system data to judge whether fake proteins are adulterated in the food and the feed. By the method, a precise judgment can be made that components of non-proteins or fake proteins are adulterated in the sample only if one or more of the following measured data is abnormal: content of non-proteins, content of free amino acids, content of characteristic amino acids, percentage between the total content of amino acids and the proteins, the ratio of each component of the amino acids to the total content of the amino acids and the like, so that the quality safety of the food and the feed are ensured.

The invention relates to a culture method for improving the mycosporine-like amino acid content in porphyra umbilicalis. The method is characterized by including the following steps that 1, the vigorously growing porphyra umbilicalis is collected, screened and cleaned, is subjected to irradiation with ultraviolet rays with the wavelength of 254 nm for 3-10 minutes for sterilization and serves as a culture object; 2, the porphyra umbilicalis obtained in the step 1 is put in a piping type algae cultivation pool for seawater culture, the temperature is controlled to 20-35 DEG C, the pH is controlled to 5.6-6.4, multiple light sources consisting of an ultraviolet light lamp, a purple light lamp, a green light lamp and a yellow light lamp is adopted to perform continuous irradiation culture for 3-5 cycles, air is pumped in the culture process to keep the porphyra umbilicalis in a suspended state, possibly existing hybrid algae is periodically observed and removed to make the porphyra umbilicalis grow continuously, collection and air-drying are performed after the length of upright branches reaches 4-5 cm, and an amino acid analyzer and UHPLC are adopted to detect the MAAs content of the dried porphyra umbilicalis. The culture method is low in production cost, and the MAAs content of the cultured porphyra umbilicalis is increased remarkably.

Disclosed is a method for determining the content of tranexamic acid using an amino acid analyzer, and a method for determining the content of tranexamic acid by using ion-exchange chromatographic separation and a standard curve method. The chromatographic conditions are 4μm prewash column and 8μm analysis column, and the ion exchange resin is Biochrom30+ special resin for high-efficiency amino acid automatic analyzer; detection wavelength is 570nm; pump flow rate is 35ml / h; gradient elution program: 0~1min buffer 1, 1~ 2min buffer 2, 2-20min buffer 5, 20-21.5min buffer 6, 21.5-23.5min buffer 1; column temperature 44°C; reaction temperature 135°C; injection volume 20μl. The amino acid analyzer used in the determination of tranexamic acid content has the characteristics of commercialization and high degree of automation. The method for determining the content of tranexamic acid with an amino acid analyzer has the advantages of simple operation, strong standardization, high sensitivity, rapidity and high efficiency.

The invention discloses a method for measuring free ammonia in compound amino acid injection, the method comprising the following steps: providing an amino acid analyzer and a chromatographic column; preparing a buffer solution for separation and analysis; preparing a derivatization reaction reagent; the flow rate of the mobile phase is 0.40 ml / min, the flow rate of the derivatizing reagent is 0.35ml / min, the column temperature is 57°C, the reactor temperature is 135°C, the detection wavelength is 570nm, the injection volume is 5μl, and the gradient is eluted; the preparation of the test solution; the preparation of the reference solution Formulation and chromatographic determination. Wherein, during chromatographic determination, calculate the concentration of free ammonia in the test solution with the peak area according to the external standard method, and calculate the content of free ammonia in the compound amino acid injection according to the preparation method of the test solution. It also relates to the compound amino acid injection determined by the method of the invention. The method of the present invention exhibits excellent technical effects as described in the description, such as rapidity, simplicity, sensitivity and stability.

The invention discloses a method for detecting sulfur-containing amino acid. The method comprises the following steps of: oxidizing cystine and methionine and preparing cysteic acid and metson by acid hydrolysis; adding a derivatization reagent of 2,4-dinitrofluorobenzene to the cysteic acid and the metson for derivatization reaction to generate products having the function of ultraviolet absorption; and performing highly efficient liquid phase chromatographic analysis of the derivatives. The method has the advantages that: the amino acid analyzer with high price is unnecessary; the operationis simple; the detection cost is low; and the accuracy and the sensitivity are high.

