The invention relates to a culture method for improving the mycosporine-like
amino acid content in
porphyra umbilicalis. The method is characterized by including the following steps that 1, the vigorously growing
porphyra umbilicalis is collected, screened and cleaned, is subjected to
irradiation with
ultraviolet rays with the
wavelength of 254 nm for 3-10 minutes for sterilization and serves as a culture object; 2, the
porphyra umbilicalis obtained in the step 1 is put in a
piping type
algae cultivation
pool for
seawater culture, the temperature is controlled to 20-35 DEG C, the pH is controlled to 5.6-6.4, multiple light sources consisting of an
ultraviolet light lamp, a purple light lamp, a
green light lamp and a yellow light lamp is adopted to perform
continuous irradiation culture for 3-5 cycles, air is pumped in the culture process to keep the porphyra umbilicalis in a suspended state, possibly existing
hybrid algae is periodically observed and removed to make the porphyra umbilicalis grow continuously, collection and air-
drying are performed after the length of upright branches reaches 4-5 cm, and an
amino acid analyzer and UHPLC are adopted to detect the MAAs content of the dried porphyra umbilicalis. The culture method is low in production cost, and the MAAs content of the cultured porphyra umbilicalis is increased remarkably.