Anti-wrinkle firming face cream as well as preparation method and application thereof

Abstract

The invention provides an anti-wrinkle firming face cream and a preparation method and application thereof, and the anti-wrinkle firming face cream comprises the following components in percentage by mass: 4-20% of emollient, 2-9% of humectant, 0.5-3% of anti-wrinkle agent and the balance of solvent, the emollient comprises a plant extract; the plant extract comprises a citrus fruit extract, a burdock root extract, a phellinus linteus extract, a centella asiatica extract, a fig fruit extract, an apple fruit extract, a Kava pepper leaf / root / stem extract, a cocoa seed extract and a mussel extract; the humectant is prepared from glycerol, butanediol, betaine, sodium hyaluronate and trehalose; the anti-wrinkle agent is prepared from hydroxypropyl tetrahydropyrantriol. The anti-wrinkle and firming face cream provided by the invention is scientific in formula, and tests prove that the face cream has the effects of moisturizing, relieving, repairing, firming, resisting wrinkles, and being mild and non-irritating.

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, and in particular to an anti-wrinkle and firming facial cream and a preparation method and application thereof. Background Art

[0002] After undergoing skin aesthetic treatments such as laser, microneedle, and water light needle, the original barrier of the skin is changed during the operation, the skin is more fragile, and problems such as redness, sensitivity, and stinging will occur; the skin barrier function may be temporarily weakened after aesthetic treatment, and it will take some time to recover. In order to promote skin recovery and reduce complications, postoperative care is essential. The skin condition after aesthetic treatment is related to the individual's skin recovery ability. Usually, people who have undergone aesthetic treatment will use basic skin care products such as facial masks and creams to help the skin recover quickly. However, during the recovery period, irritating skin care products should be avoided to avoid increasing the burden on the skin. Currently, the facial cream products on the market pay little attention to the skin's elasticity, the fading and reduction of wrinkles, and rarely involve the repair issues after aesthetic treatment. Summary of the invention

[0003] In view of this, the purpose of the present invention is to provide an anti-wrinkle and firming facial cream and a preparation method and application thereof to solve the above problems.

[0004] The technical solution of the present invention is achieved in this way:

[0005] An anti-wrinkle and firming facial cream comprises the following components by mass percentage: 8% to 20% of an emollient, 2% to 9% of a moisturizer, 0.5% to 3% of an anti-wrinkle agent and the remainder of a solvent;

[0006] The emollient includes a plant extract;

[0007] The plant extract comprises the following raw materials: citrus (CITRUS RETICULATA) fruit extract, burdock (ARCTIUM LAPPA) root extract, phellinus linteus extract, centella asiatica (CENTELLA ASIATICA) extract, fig (FICUS CARICA) fruit extract, apple (PYRUS MALUS) fruit extract, kava pepper (PIPER METHYSTICUM) leaf / root / stem extract, cocoa (THEOBROMA CACAO) seed extract and mussel extract;

[0008] The moisturizing agent comprises the following raw materials: glycerin, butylene glycol, betaine, sodium hyaluronate and trehalose;

[0009] The anti-wrinkle agent includes hydroxypropyl tetrahydropyrantriol.

[0010] A further solution is that, by mass percentage, the following components are also included: 0.02% to 0.08% of a chelating agent, 0.5% to 3% of a surfactant, 0.3% to 3% of an antioxidant, 1% to 5% of an emulsifier, 0.2% to 2% of an emulsion stabilizer, and 1% to 3% of a thickener;

[0011] The emulsion stabilization comprises at least one of palmitic acid, stearic acid, cetearyl alcohol, cetyl alcohol or sodium stearoyl glutamate;

[0012] The emulsifier includes at least one of cetearyl glucoside, glyceryl stearate or PEG-75 stearate;

[0013] The thickener includes sodium polyacrylate and / or dextrin;

[0014] The antioxidant comprises at least one of tocopheryl acetate, tocopherol or p-hydroxyacetophenone;

[0015] The surfactant includes ceteth-20 and / or steareth-20;

[0016] The solvent includes water and / or 1,2-hexanediol;

[0017] The chelating agent includes disodium EDTA.

[0018] A further solution is that, calculated by mass percentage, the following components are included: 12% to 18% emollient, 2% to 6% humectant, 0.5% to 3% anti-wrinkle agent and the remainder solvent; the humectant includes 0.5% to 3% glycerol, 0.2% to 1% butylene glycol, 0.2% to 1% betaine, 0.5% to 2% sodium hyaluronate and 0.2% to 2% trehalose.