The invention relates to a method for building a konjak finger print; the method adopting a high efficiency liquid phase chromatography includes the following steps: (a) the preparation of a sample: konjak tissues are taken for deactivation of enzymes, drying and trituration and then weighed in a test tube, and the constant volume and filter of the konjak tissues are made by purified water, and the sample undergoes the pre column derivation according to the analysis method for plant amide acids; (b) chromatogram conditions: the high efficiency liquid phase chromatograph or the amino acid analyzer is used, and the chromatographic column uses the octadecylsilane chemically bonded silica as fillers; (c) the detection: the sampling solution is precisely sucked and injected in the high efficiency liquid phase chromatograph, and the finger print is detected and obtained according to the high efficiency liquid phase chromatography. By analyzing the relation between characteristic peaks in the finger print and the breed, the quality, the resistance, the output and the growth state, etc. of the konjak, the invention can provide technical directions for scientific efforts such as the collection and classification of the konjak resource, the breeding selection of new breeds and the research of novel amino acid pesticides for controlling konjak diseases, etc.

The invention relates to a method for utilizing free amino acid spectroscopic data for identifying the authenticity of a maca product and determining the content of raw maca in the maca product. The method comprises the main steps of 1, manufacturing a mixed maca product; 2, manufacturing a detection sample; 3, adopting an amino acid analyzer for determining free amino acid spectroscopic data in pure maca, fake maca and mixed maca; 4, analyzing and processing the free amino acid data. According to the method, preprocessing is easy and convenient, maca authenticity identification and product maca content determination can be achieved rapidly, and the method is convenient to implement and practical. The prediction error of the method is smaller than 10%, and the detection requirement for the maca product can be better met. At present, no determination method for the content of maca in the maca product exists in China, and the method can be used for quality control in the industrial field of maca and products thereof.

The invention belongs to the field of analytical chemistry, which relates to a method for determining nutrients, and discloses a method for simultaneously determining active peptide and free amino acid. By adjusting the composition of a buffer, elution process and elution temperature during an amino acid analysis determinator standard determination process, a plurality of amino acid peaks and active peptide peaks are separated, and an amino acid automatic analyzer can perform one-step determination on the free amino acid and the active peptide, a method for preparing the amino acid and peptide simultaneously is established, and the disadvantages that the active peptide and a plurality of free amino acids cannot be separated, and the time consuming for testing is long by a traditional amino acid analyzer method are overcome.

The invention relates to a method for screening of methionine producing bacteria. A method in the prior art usually comprises the steps of transferring single colonies appearing on a flat into a shake flask, performing culturing for some time, and analyzing whether methionine is contained or not in fermentation liquid by methods of a spectroscopical photometric method, a chromatography or amino acid analyzer and the like. The method in the prior art is time-consuming. According to the method provided by the invention, the technological processes are simplified; after the colonies grow in plates and are cultured for some time, a series of sodium nitroprusside reagents are directly added to the surfaces of the colonies according to quantity, and after a reaction, whether the color is red is observed, so as to determine whether the colonies can produce methionine. According to the method disclosed by the invention, the screening time for methionine producing bacteria is shortened, and the screening efficiency is improved.

The invention discloses a method for identifying fermented type and formulated type yogurt beverages. Yogurt beverage is precipitated by sulfosalicylic acid, the content of free amino acids in liquid supernatant is measured by an amino acid analyzer, and the content ratios of four characteristic amino acids-alanine, valine, lysine and proline of total free amino acids are judged to establish the amino acid analysis method for identifying fermented type and formulated type yogurt beverages. According to the method, the similarity of the fermented type and formulated type yogurt beverages in the aspects of mouthfeel and physical behavior is avoided, the difference of the contents of the free amino acids of the fermented type and formulated type yogurt beverages is fully utilized, and a problem that the fermented type and formulated type yogurt beverages cannot be rapidly identified is solved; and the identification method can be operated simply and conveniently and has high accuracy and good application prospect.

The invention discloses a method for measuring main stream smoke of cigarettes by using an amino acid analyzer. The analytical method which is used for measuring ammonia in the main stream smoke of cigarettes by using the amino acid analyzer and a post column derivation device thereof is established according to the selection specificity of color development reaction of ammonia and ninhydrin and by using the design principle of the amino acid analyzer and improving an amino acid analysis method, and the problem of sodion interference puzzled by ion chromatography is successfully avoided; and the method disclosed by the invention is simple, convenient, rapid and accurate and is suitable for actual analysis and detection of a cigarette smoke sample.