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Method for preparing dendrobium officinale kimura et migo oligosaccharide having in vitro anti-oxidation activity

A technology of antioxidant activity and Dendrobium candidum, applied in fermentation and other directions, can solve the problems of slow development speed, lack of deep technological innovation, difficulty in adapting to the development of Dendrobium candidum planting industry, etc., and achieve the effect of strong implementation and simple method

Active Publication Date: 2017-10-03
江西九草铁皮石斛科技协同创新有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development speed of the intensive processing industry of Dendrobium candidum in my country is quite slow, and the lack of in-depth technological innovation has made it difficult to adapt to the development of modern Dendrobium candidum planting industry.

Method used

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  • Method for preparing dendrobium officinale kimura et migo oligosaccharide having in vitro anti-oxidation activity
  • Method for preparing dendrobium officinale kimura et migo oligosaccharide having in vitro anti-oxidation activity
  • Method for preparing dendrobium officinale kimura et migo oligosaccharide having in vitro anti-oxidation activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The fresh strips of Dendrobium officinale were placed in an oven for drying, then crushed with a universal grinder and passed through a 60-mesh separating sieve for later use.

[0030] Take 1g of Dendrobium candidum dry powder and put it in a 100mL Erlenmeyer flask, add 50mL of phosphate buffer and mix well, add 12U / mL enzyme preparation for enzymolysis, the enzymolysis temperature is 40°C, the enzymolysis time is 8h, after enzymolysis Place the samples in boiling water for 5 minutes to inactivate the enzyme activity, centrifuge the enzymatic hydrolysis product to get the supernatant, concentrate the supernatant to 1 mL, and finally measure the in vitro antioxidant activity of each sample.

[0031] Wherein, the pH of the phosphate buffer solution is 6.0; the enzyme preparation is a complex of β-mannanase and cellulase, and the ratio is 2:1.

[0032] After enzymatic hydrolysis under the above conditions, the obtained results are as follows:

[0033] The DPPH clearance r...

Embodiment 2

[0038] The fresh strips of Dendrobium officinale were placed in an oven for drying, then crushed with a universal grinder and passed through a 60-mesh separating sieve for later use.

[0039] Take 1g of Dendrobium officinale dry powder and put it in a 100mL Erlenmeyer flask, add 50mL of phosphate buffer and mix well, add 5U / mL enzyme preparation for enzymolysis, the enzymolysis temperature is 30°C, and the enzymolysis time is 12h. After enzymolysis Place the samples in boiling water for 5 minutes to inactivate the enzyme activity, centrifuge the enzymatic hydrolysis product to get the supernatant, concentrate the supernatant to 1 mL, and finally measure the in vitro antioxidant activity of each sample.

[0040] Wherein, the pH of the phosphate buffer solution is 7.0; the enzyme preparation is a single β-mannanase.

[0041] After enzymatic hydrolysis under the above conditions, the obtained results are as follows:

[0042] The DPPH clearance rate of the enzymolysis Dendrobium ...

Embodiment 3

[0044] The fresh strips of Dendrobium officinale were placed in an oven for drying, then crushed with a universal grinder and passed through a 60-mesh separating sieve for later use. Take 1g of Dendrobium officinale dry powder and put it in a 100mL Erlenmeyer flask, add 50mL of phosphate buffer and mix well, add a certain amount of enzyme preparation for enzymolysis, the enzymolysis temperature is 30°C, and the enzymolysis time is 12h. Place the sample in boiling water and cook for 5 minutes to inactivate the enzyme activity, centrifuge the enzymolysis product to get the supernatant, concentrate the supernatant and place it in about 1 mL.

[0045] Different enzymes were selected to hydrolyze Dendrobium officinale, and the in vitro antioxidant activity of the hydrolyzed products was determined. The results are shown in Table 1.

[0046] Table 1. Effects of different enzymes on the antioxidant activity of hydrolyzate of Dendrobium officinale

[0047]

[0048]The results show...

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Abstract

The present invention belongs to the field of processing of dendrobium officinale kimura et migo, and particularly relates to a method for preparing dendrobium officinale kimura et migo oligosaccharide having an in vitro anti-oxidation activity. According to the present invention, the dendrobium officinale kimura et migo oligosaccharide is prepared by using the enzymolysis method, the method is simple and has the strong practicality, and the prepared dendrobium officinale kimura et migo oligosaccharide has the DPPH removal rate of 23.7% and the total anti-oxidation activity of 0.65 U / mL.

Description

technical field [0001] The invention belongs to the field of dendrobium candidum processing, and in particular relates to a method for preparing dendrobium candidum oligosaccharides with in vitro antioxidant activity. Background technique [0002] Dendrobium officinale is a precious medicinal herb of the family Dendrobium genus Aerophytes. It has the functions of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat, moistening the lungs and relieving cough, prolonging life, etc. It can also enhance immunity, anti-oxidation, lower blood sugar, lower blood fat, and prevent cancer. Anti-cancer and other miraculous effects. The traditional processing of Dendrobium candidum is still based on the extensive processing of Dendrobium fresh strips, Tiepi Fengdou, and Dendrobium superfine powder. The development speed of the deep processing industry of Dendrobium candidum in my country is quite slow, and the lack of deep innovation of technology has m...

Claims

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Application Information

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IPC IPC(8): C12P19/14
CPCC12P19/14
Inventor 张秀清王国鑫陈洲孙君社裴海生何彬王民敬
Owner 江西九草铁皮石斛科技协同创新有限公司
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