The present patent application discloses a recombinant human collagen type I and its use. The amino acid sequence of the recombinant human collagen type I is shown in SEQ ID No: 2. The recombinant human collagen type I has good cellproliferation activity, cell migration activity, cell adhesion activity (all better than full-length recombinant human collagen type I or commercially available human collagen), and is easy to produce. The recombinant human collagen type I is expected to have the effects of whitening, moisturizing, soothing and repairing, and can be applied in the fields of cosmetics, external use drugs, skin care products, health care products, tissue engineering materials, medical devices and the like.