Technique for preparing squid skin collagen peptide through enzymic method
A technology of enzymatic preparation and process method, applied in the direction of fermentation, etc., can solve the problems of low utilization rate of raw materials of aquatic products, environmental pollution, waste of resources, etc., and achieve the effect of ideal conversion rate of polypeptide, high theoretical guidance significance, and high utilization value
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[0015] The present invention will be further described below in conjunction with the drawings and specific embodiments, but the protection scope of the present invention is not limited thereto.
[0016] Materials and Reagents
[0017] Squid skin: purchased from Zhoushan squid processing factory, washed and drained, and stored in a -20°C refrigerator.
[0018] Alkaline protease (2.4AU / g), flavor protease (500LAPU / g): Novozymes; papain (200000U / g): Guangxi Nanning Pangbo Bioengineering Co., Ltd.; Gly-Gly-Tyr-Arg: Sigma ; Diphenylpicryl radical (DPPH), copper sulfate, concentrated sulfuric acid, potassium sulfate, ninhydrin, fructose, glycine, trichloroacetic acid, absolute ethanol, sodium hydroxide, concentrated hydrochloric acid, etc. are analytically pure.
[0019] Instruments and Equipment
[0020] HH2 digital display constant temperature water bath: Guohua Electric Co., Ltd.; RE-52 rotary evaporator: Shanghai Jiapeng Technology Co., Ltd.; UV-2550 ultraviolet-visible spectr...
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