[0019] A further scheme is that the mass ratio of the citrus (CITRUS RETICULATA) fruit extract, burdock (ARCTIUMLAPPA) root extract, phylloxera (PHELLINUS LINTEUS) extract, Centella asiatica (CENTELLA ASIATICA) extract, fig (FICUS CARICA) fruit extract, apple (PYRUS MALUS) fruit extract, piper methystICUM leaf / root / stem extract, cocoa (THEOBROMA CACAO) seed extract and mussel extract is 1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2.

[0020] A further embodiment is that the emollient further comprises one or more of cyclopentasiloxane, mango (MANGIFERAINDICA) seed fat, bifida fermentation product filtrate, ethylhexylglycerin, hydrogenated lecithin, hydrolyzed hyaluronic acid, madecassoside, panthenol, caprylic / capric triglyceride, ceramide, raffinose, cholesterol, folic acid, soluble collagen, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-5, palmitoyl pentapeptide-4, sucrose stearate or superoxide dismutase.

[0021] A further solution is to add, by mass percentage, 0.2% to 2% of cyclopentasiloxane, 0.1% to 1.2% of mango (MANGIFERAINDICA) seed fat, 0.2% to 2% of bifida yeast fermentation product filtrate, 0.2% to 2% of ethylhexylglycerol, 0.2% to 2% of hydrogenated lecithin, 0.5% to 3% of hydrolyzed hyaluronic acid, 0.5% to 3% of madecassoside, 0.5% to 2% of panthenol, 0.2% to 2% of caprylic / capric triglyceride, 0.2% to 2% of ceramide, 0.2% to 2% of raffinose, 0.2% to 2% of cholesterol, 0.2% to 2% of folic acid, 0.2% to 2% of soluble collagen, 0.2% to 2% of dipeptide diaminobutyryl benzylamide diacetate, 0.2% to 2% of palmitoyl tripeptide-5, and 0.2% to 2% of palmitoyl pentapeptide-4. 0.2%~2%, sucrose stearate 0.2%~2% or superoxide dismutase 0.5%~2%.

[0022] The present invention provides a method for preparing the above-mentioned anti-wrinkle and firming cream, comprising the following steps: adding an emollient, a moisturizer and an anti-wrinkle agent into a solvent, and stirring at 400 to 800 rpm for 30 to 50 minutes.

[0023] The present invention provides the anti-wrinkle and firming facial cream for use in reducing the number and volume of under-eye wrinkles.

[0024] The present invention provides the above-mentioned anti-wrinkle and firming cream, which is used in improving wrinkles or fine lines, improving skin elasticity and firmness, or reducing skin hemoglobin after medical aesthetics.

[0025] A further embodiment is that the improvement of wrinkles or fine lines includes reducing the number, length or depth of wrinkles or fine lines, and the wrinkles or fine lines include application to crow's feet, nasolabial folds or forehead wrinkles.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The anti-wrinkle and firming cream provided by the present invention has a scientific formula. It has been verified by experiments that the cream has the effects of moisturizing, soothing, repairing, firming, anti-wrinkle, mild and non-irritating, and can effectively reduce the number and volume of under-eye wrinkles. In addition, the anti-wrinkle and firming cream of the present invention can enhance the skin absorption effect, fade and reduce wrinkles, improve skin elasticity and firmness, etc. It is especially suitable for people who have undergone medical aesthetics, has a soothing effect on damaged skin barriers, and improves the fragile state of the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a satisfaction chart after using the anti-wrinkle and firming cream of Example 1 for 28 days;

[0029] Figure 2 This is a satisfaction chart after using the anti-wrinkle and firming cream of Example 1 for 28 days;

[0030] Figure 3 This is a bar graph showing the crow's feet wrinkle depth test results before and after the use of the sample group of Test Example 2;

[0031] Figure 4 The bar graph of the crow's feet wrinkle length test results before and after the use of the sample group of Test Example 2;

[0032] Figure 5 This is a bar graph showing the results of the depth test of crow's feet fine lines before and after the use of the sample group of Test Example 2;

[0033] Figure 6 This is a bar graph showing the test results of the length of crow's feet fine lines before and after the use of the sample group of Test Example 2;

[0034] Figure 7 This is a bar graph of the nasolabial fold width test results before and after the use of the sample group of Test Example 2;

[0035] Figure 8 This is a bar graph showing the results of forehead wrinkle number detection before and after use of the sample group in Test Example 2;

[0036] Fig. 9 This is a bar graph of the forehead wrinkle length test results before and after the use of the sample group of Test Example 2;

[0037] Fig.10 This is a bar graph showing the results of the forehead wrinkle and fine lines test before and after the use of the sample group in Test Example 2;

[0038] Fig.11 This is a bar graph showing the results of forehead wrinkle and fine line length testing before and after the use of the sample group in Test Example 2;

[0039] Fig.12 This is a bar graph of the jawline angle test results before and after using the sample group of Test Example 2;

