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Method for preparing active peptide from waste fermented grains

A technology of active peptides and white wine, which is applied in the direction of fermentation, can solve the problems that have not been reported, and achieve the effect of simple process, small molecular weight and high purity

Inactive Publication Date: 2017-03-22
SHAOYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Searching Chinese patent literature, there are more than 144 applications on the reuse of discarded grains, such as CN201110119777.6 The resourceful and harmless treatment method of the largest solid waste of traditional liquor, CN201210252105.7 The resourceful treatment of fly maggots Luzhou-flavor type Method for discarding distilled grains, CN201110119843.X method for producing natural flavors for vinegar by combining solid-liquid distilled grains with distilled wine, etc., but there is no research on the distilled grains and their enzymatic hydrolyzates having biological activities such as ACE inhibition and anti-oxidation see the report

Method used

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  • Method for preparing active peptide from waste fermented grains
  • Method for preparing active peptide from waste fermented grains
  • Method for preparing active peptide from waste fermented grains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Raw material pretreatment: Take the distilled grains and add 8 times the volume of water to fully stir for 5 minutes, use gauze to filter out the suspended rice husk, and the remaining mixture is ultrasonically crushed (20 KHz) 5 times every 2 minutes for a total of 10 minutes, and tissue Homogenize for 20 min.

[0020] 2) Enzymatic hydrolysis of waste protein: adjust the pH value of the homogenate in step (1) to 5.5 with 1 mol / L hydrochloric acid, preheat at 50 °C for 5 minutes, add 6% (w / w) papain, and perform enzymatic hydrolysis at 50 °C for 4 h, inactivate the enzyme at 80°C for 10 min to terminate the reaction, and perform ultrafiltration of the final enzymatic hydrolysis product on a 3 kDa membrane, collect the extramembrane liquid and freeze-dry to obtain the active peptide, measure the molecular weight distribution ratio and biological activity; the yield of the active peptide is 11- 12%;

[0021] 3) Purification of active peptides: Dissolve the above activ...

Embodiment 2

[0023] 1) Raw material pretreatment: Take the distilled grains and add 8 times the volume of water to stir for 5 minutes, filter the suspended rice husks with gauze, and ultrasonically break the remaining mixture (25 KHz) 10 times every 2 minutes for a total of 20 minutes, and organize Homogenize for 25 min.

[0024] 2) Enzymatic hydrolysis of discarded waste protein: adjust the pH value of the homogenate to 6.0 with 1 mol / L hydrochloric acid, preheat at 50 °C for 5 min, add 6% (w / w) papain, enzymatically hydrolyze at 60 °C for 4 h, at 80 °C The reaction was terminated by inactivating the enzyme at ℃ for 15 minutes, and the final enzymatic hydrolysis product was subjected to 3 kDa membrane ultrafiltration, and the extramembrane fluid was collected to obtain the active peptide after freeze-drying, and the molecular weight distribution ratio and biological activity were determined; the yield of the active peptide was 11-12%;

[0025] 3) Purification of active peptides: Dissolve ...

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Abstract

The invention discloses a method for preparing active peptide from waste fermented grains. The method comprises steps of pre-processing raw materials, enzymatically hydrolyzing waste fermented grain protein and purifying the active peptide; the active peptide, which is low in molecular weight and high in activity, is prepared by virtue of a selective enzymatic hydrolysis technology and 3kDa membrane ultrafiltration; the active peptide is highly purified by virtue of gel filtration chromatography and high performance liquid chromatography two-step separation technology; the ACE inhibitory activity IC50 of the active peptide is 1.14 mg / ml; DPPH scavenging capacity EC50 is 1.42 mg / ml, ABTS scavenging capacity EC50 is 2.56 mg / ml, reducing power (A700nm=0.5) is 375.48 [mu]g / ml, and purity reaches 92.1% or above. The method provides by the invention adopts the waste fermented grains of Daqu liquor, Xiaoqu liquor, mould bran liquor and the like as the raw materials; the method is applicable to industrial production and strong in operability, and a target object is low in molecular weight and high in purity; and the method is capable of promoting the development and the utilization of by-product resources of liquor-making industry in China and achieving the efficient preparation of the active peptide.

Description

technical field [0001] The invention relates to a method for preparing active peptides by using liquor discarded grains, and belongs to the technical field of deep processing of liquor brewing by-products. Background technique [0002] Liquor distilled grains are the residues of sorghum, corn and other raw materials through solid-state fermentation and distillation to extract alcohol and aroma components. The national annual output of distilled spirits distilled grains is more than 25 million tons (Li Xinshe, Lu Bushi. The effect of Daqu distilled grains instead of rice bran to produce small koji Research[J]. Winemaking, 2006, 33(4): 65-66). The water content and acidity of liquor discarded grains are relatively high, which is easy to be corrupted and difficult to store. If it is directly discarded or burned, it will cause serious environmental pollution. The crude protein content of distilled spirits accounts for 14-22%, the starch content accounts for 10-13%, the fat cont...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/16C07K1/14
CPCC12P21/06C07K1/14C07K1/16
Inventor 伍强余有贵
Owner SHAOYANG UNIV
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