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Quinoa peptide with whitening and antioxidant activity as well as preparation method and application thereof

A technology of antioxidant activity and quinoa peptide, which is applied in the preparation methods of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problems of insufficient enzymatic hydrolysis, whitening activity, low antioxidant activity, and active Problems such as large molecular weight of peptides, to achieve the effect of simple method, high whitening and antioxidant activity, and high content of small molecular peptides

Active Publication Date: 2021-09-03
科丽思化妆品(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the preparation method of quinoa peptide in the related art is mainly through a single enzymatic hydrolysis method, such as adding pepsin, trypsin or papain alone, to obtain active peptides from quinoa, but this single enzymatic hydrolysis method is easy Insufficient enzymatic hydrolysis, the obtained active peptide has a large molecular weight, has the disadvantages of whitening activity and low antioxidant activity

Method used

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  • Quinoa peptide with whitening and antioxidant activity as well as preparation method and application thereof
  • Quinoa peptide with whitening and antioxidant activity as well as preparation method and application thereof
  • Quinoa peptide with whitening and antioxidant activity as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] A preparation method of quinoa peptide with whitening and antioxidant activity, the specific preparation steps are as follows:

[0092] S1. Saponin removal treatment: wash the quinoa three times with warm water at 45°C to remove the saponins contained in the epidermis.

[0093] S2. Drying and crushing: drying the washed quinoa at 50° C. for 12 hours, pulverizing with a pulverizer, and passing through a 50-mesh sieve to obtain quinoa powder.

[0094] S3. Degreasing treatment: mix quinoa flour and n-hexane with a mass ratio of 1:5, stir at room temperature for 2 hours, remove the quinoa flour grease, then perform suction filtration, collect the filter residue and dry to obtain defatted quinoa flour, and the filtrate is rotary evaporated to recover n-hexane to recycle.

[0095] S4. Quinoa protein extraction: mix defatted quinoa flour and deionized water at a mass ratio of 1:13, stir evenly, adjust the pH to 11 with 0.1mol / L HCL and 0.1mol / L NaOH, and heat to 40°C. Extrac...

Embodiment 2-22

[0099] A preparation method of quinoa peptide with whitening and antioxidative activity, the difference from Example 1 is that the graded enzymolysis conditions are different, specifically the addition of alkaline protease, papain or acid protease is different, the enzyme Solution pH, temperature or time are different, other steps and conditions are identical with embodiment 1.

[0100] The specific graded enzymatic hydrolysis condition parameters in Examples 1-22 and the antioxidant and whitening activity tests of quinoa peptides are included in Table 3.

[0101] Table 3 Example 1-22 different enzymatic hydrolysis conditions and quinoa peptide activity test results

[0102]

[0103]

[0104]

[0105]

[0106] As can be seen from the test results in Table 3, compared with Examples 1-3, the pH of alkaline protease is 9.3, 8.5, and 10 in turn, and the other parameters are the same, while the quinoa peptide prepared in Example 1 has free hydroxyl groups. The test res...

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Abstract

The invention relates to the technical field of plant extraction, and particularly discloses quinoa peptide with whitening and antioxidant activity as well as a preparation method and application thereof. Alkaline protease, papain and acid protease are adopted to form specific compound protease for performing graded enzymolysis on quinoa protein, wherein the alkaline protease is added for enzymolysis under certain pH and temperature conditions firstly, then the pH and temperature conditions are adjusted, the papain is added for enzymolysis, then the pH and temperature conditions are further adjusted, the acid protease is added for enzymolysis, and the quinoa peptide is obtained. The quinoa peptide disclosed by the invention is small in molecular weight, relatively high in hydroxyl radical scavenging rate, DPPH scavenging rate and tyrosinase inhibition rate, and has the effects of whitening and resisting oxidation. Therefore, the quinoa peptide prepared by the invention can be used in cosmetics, health care products or foods. The method disclosed by the invention is simple to operate, high in safety and easy to industrialize, and has important significance on fully utilizing quinoa resources and developing functional products.

Description

technical field [0001] The present application relates to the technical field of plant extraction, more specifically, it relates to a quinoa peptide with whitening and anti-oxidation activities, its preparation method and application. Background technique [0002] Quinoa belongs to Chenopodiaceae, an annual dicotyledonous plant. The appearance of the plant is white, milky yellow, purple red, and black. The colors of the seeds are mainly black, red, and white. Hard, small seeds, in the shape of small round pills. It is rich in plant protein. It has been reported that the protein content in quinoa grains is about 13-17%, some as high as 22%, and its protein content is generally better than that of ordinary cereals. In addition, it is rich in a variety of amino acids, including all 9 essential amino acids necessary for the human body; the content of total polyphenols, saponins, flavonoids and polysaccharides is also very rich. Quinoa is a whole grain, whole nutrition, complet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/415C07K1/14C07K1/34A23L33/18A61K8/64A61Q19/02A61Q19/08
CPCC12P21/06C07K14/415A23L33/18A61K8/645A61Q19/02A61Q19/08A61K2800/782A61K2800/805
Inventor 周海军
Owner 科丽思化妆品(上海)有限公司
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