[0040] Fig.13This is a bar graph showing the test results of the skin elasticity coefficient R2 value of the sample group in Test Example 2 before and after use;

[0041] Fig.14 This is a bar graph of the skin firmness F4 value test results of the sample group of Test Example 2 before and after use;

[0042] Fig.15 This is a bar graph of the skin hemoglobin index test results before and after use of the sample group of Test Example 2;

[0043] Among them, T0 represents the initial value, T2 represents the use of the sample for 2 weeks, and T4 represents the use of the sample for 4 weeks; P>0.05 indicates no statistical difference (indicated by "ns"), P<0.05 indicates a significant difference (indicated by "*"), and P<0.01 indicates an extremely significant difference (indicated by "**"). DETAILED DESCRIPTION

[0044] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0045] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0046] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0047] Example 1

[0048] The formula of the anti-wrinkle firming cream is shown in Tables 1 and 2 below.

[0049] Table 1 Anti-wrinkle firming cream formula

[0050]

[0051]

[0052] Table 2 Dosage ratio of plant extracts

[0053]

[0054] The preparation method of the anti-wrinkle and firming cream is as follows: an emollient, a moisturizing agent, an anti-wrinkle agent, an emulsifier, an emulsion stabilizer, a thickener, a surfactant, a chelating agent, an antioxidant, a pH regulator and a preservative are added to a solvent, and the mixture is stirred at 600 to 800 rpm for 40 minutes to obtain the anti-wrinkle and firming cream.

[0055] Test Example 1

[0056] 1. Reference method: T / CAB 0152-2022 "Test methods for seven cosmetic effects: anti-wrinkle, firming, moisturizing, oil control, repairing, nourishing and soothing";

[0057] "Evaluation of Cosmetic Efficacy (X.III.)—Consumer Use Testing".

[0058] 2. Test sample: the anti-wrinkle and firming cream of Example 1;

[0059] How to use: Use every morning and evening after cleansing. Take an appropriate amount of this product on your fingertips or palms, and then gently and evenly massage on the facial skin;

[0060] Test area: face.

[0061] 3. Subjects: All informed consent must be signed before the start of the study;

[0062] 3.1 Inclusion criteria:

[0063] ① Chinese healthy male / female subjects with sensitive skin aged between 18 and 60 years old;

[0064] ②The TEWL value of the skin in the measurement area is greater than 10;

[0065] ③ There are visible fine lines under the eyes on the facial skin;

[0066] ④ No products with the same efficacy as the test product, such as repairing efficacy, were used in the month before the test;

[0067] ⑤ Willing not to use cosmetics, health products, drugs or other types of products with the same effects as the test product during the trial period, except for the test product;

[0068] ⑥Can read Chinese, understand it accurately, and have signed the informed consent form;

[0069] ⑦ Able to cooperate with and participate in the trial follow-up time, and promptly report any changes in their own health status or medication, and symptoms of adverse reactions.

[0070] 3.2 Exclusion criteria:

[0071] ① Women who are currently breastfeeding or pregnant, or women who plan to become pregnant in the next two months;

[0072] ② The test area has obvious sunburn, scars, pigmented nevi, excessive hair growth or other conditions that may affect the test evaluation results;

[0073] ③ Those with bacterial, viral or fungal infection on the skin of the test site;

[0074] ④People with chronic skin diseases (such as skin tumors, rosacea, eczema, lupus erythematosus, psoriasis, severe epidermal exfoliation, etc.);

[0075] ⑤ A history of immunosuppression or immunodeficiency (including HIV or AIDS), or current use of immunosuppressive drugs or radiation therapy;

[0076] ⑥ Those suffering from chronic diseases and endocrine diseases such as asthma, epilepsy, diabetes, hypertension, hyperthyroidism or hypothyroidism;

[0077] ⑦ Participate in other clinical trials within 3 months;

[0078] ⑧ Any medication that may affect skin condition or reaction, such as antihistamines, antibiotics, insulin, anti-inflammatory drugs, vitamin A, steroids, aspirin, thyroid drugs, etc., that has been used in the past 6 months or is currently used;

[0079] ⑨ The tested area has undergone medical cosmetic treatment within 6 months, such as laser treatment, chemical peeling and other cosmetic treatments, and minimally invasive cosmetic treatments such as Botox;

[0080] ⑩ Those who have been diagnosed with allergies or other known or suspected allergic reactions (systemic, inhalation or local), and have a history of sensitivity to the ingredients of the formula;

[0081] Other diseases or conditions that, according to the judgment of the trial institution, reduce the possibility of enrollment or complicate enrollment, such as frequent changes in work environment, unstable living environment, and other conditions that are likely to cause loss to follow-up: alcohol and / or psychoactive substance, drug abusers and addicts;

[0082] Those with a history of mental illness or unable to take care of themselves.

