A kind of culture method that can improve mycosporine-like amino acid content in umbilical laver

A culture method and amino acid technology are applied in the culture field that can increase the content of bacteriocin-like amino acids in umbilical laver, can solve the problems of uncontrollable conditions, difficulty in amplification and high investment cost, and achieve the effects of low production cost and increased content

Active Publication Date: 2020-10-02
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation methods and devices for seaweed cultivation disclosed in the above-mentioned patented technologies all have disadvantages such as complicated process, difficulty in scaling up, difficult control of conditions, high labor intensity, and high investment cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of culture method that can improve mycosporin-like amino acid accumulation in umbilical laver adopts the following steps:

[0018] (1) Collect 29°32′~31°04′N, 121°31′~123°25′E, which grow sessilely in the intertidal zone and offshore area of ​​Zhoushan Islands, and screen the algae to be thick, shiny, and without obvious necrosis Tissues of navel laver were washed twice with boiled fresh water and irradiated with ultraviolet light at a wavelength of 254 nm for 3 min to further remove possible miscellaneous algae and native microorganisms. The algae cultivation pool was placed in a cool and ventilated place, and the seawater filtered and sterilized under the condition of 10 μm was passed through.

[0019] (2) Evenly disperse the umbilical laver to be cultivated in the algae culture tank, turn on the air blower at the bottom of the culture tank to pump air to keep the laver in a suspended state, control the water temperature of the culture tank to 20 ℃, pH to 5.6, ...

Embodiment 2

[0021] A kind of culture method that can improve mycosporin-like amino acid accumulation in umbilical laver adopts the following steps:

[0022] (1) Collect 29°32′~31°04′N, 121°31′~123°25′E, which grow sessilely in the intertidal zone and offshore area of ​​Zhoushan Islands, and screen the algae to be thick, shiny, and without obvious necrosis Tissues of navel laver were washed twice with boiled fresh water and irradiated with ultraviolet light at a wavelength of 254 nm for 5 min to further remove possible miscellaneous algae and native microorganisms. The algae cultivation pool was placed in a cool and ventilated place, and the seawater filtered and sterilized under the condition of 10 μm was passed through.

[0023] (2) Evenly disperse the umbilical laver to be cultivated in the algae culture tank, turn on the air blower at the bottom of the culture tank to pump air to keep the laver in a suspended state, control the water temperature of the culture tank to 25 °C, pH to 5.9,...

Embodiment 3

[0025] A kind of culture method that can improve mycosporin-like amino acid accumulation in umbilical laver adopts the following steps:

[0026] (1) Collect 29°32′~31°04′N, 121°31′~123°25′E, which grow sessilely in the intertidal zone and offshore area of ​​Zhoushan Islands, and screen the algae to be thick, shiny, and without obvious necrosis Tissues of umbilical laver were washed twice with boiled fresh water and irradiated with ultraviolet light at a wavelength of 254 nm for 7 minutes to further remove possible miscellaneous algae and native microorganisms. The algae cultivation pool was placed in a cool and ventilated place, and the seawater filtered and sterilized under the condition of 10 μm was passed through.

[0027] (2) Evenly disperse the umbilical laver to be cultivated in the algae culture tank, turn on the air blower at the bottom of the culture tank to pump air to keep the laver in a suspended state, control the water temperature of the culture tank to 30 °C, pH...

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Abstract

The invention relates to a culture method for improving the mycosporine-like amino acid content in porphyra umbilicalis. The method is characterized by including the following steps that 1, the vigorously growing porphyra umbilicalis is collected, screened and cleaned, is subjected to irradiation with ultraviolet rays with the wavelength of 254 nm for 3-10 minutes for sterilization and serves as a culture object; 2, the porphyra umbilicalis obtained in the step 1 is put in a piping type algae cultivation pool for seawater culture, the temperature is controlled to 20-35 DEG C, the pH is controlled to 5.6-6.4, multiple light sources consisting of an ultraviolet light lamp, a purple light lamp, a green light lamp and a yellow light lamp is adopted to perform continuous irradiation culture for 3-5 cycles, air is pumped in the culture process to keep the porphyra umbilicalis in a suspended state, possibly existing hybrid algae is periodically observed and removed to make the porphyra umbilicalis grow continuously, collection and air-drying are performed after the length of upright branches reaches 4-5 cm, and an amino acid analyzer and UHPLC are adopted to detect the MAAs content of the dried porphyra umbilicalis. The culture method is low in production cost, and the MAAs content of the cultured porphyra umbilicalis is increased remarkably.

Description

technical field [0001] The invention relates to the cultivation of seaweed, in particular to a cultivation method capable of increasing the content of mycosporine-like amino acids in navel laver. Background technique [0002] Umbilical seaweed (Porphyra suborbiculata Kjellm) is a marine red algae (Red algae), containing up to 29-35% protein, of which a kind of amino acid active substance after protein hydrolysis, mycosporine-like amino acid is also called trityl imidazole amino acid ( Mycosporine-like amino acids (MAAs for short) is a class of water-soluble, small molecule (<400Da) compounds with ultraviolet absorption properties. The absorption peak of MAAs compounds is between 310 and 360 nm, so it has the ability to absorb ultraviolet radiation and can reduce the damage caused by ultraviolet radiation to cells. In addition, MAAs also has the function of inhibiting peroxides, active oxygen and free radicals, and has a good antioxidant effect when applied in cosmetics. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/02A01G7/04
CPCA01G7/045A01G33/00Y02P60/14
Inventor 周浩淼毕天宇刘丽杨盼盼毕永贤蒋丽刚
Owner PROYA COSMETICS
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