Method for preparing dendrobium officinale kimura et migo oligosaccharide having in vitro anti-oxidation activity
A technology of antioxidant activity and Dendrobium candidum, applied in fermentation and other directions, can solve the problems of slow development speed, lack of deep technological innovation, difficulty in adapting to the development of Dendrobium candidum planting industry, etc., and achieve the effect of strong implementation and simple method
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Embodiment 1
[0029] The fresh strips of Dendrobium officinale were placed in an oven for drying, then crushed with a universal grinder and passed through a 60-mesh separating sieve for later use.
[0030] Take 1g of Dendrobium candidum dry powder and put it in a 100mL Erlenmeyer flask, add 50mL of phosphate buffer and mix well, add 12U / mL enzyme preparation for enzymolysis, the enzymolysis temperature is 40°C, the enzymolysis time is 8h, after enzymolysis Place the samples in boiling water for 5 minutes to inactivate the enzyme activity, centrifuge the enzymatic hydrolysis product to get the supernatant, concentrate the supernatant to 1 mL, and finally measure the in vitro antioxidant activity of each sample.
[0031] Wherein, the pH of the phosphate buffer solution is 6.0; the enzyme preparation is a complex of β-mannanase and cellulase, and the ratio is 2:1.
[0032] After enzymatic hydrolysis under the above conditions, the obtained results are as follows:
[0033] The DPPH clearance r...
Embodiment 2
[0038] The fresh strips of Dendrobium officinale were placed in an oven for drying, then crushed with a universal grinder and passed through a 60-mesh separating sieve for later use.
[0039] Take 1g of Dendrobium officinale dry powder and put it in a 100mL Erlenmeyer flask, add 50mL of phosphate buffer and mix well, add 5U / mL enzyme preparation for enzymolysis, the enzymolysis temperature is 30°C, and the enzymolysis time is 12h. After enzymolysis Place the samples in boiling water for 5 minutes to inactivate the enzyme activity, centrifuge the enzymatic hydrolysis product to get the supernatant, concentrate the supernatant to 1 mL, and finally measure the in vitro antioxidant activity of each sample.
[0040] Wherein, the pH of the phosphate buffer solution is 7.0; the enzyme preparation is a single β-mannanase.
[0041] After enzymatic hydrolysis under the above conditions, the obtained results are as follows:
[0042] The DPPH clearance rate of the enzymolysis Dendrobium ...
Embodiment 3
[0044] The fresh strips of Dendrobium officinale were placed in an oven for drying, then crushed with a universal grinder and passed through a 60-mesh separating sieve for later use. Take 1g of Dendrobium officinale dry powder and put it in a 100mL Erlenmeyer flask, add 50mL of phosphate buffer and mix well, add a certain amount of enzyme preparation for enzymolysis, the enzymolysis temperature is 30°C, and the enzymolysis time is 12h. Place the sample in boiling water and cook for 5 minutes to inactivate the enzyme activity, centrifuge the enzymolysis product to get the supernatant, concentrate the supernatant and place it in about 1 mL.
[0045] Different enzymes were selected to hydrolyze Dendrobium officinale, and the in vitro antioxidant activity of the hydrolyzed products was determined. The results are shown in Table 1.
[0046] Table 1. Effects of different enzymes on the antioxidant activity of hydrolyzate of Dendrobium officinale
[0047]
[0048]The results show...
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