[0083] In addition, people who are judged to be unsuitable as subjects for this trial.

[0084] 3.3 Restrictions:

[0085] ①During the test, it is prohibited to use any items with the same function in the test area except the test sample;

[0086] ②During the test, frequent entry and exit of the constant temperature and humidity room is prohibited;

[0087] ③During the test, it is prohibited to cover or touch the test part during the test.

[0088] 3.4 Target number of cases:

[0089] It is planned to enroll 33 qualified subjects and ensure that more than 30 subjects will be selected in the end.

[0090] 3.5 Screening failure, subject dropout and replacement:

[0091] The subjects who were not included in the study were those who signed the informed consent but failed to be included in the study because they did not meet the inclusion / exclusion criteria.

[0092] If a subject leaves the study after being assigned to a group, it is considered to have dropped out. If a subject does not complete the study for any reason, it is considered to have dropped out and no additional enrollment will be made.

[0093] Shedding criteria:

[0094] Subject dropout consent: Subjects can withdraw at any time for any reason (in accordance with the Helsinki Declaration);

[0095] Adverse event / serious adverse event: any event that the investigator believes is detrimental to the subjects if the trial is continued;

[0096] Not following the protocol;

[0097] Subjects were lost to follow-up;

[0098] Other reasons: other reasons for subjects’ withdrawal;

[0099] → In other cases, the researcher believes that continued participation in the trial would be detrimental to the subject;

[0100] The CRF form must be filled out until the candidate fails the screening or is dropped out.

[0101] 4. Test equipment

[0102] Table 3

[0103] Serial number Test parameters Measurement method Measurement area 1 Transepidermal water loss Tewameter Face 2 Water content of skin stratum corneum Corneometer Face 3 Analysis of under eye wrinkles Primos image acquisition and analysis Under eye wrinkle area

[0104] 5. Test environment

[0105] Environmental requirements: Temperature: 21.0℃±1.0℃; Humidity: 50%±10%

[0106] 6. Testing process

[0107] 6.1 Experimental design

[0108] Self-controlled before-after test

[0109] 6.2 Use period of test samples

[0110] Use according to the instructions for 28 days (allow 2 days of error)

[0111] 7. Test result statistical method

[0112] The samples were compared before and after, and the above mean values ​​were analyzed for differences. When the two groups of data were normally distributed at the same time, the paired T test was used to analyze the differences between the two groups of data; when the two groups of data were not normally distributed at the same time, the rank sum test was used to analyze the differences between the two groups of data.

[0113] The transepidermal water loss (TEWL) of the skin is measured by Tewameter and is an important parameter reflecting the water loss and barrier function of the skin. The lower the measured value, the better the barrier function of the skin.

[0114] The moisture content of the stratum corneum of the skin is measured by a Corneometer and is an important parameter reflecting the moisture content of the skin. The higher the measured value, the higher the moisture content of the skin.

[0115] Wrinkle parameters are obtained by taking 3D images of the local skin at the corners of the eyes of the subjects with Primos CR and then analyzing them with professional analysis software. The number of wrinkles and wrinkle volume are the parameters that can better reflect the degree of wrinkles. The smaller the values ​​of these parameters, the better the wrinkle condition.

[0116] 8. Test results

[0117] (1) Subjects

[0118] Planned number of participants: 33

[0119] Number of participants: 32

[0120] Number of people who completed: 30

[0121] Number of analysts: 30

[0122] The age analysis of the subjects is shown in Table 4.

[0123] Table 4 Subjects' information

[0124]

[0125] 9. Test results

[0126] 9.1 Statistical table of transepidermal water loss rate of skin, see Table 5.

[0127] Table 5 Statistics of skin transepidermal water loss (unit: g / hm 2 )

[0128] time N Transepidermal water loss P-value 0d 30 15.52±1.17 0.000 14d 30 12.26±0.87 0.021 28d 30 11.52±0.75 0.002

[0129] 9.2 Analysis of differences in transepidermal water loss rate of skin, see Table 6.

[0130] Table 6 Analysis of differences in transepidermal water loss rate of skin (comparison before and after using samples)

[0131]

[0132]

[0133] It can be seen from Tables 5 and 6 that 14 days after using the samples, the transepidermal water loss rate of the sample areas of the 30 test subjects decreased by 21.02%, which is significantly different from that before using the samples.

[0134] 28 days after using the samples, the transepidermal water loss rate of the sample areas of the 30 test subjects decreased by 25.78%, which was significantly different from that before using the samples.

[0135] 9.3 Statistical table of moisture content of skin stratum corneum, see Table 7.

