Snake skin collagen peptide and preparation and application thereof

A collagen peptide and collagen technology, which is applied in the field of protein active peptides, can solve the problems of low antioxidant activity and low yield of active active peptides from snakeskin active peptides, and achieve less impurities, fewer ineffective components, and antioxidant properties. Good results

Pending Publication Date: 2019-04-16
克劳丽化妆品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to overcome the enzymolysis of snakeskin collagen peptides from fresh snakeskin tissue in the prior art, resulting in low effective active peptide yield and low antioxidant activity of snakeskin active peptides. High technical defect, thereby provide a kind of snake skin collagen peptide that adopts snake skin collagen as raw material, purity is high, the snake skin collagen peptide yield rate that obtains is high, antioxidant performance is good snake skin collagen peptide and preparation method thereof

Method used

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  • Snake skin collagen peptide and preparation and application thereof
  • Snake skin collagen peptide and preparation and application thereof

Examples

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Effect test

Embodiment 1

[0042] The present embodiment provides a kind of concrete implementation of snakeskin collagen protein extraction, comprises the following steps:

[0043] Blood and bacteria removal: Cut Chinese water snake skin into sections, soak in 4% NaCl solution for 4 hours, replace with new NaCl solution, repeat 4 times, and drain;

[0044] Impurity removal: use NaCO with a mass fraction of 10% 3 The solution was soaked and stirred for 16 hours, then washed 3 times with pure water;

[0045] Pulverizing: the snakeskin is freeze-dried with a vacuum freeze dryer, and after freeze-drying, it is pulverized with a pulverizer to obtain snakeskin powder;

[0046] Degreasing: add n-hexane to degrease the snakeskin powder, supplemented with ultrasound for 2 hours, the frequency is 100KHz, then stir for 12 hours, drain the n-hexane to obtain the pretreated snakeskin powder, wherein the ratio of n-hexane and snakeskin powder is 10ml : 1g;

[0047] Extraction: Dissolve the degreased snake skin po...

Embodiment 2

[0051] The present embodiment provides a kind of concrete implementation of snakeskin collagen protein extraction, comprises the following steps:

[0052] Blood and bacteria removal: cut the snake skin into sections, add 4% NaCl solution to soak for 4 hours, replace with new NaCl solution, repeat 5 times, and drain;

[0053] Impurity removal: use NaCO with a mass fraction of 10% 3 The solution was soaked and stirred for 12 hours, then washed 5 times with pure water;

[0054] Pulverization: freeze-dry the snakeskin with a vacuum freeze dryer, and pulverize it with a pulverizer after freeze-drying to obtain snakeskin powder;

[0055] Degreasing: add n-hexane to degrease the snakeskin powder, supplemented with ultrasound for 3 hours, the ultrasonic frequency is 5KHz, then stir for 16 hours, drain the n-hexane to obtain the pretreated snakeskin powder, wherein the ratio of n-hexane and snakeskin is 15ml : 1g;

[0056] Extraction: Dissolve the degreased snakeskin powder in 1mol / ...

Embodiment 3

[0060] This example provides a specific embodiment of snake skin collagen peptide.

[0061] The snakeskin collagen freeze-dried powder described in Example 1 is dissolved in 25 times of water, which is the snakeskin collagen sample liquid; the pH value of the snakeskin collagen sample liquid is adjusted to 7.5, and collagenase is added, and the collagenase The ratio to the snake skin collagen freeze-dried powder is 150000U: 1g, carry out enzymatic digestion at 48°C for 2 hours; inactivate the enzyme activity at 95°C for 20 minutes, cool the enzymatic cutting solution to room temperature, and use sodium hydroxide solution to dissolve Adjust the pH value of the enzyme cutting solution to 7 to obtain the enzymolysis solution of snakeskin collagen peptide; dialyze the enzymolysis solution through a 20KDa ultrafiltration membrane and a 100Da dialysis bag to obtain a snakeskin collagen peptide solution of 100Da-20KDa. The collagen peptide solution is subjected to vacuum freeze-dryin...

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Abstract

The invention relates to the technical field of active protein peptides, in particular to a snake skin collagen peptide and preparation and application thereof. According to the snake skin collagen peptide, purified snake skin collagen is adopted as an enzyme cutting raw material; through single enzyme digestion and combined multi-enzyme digestion, the snake skin collagen peptide with the molecular weight of 100 Da-20 KDa is obtained. The snake skin collagen peptide has high antioxidant activity, the DPPH clearance rate of the snake skin collagen peptide with the concentration of 1 mg / ml reaches 49.36%, and the clearance rate of superoxide anions reaches 51.12%. A provided preparation method of the snake skin collagen peptide has the advantages of being simple in enzymolysis process, highin enzyme digestion efficiency and short in production cycle, the yield of the snake skin peptide is higher than 52%, and the antioxidation activity is high. When the snake skin peptide is applied tothe cosmetic field, a cosmetic capable of resisting skin aging is obtained and has remarkable antioxidation activity.

Description

technical field [0001] The invention relates to the technical field of protein active peptides, in particular to a snakeskin collagen peptide, its preparation and application, and anti-aging cosmetics using the snakeskin collagen peptide. Background technique [0002] Peptides are a class of biologically active substances that involve various cellular functions in living organisms. They participate in the regulation of various complex physiological processes in the human body. They have the characteristics of high activity, strong affinity and high safety. Peptide molecules at the micron level can easily The surface layer of skin peptide directly enters the deep layer of skin peptide, and is quickly absorbed by the body to play a role. Modern pharmacological studies have shown that some active peptides not only have antioxidant activity, but also can effectively promote the growth and proliferation of human skin fibroblasts, especially by increasing the activity of skin fibr...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06A61K8/65A61Q19/08A61K38/39A61P17/18
CPCA61K8/65A61K38/39A61P17/18A61Q19/08C07K14/78C12P21/06
Inventor 周华锋蔡衡刘新旗周建青杜兆阳
Owner 克劳丽化妆品股份有限公司
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