Method for preparing antioxidant peptides from oats by utilizing enzymatic membrane reactor

Abstract

The invention discloses a method for preparing antioxidant peptides from oats. Alcalase is determined to serve as the enzyme for preparation through investigating the antioxidant activities and yields of the products obtained by carrying out enzymolysis on the oat bran proteins with different proteases. Reduction of the enzymolysis speed of the Alcalase toward the oat bran proteins is determined to be mainly caused by substrate consumption and enzyme activity loss through investigating the enzymolysis speed of the Alcalase toward the oat bran proteins. Meanwhile, an enzymatic membrane reactorsystem is determined to have good retention effect on the Alcalase through studying the impact of the enzymatic membrane reactor system on the enzyme activity. The activity loss of the Alcalase is mainly caused by the damage to the enzyme in the operation process of a peristaltic pump and the adsorption of an ultrafiltration membrane toward the enzyme. By utilizing Design Expert 6.0.5 to design the response surface analysis experiment with four factors and three levels, the optimum process conditions under which the antioxidant peptides from oats can be prepared by utilizing the enzymatic membrane reactor are as follows: the substrate concentration is 3%; the enzyme addition is 3.2%; the feed flow rate is 45L / h; the operation pressure is 0.07MPa; the temperature is 55 DEG C; and the pH value is 7.3. Through verification, the DPPH scavenging rate of the products under the conditions can reach 57.39%.

Description

technical field

[0001] The invention relates to a method for preparing oat antioxidant peptide by using an enzyme membrane reactor. Background technique

[0002] Bioactive peptide is a general term for different peptides composed of 20 natural amino acids in different compositions and arrangements, ranging from dipeptides to complex linear and circular structures. It is the most complex multifunctional compound derived from proteins. It has human metabolism and Physiological adjustment function, high food safety, is the most popular research topic in the international food industry [1] . With the development of biological science and technology and the strengthening of people's awareness of health care, bioactive peptides have become the main development trend of the current pharmaceutical and health care products industry. In recent years, antioxidants have been vigorously developed at home and abroad, and their uses are becoming more and more extensive. They are not only...

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