[0136] Table 5 Statistics of moisture content of skin stratum corneum (unit: cu)

[0137] time N Water content of skin stratum corneum P-value 0d 30 23.73±1.57 0.183 14d 30 29.12±1.71 0.200 28d 30 30.96±1.72 0.200

[0138] 9.4 Analysis of differences in moisture content in the stratum corneum of the skin, see Table 8.

[0139] Table 8 Analysis of differences in moisture content of the stratum corneum of the skin (comparison before and after using the sample)

[0140] time N Mean P-value Difference Analysis 14d 30 5.39 P<0.01 There is a significant difference 28d 30 7.24 P<0.001 There is a significant difference

[0141] It can be seen from Tables 7 and 8 that 14 days after using the samples, the moisture content of the stratum corneum of the skin in the sample areas of the 30 test subjects increased by 22.71%, which was significantly different from that before using the samples.

[0142] 28 days after using the samples, the moisture content of the stratum corneum of the skin in the sample areas of 30 subjects increased by 30.49%, which was significantly different from before using the samples.

[0143] 9.5 Statistical table of the number of wrinkles under the eyes, see Table 9.

[0144] Table 9 Statistics of the number of wrinkles under the eyes (unit: line)

[0145] time N Number of wrinkles under eyes P-value 0d 30 367.83±12.14 0.200 14d 30 222.93±9.61 0.200 28d 30 209.80±8.39 0.200

[0146] 9.6 Analysis of differences in the number of under-eye wrinkles, see Table 10.

[0147] Table 10 Analysis of differences in the number of wrinkles under the eyes (comparison before and after using the sample)

[0148]

[0149]

[0150] It can be seen from Tables 9 and 10 that 14 days after using the samples, the number of under-eye wrinkles in the sample area of ​​30 subjects decreased by 39.39%, which is significantly different from before using the samples.

[0151] 28 days after using the sample, the number of under-eye wrinkles in the sample area of ​​30 subjects decreased by 42.96%, which was significantly different from before using the sample.

[0152] 9.7 Statistics of under-eye wrinkle volume, see Table 11.

[0153] Table 11 Statistics of wrinkle volume under the eyes (unit: mm 3 )

[0154] time N Volume of wrinkles under eyes P-value 0d 30 12.15±0.61 0.200 14d 30 9.44±0.58 0.200 28d 30 9.04±0.54 0.200

[0155] 9.8 Analysis of differences in the volume of under-eye wrinkles, see Table 12.

[0156] Table 12 Analysis of differences in the volume of wrinkles under the eyes (comparison before and after using the sample)

[0157] time N Mean P-value Difference Analysis 14d 30 -2.71 P<0.001 There is a significant difference 28d 30 -3.11 P<0.001 There is a significant difference

[0158] It can be seen from Tables 11 and 12 that 14 days after using the samples, the volume of under-eye wrinkles in the sample area of ​​30 subjects decreased by 22.30%, which is significantly different from before using the samples.

[0159] 28 days after using the sample, the volume of under-eye wrinkles in the sample area of ​​30 subjects decreased by 25.63%, which was significantly different from before using the sample.

[0160] 10. Self-evaluation by the subjects

[0161]

[0162]

[0163]

[0164]

[0165] 11. Conclusion

[0166] 11.1 The results of the above clinical efficacy trials show that:

[0167] 1. After using the test product, the subjects' transepidermal water loss rate showed a significant downward trend at each follow-up point, indicating that under the test conditions, the test product has a repairing effect and can improve the skin barrier.

[0168] 2. After using the test product, the moisture content of the subjects' skin stratum corneum showed a significant upward trend at each follow-up point, indicating that under the test conditions, the test product has a moisturizing effect.

[0169] 3. After using the test product, the number and volume of the subjects' under-eye wrinkles showed a significant downward trend at each follow-up time point, indicating that under the test conditions, the test product has anti-wrinkle effects and can improve under-eye wrinkles.

[0170] 4. Under the experimental conditions, no adverse reactions were observed in the subjects.

[0171] 11.2 The product evaluation test was conducted through a consumer self-evaluation questionnaire survey. The results showed that:

[0172] 1. At the 28-day follow-up, after using the test product, 100.00% of the subjects felt that their facial skin was more hydrated and the test product had a moisturizing effect.

[0173] It indicates that under the test conditions, the test product has moisturizing effect.

[0174] 2. At the 28-day follow-up, after using the test product, 96.97% of the subjects felt that the elasticity of their facial skin was significantly improved, and the test product had the effect of tightening the skin.

[0175] It indicates that under the test conditions, the test product has a firming effect.

[0176] 3. At the 28-day follow-up, 100.00% of the subjects felt that their facial redness had improved after using the test product.

