Technique for preparing squid skin collagen peptide through enzymic method

A technology of enzymatic preparation and process method, applied in the direction of fermentation, etc., can solve the problems of low utilization rate of raw materials of aquatic products, environmental pollution, waste of resources, etc., and achieve the effect of ideal conversion rate of polypeptide, high theoretical guidance significance, and high utilization value

Inactive Publication Date: 2016-06-08
ZHEJIANG YUXIANG BIOLOGICAL SCI & TECH
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  • Application Information

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Problems solved by technology

However, the raw material utilization rate of aquatic products is low, and a large amount of leftover and waste materials of aquatic products are processed into feed without intensive processing, resulting in a great waste of resources and, more seriously, environmental pollution
At the same time, the low utilization rate of raw materials for aquatic product processing and the scarcity of high value-added products have become the most prominent problems faced by the aquatic product processing industry, which seriously limits the extension and sustainable development of the aquatic product processing industry chain.

Method used

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  • Technique for preparing squid skin collagen peptide through enzymic method
  • Technique for preparing squid skin collagen peptide through enzymic method
  • Technique for preparing squid skin collagen peptide through enzymic method

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Embodiment Construction

[0015] The present invention will be further described below in conjunction with the drawings and specific embodiments, but the protection scope of the present invention is not limited thereto.

[0016] Materials and Reagents

[0017] Squid skin: purchased from Zhoushan squid processing factory, washed and drained, and stored in a -20°C refrigerator.

[0018] Alkaline protease (2.4AU / g), flavor protease (500LAPU / g): Novozymes; papain (200000U / g): Guangxi Nanning Pangbo Bioengineering Co., Ltd.; Gly-Gly-Tyr-Arg: Sigma ; Diphenylpicryl radical (DPPH), copper sulfate, concentrated sulfuric acid, potassium sulfate, ninhydrin, fructose, glycine, trichloroacetic acid, absolute ethanol, sodium hydroxide, concentrated hydrochloric acid, etc. are analytically pure.

[0019] Instruments and Equipment

[0020] HH2 digital display constant temperature water bath: Guohua Electric Co., Ltd.; RE-52 rotary evaporator: Shanghai Jiapeng Technology Co., Ltd.; UV-2550 ultraviolet-visible spectr...

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Abstract

The invention relates to a technique for preparing squid skin collagen peptide through an enzymic method. The technique includes: pre-treating squid skin which is a raw material, hydrolyzing through alkaline protease, and continuing hydrolysis through composite protease to obtain collagen hydrolysate, wherein the composite protease is composed of papain and flavorzyme. The collagen peptide prepared by the technique is ideal in hydrolysis degree, DPPH removal rate and polypeptide conversion rate, molecular weight of 97.63% of collagen peptide is less than 2000 Da, and the collagen peptide is high in quality and utilization value. The technique has high theoretical guiding significance in subsequent further separation and purification and application of the squid skin collagen peptide.

Description

technical field [0001] The invention relates to a process for preparing squid skin collagen peptide, in particular to a process for preparing squid skin collagen peptide by enzymatic method. Background technique [0002] With the rapid development of my country's aquatic product processing industry, a large amount of waste is produced during the processing process, including fish skin, fish scales and fish bones, etc., accounting for about 50-70% of the total weight of fish. However, the raw material utilization rate of aquatic products is low, and a large amount of leftover and waste materials of aquatic products are processed into feed without intensive processing, resulting in a great waste of resources and, more seriously, environmental pollution. At the same time, the low utilization rate of raw materials for aquatic product processing and the scarcity of high value-added products have become the most prominent problems faced by the aquatic product processing industry, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 刘振锋吕卫金黄小鸣戴圣佳刘东红陈士国叶兴乾
Owner ZHEJIANG YUXIANG BIOLOGICAL SCI & TECH
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