60 results about "Dpph scavenging" patented technology

The invention discloses a method for preparing antioxidant peptides from oats. Alcalase is determined to serve as the enzyme for preparation through investigating the antioxidant activities and yields of the products obtained by carrying out enzymolysis on the oat bran proteins with different proteases. Reduction of the enzymolysis speed of the Alcalase toward the oat bran proteins is determined to be mainly caused by substrate consumption and enzyme activity loss through investigating the enzymolysis speed of the Alcalase toward the oat bran proteins. Meanwhile, an enzymatic membrane reactorsystem is determined to have good retention effect on the Alcalase through studying the impact of the enzymatic membrane reactor system on the enzyme activity. The activity loss of the Alcalase is mainly caused by the damage to the enzyme in the operation process of a peristaltic pump and the adsorption of an ultrafiltration membrane toward the enzyme. By utilizing Design Expert 6.0.5 to design the response surface analysis experiment with four factors and three levels, the optimum process conditions under which the antioxidant peptides from oats can be prepared by utilizing the enzymatic membrane reactor are as follows: the substrate concentration is 3%; the enzyme addition is 3.2%; the feed flow rate is 45L / h; the operation pressure is 0.07MPa; the temperature is 55 DEG C; and the pH value is 7.3. Through verification, the DPPH scavenging rate of the products under the conditions can reach 57.39%.

The invention discloses an exopolysaccharide-producing space lactobacillus plantarum SS18‑33 and its application in improving biological antioxidant activity. The preservation number of the space Lactobacillus plantarum SS18-33 disclosed by the present invention in the General Microorganism Center of China Microbiological Culture Collection Management Committee is CGMCC No.15151. The exopolysaccharide produced by the space lactobacillus plantarum SS18-33 of the present invention has DPPH scavenging ability, superoxide anion free radical (O 2 ‑ ·) scavenging ability, for Fe 2+ Chelating ability and the total reducing ability or total antioxidant ability to block the formation of peroxides by providing hydrogen atoms; in addition, the strain also has the characteristics of gastrointestinal adverse environment tolerance, which contributes to the improvement of biological antioxidant activity of exopolysaccharides The application in provides a practical basis. The invention fills up the research blank of space food microbial engineering bacteria.