[0177] 100.00% of the subjects felt that facial skin tightness, itching or other skin discomfort was relieved; 100.00% of the subjects felt that their facial skin was comfortable and their skin condition was more stable; 100.00% of the subjects felt that the test product had a soothing effect.

[0178] This indicates that the test product has a soothing effect under the test conditions.

[0179] 4. At the 28-day follow-up, after using the test product, 100.00% of the subjects felt that the test product was suitable for sensitive skin.

[0180] It indicates that under the test conditions, the test product is suitable for sensitive skin.

[0181] 5. At the 28-day follow-up visit, 100.00% of the subjects felt that there was no irritation, allergy or other adverse reactions on their facial skin after using the test product, and felt that the test product was mild and non-irritating.

[0182] It indicates that under the test conditions, the test product has a mild and non-irritating effect.

[0183] Comparative Example 1

[0184] Compared with Example 1, this control example is a basic cream, which does not contain anti-wrinkle agents, mango (MANGIFERA INDICA) seed fat, bifida yeast fermentation product filtrate, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-5 and other ingredients, and correspondingly increases the amount of hydrolyzed hyaluronic acid, palmitoyl pentapeptide-4, sodium hyaluronate, and glycerol. The specific formula is shown in Tables 12 and 13 below.

[0185] Table 12 Basic facial cream formula

[0186]

[0187]

[0188] Table 13 Dosage ratio of plant extracts

[0189]

[0190] Test Example 2

[0191] 1. Basic Information

[0192] 1.1 There were 31 subjects in total, including 0 males and 31 females, aged 35 to 60 years old, with an average age of 50±7 years old, who met the voluntary inclusion criteria.

[0193] 1.2 Sample group: the anti-wrinkle and firming cream of Example 1.

[0194] 1.3 Control group: basic facial cream of control example 1.

[0195] 2. Instruments

[0196] Skin elasticity tester (ELM1201, Delfin), facial image analyzer (VISIA), Antera3D imager (HC1023Q, Delfin) balance (accuracy 0.01g).

[0197] 3. Testing environment

[0198] Test environment temperature: 20.0℃~22.0℃; humidity: 40%~60%, and dynamically monitored.

[0199] 4. Test methods

[0200] 4.1 At the first visit, the subjects were given a brief explanation of the trial and signed the informed consent form.

[0201] 4.2 The subjects participating in the trial were screened according to the test requirements, and 31 people entered the experimental phase.

[0202] 4.3 The subjects cleaned their faces on the day of the test and after balancing in the test environment for 20 minutes, the observed parts of the subjects were photographed by Antera 3D imager and facial image analyzer and measured by skin elasticity tester.

[0203] 4.4 The subjects visited again the next day for the hyaluronic acid injection.

[0204] 4.5 Three days after the injection of the water light project, visit again, clean the face on the test day, and after balancing in the test environment for 20 minutes, the observed parts of the subjects will be photographed by Antera 3D imager, facial image analyzer, and measured by skin elasticity tester.

[0205] 4.6 After the measurement is completed, the sample usage instructions are given and the samples are distributed. The control group uses the basic cream on the left face once in the morning and evening, 0.25g each time; the sample group uses the anti-wrinkle firming cream on the right face once in the morning and evening, 0.25g each time. The sample weight is confirmed at the beginning and end of the test.

[0206] 4.7 Use Antera 3D imager, facial image analyzer and skin elasticity tester to take photos and measurements at designated return visit time points.

[0207] 5. Results Analysis

[0208] 5.1 Principle of before and after comparison

[0209] Descriptive statistics were performed on the measured values ​​of each test area, including quantity, mean, and standard deviation.

[0210] Calculate the initial value of each test area and the measured values ​​at other time points, and statistically analyze the differences between the measured values ​​and the initial values ​​at different times.

[0211] If the test data is normally distributed, the test method is used for statistical analysis; if the test data is non-normally distributed, the rank sum test method is used for statistical analysis.

[0212] The statistical methods all adopted two-tailed test, and the test level was α=0.05.

[0213] 5.2 Principle of parallel control

[0214] Descriptive statistics were performed on the measured values ​​of each test area, including quantity, mean, and standard deviation.

[0215] The difference between the initial value of each test area and the measured value at other time points was calculated, and then this difference was used to statistically analyze the differences between the sample group and the control group at different time points.

[0216] If the test data is normally distributed, the test method is used for statistical analysis; if the test data is non-normally distributed, the rank sum test method is used for statistical analysis.

[0217] The statistical methods all adopted two-tailed test, and the test level was α=0.05.

[0218] 6. Result determination

[0219] 6.1 Anti-wrinkle effect

[0220] Positive result: Compared with before use, wrinkles (number, length, depth) and fine lines (number, length, depth) in the test area were significantly reduced after the use of the sample, and compared with the control group, wrinkles (number, length, depth) and fine lines (number, length, depth) in the test area were significantly reduced after the use of the sample, indicating that the sample has anti-wrinkle effect.

[0221] Negative result: Compared with before use, there is no significant difference in wrinkles (number, length, depth) and fine lines (number, length, depth) in the test area after the use of the sample, or compared with the control group, there is no significant difference in wrinkles (number, length, depth) and fine lines (number, length, depth) in the test area after the use of the sample, indicating that the sample has no anti-wrinkle effect.

[0222] 6.2 Firming effect

[0223] Positive result: Compared with before use, after using the sample, the skin elasticity coefficient R2 value of the test area was significantly increased, the skin firmness F4 value was significantly decreased, and the jaw line angle was significantly decreased. Compared with the control group, after using the sample, the skin elasticity coefficient R2 value of the test area was significantly increased, the skin firmness F4 value was significantly decreased, and the jaw line angle was significantly decreased, indicating that the sample has a firming effect.

[0224] Negative result: Compared with before use, there are no significant differences in the skin elasticity coefficient R2 value, skin firmness F4 value, and jaw line angle in the test area after the use of the sample, or compared with the control group, there are no significant differences in the skin elasticity coefficient R2 value, skin firmness F4 value, and jaw line angle in the test area after the use of the sample, indicating that the sample does not have a firming effect.

[0225] 7. Test conclusion

[0226] After the subjects had undergone cosmetic surgery (water light needle) surgery, the length of the subjects' crow's feet fine lines was significantly reduced after continuous use for 2 and 4 weeks, with a reduction rate of 12.02% and 23.15% respectively. After 4 weeks of use, the improvement rates of the sample group were 4 times and 3 times that of the control group respectively. After 4 weeks of use, the depth of the subjects' crow's feet wrinkles was significantly reduced, with a reduction rate of 13.96%, and compared with the control group, the improvement rate of the sample group was 8 times that of the control group. The length of crow's feet wrinkles was significantly reduced, with a reduction rate of 39.55%, and compared with the control group, the improvement rate of the sample group was The improvement rate was 20 times that of the control group; the depth of crow's feet fine lines was significantly reduced, with a reduction rate of 7.64%, and compared with the control group, the improvement rate of the sample group was twice that of the control group; the width of nasolabial folds was significantly reduced, with a reduction rate of 21.06%, and compared with the control group, the improvement rate of the sample group was 5 times that of the control group; the number of forehead wrinkles was significantly reduced, with a reduction rate of 42.06%, the length of forehead wrinkles was significantly reduced, with a reduction rate of 34.64%, the number of forehead fine lines was significantly reduced, with a reduction rate of 30.08%, and the length of forehead fine lines was significantly reduced, with a reduction rate of 30.44%. This shows that the sample can increase the anti-wrinkle effect of medical beauty surgery (water light needle) when used after medical beauty surgery, and has anti-wrinkle effect.

[0227] After the subjects used the samples for 2 and 4 weeks after medical aesthetic surgery (water light needle), the angle of the subjects' jaw line was significantly lowered compared with before use, with a reduction rate of 0.52% and 0.98% respectively. After 4 weeks of use, the improvement rates of the sample group were 1 and 1 times of the improvement rates of the control group respectively; after using the samples for 2 and 4 weeks, the R2 value of the subjects' skin elasticity coefficient was significantly improved, with an improvement rate of 18.93% and 39.62% respectively, and compared with the control group, the improvement rates of the sample group were 2 and 2 times of the improvement rates of the control group respectively; after using the samples for 2 and 4 weeks, the F4 value of the subjects' skin firmness was significantly lowered, with a reduction rate of 15.83% and 40.05% respectively. After 4 weeks of use, compared with the control group, the improvement rate of the sample group was 2 times of the improvement rate of the control group. This shows that the use of the samples after medical aesthetic surgery can increase the firming effect of medical aesthetic surgery (water light needle) and has a firming effect.

[0228] After the subjects used the samples for 2 and 4 weeks after medical aesthetic surgery (water light needle), the subjects' skin hemoglobin index was significantly reduced compared with before use, with a reduction rate of 4.94% and 7.66% respectively. After using the samples for 4 weeks, compared with the control group, the improvement rate of the sample group was twice that of the control group. This shows that the samples can effectively soothe the skin after medical aesthetic surgery (water light needle) and have a soothing effect after medical aesthetic surgery.

[0229] According to consumer use tests, 100% of the subjects experienced no irritation, redness, fever, itching, rash, acne and other adverse reactions after using the samples for 4 weeks.

[0230] According to consumer use tests, after using the sample for 4 weeks, 100% of the subjects were satisfied with the overall gentleness (non-irritating) of the sample, and 100% of the subjects were satisfied with the lack of eye irritation after use.

[0231] According to consumer usage tests, after using the samples for 4 weeks, 100% of the subjects were satisfied with the texture of the samples; 100% of the subjects were satisfied with the smell of the samples; 100% of the subjects were satisfied with the hydration of their faces after using the samples; 100% of the subjects were satisfied with the firming and anti-wrinkle effects after using the samples; 100% of the subjects were satisfied with the moisturizing effects after using the samples; 100% of the subjects were satisfied with the refreshment and lack of burden after using the samples; 100% of the subjects were satisfied with the soothing and relaxation of their faces without redness after using the samples.

[0232] The above is a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements and modifications without departing from the principle of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. An anti-wrinkle and firming facial cream, characterized in that: The composition comprises the following components by mass percentage: 4% to 20% of emollient, 2% to 9% of moisturizer, 0.5% to 3% of anti-wrinkle agent and the balance of solvent; The emollient includes a plant extract; The plant extract comprises the following raw materials: citrus fruit extract, burdock root extract, schizonepeta extract, Centella asiatica extract, fig fruit extract, apple fruit extract, kava leaf / root / stem extract, cocoa seed extract and mussel extract; The moisturizing agent comprises the following raw materials: glycerin, butylene glycol, betaine, sodium hyaluronate and trehalose; The anti-wrinkle agent includes hydroxypropyl tetrahydropyrantriol.

2. The anti-wrinkle and firming facial cream according to claim 1, characterized in that: The following components are also included by mass percentage: 0.02% to 0.08% of chelating agent, 0.5% to 3% of surfactant, 0.3% to 3% of antioxidant, 1% to 5% of emulsifier, 0.2% to 2% of emulsion stabilizer and 1% to 3% of thickener; The emulsion stabilization comprises at least one of palmitic acid, stearic acid, cetearyl alcohol, cetyl alcohol or sodium stearoyl glutamate; The emulsifier includes at least one of cetearyl glucoside, glyceryl stearate or PEG-75 stearate; The thickener includes sodium polyacrylate and / or dextrin; The antioxidant comprises at least one of tocopheryl acetate, tocopherol or p-hydroxyacetophenone; The surfactant includes ceteth-20 and / or steareth-20; The solvent includes water and / or 1,2-hexanediol; The chelating agent includes disodium EDTA.

3. The anti-wrinkle and firming facial cream according to claim 1, characterized in that: The invention comprises the following components by mass percentage: 12% to 18% of emollient, 2% to 6% of moisturizing agent, 0.5% to 3% of anti-wrinkle agent and the remainder of solvent; the moisturizing agent comprises 0.5% to 3% of glycerol, 0.2% to 1% of butylene glycol, 0.2% to 1% of betaine, 0.5% to 2% of sodium hyaluronate and 0.2% to 2% of trehalose.

4. The anti-wrinkle and firming facial cream according to claim 1, characterized in that: The mass ratio of the citrus fruit extract, burdock root extract, schizodactylum extract, Centella asiatica extract, fig fruit extract, apple fruit extract, kava leaf / root / stem extract, cocoa seed extract and mussel extract is 1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2:1-2.

5. The anti-wrinkle and firming facial cream according to claim 1, characterized in that: The emollient further comprises one or more of cyclopentasiloxane, mango seed butter, bifida fermentation product filtrate, ethylhexylglycerin, hydrogenated lecithin, hydrolyzed hyaluronic acid, madecassoside, panthenol, caprylic / capric triglyceride, ceramide, raffinose, cholesterol, folic acid, soluble collagen, dipeptide diaminobutyryl benzylamide diacetate, palmitoyl tripeptide-5, palmitoyl pentapeptide-4, sucrose stearate or superoxide dismutase.

6. The anti-wrinkle and firming facial cream according to claim 5, characterized in that: By mass percentage, 0.2% to 2% of cyclopentasiloxane, 0.1% to 1.2% of mango seed fat, 0.2% to 2% of bifida yeast fermentation product filtrate, 0.2% to 2% of ethylhexylglycerol, 0.2% to 2% of hydrogenated lecithin, 0.5% to 3% of hydrolyzed hyaluronic acid, 0.5% to 3% of madecassoside, 0.5% to 2% of panthenol, 0.2% to 2% of caprylic / capric triglyceride, 0.2% to 2% of ceramide, 0.2% to 2% of raffinose, 0.2% to 2% of cholesterol, 0.2% to 2% of folic acid, 0.2% to 2% of soluble collagen, 0.2% to 2% of dipeptide diaminobutyryl benzylamide diacetate, 0.2% to 2% of palmitoyl tripeptide-5, and 0.2% to 2% of palmitoyl pentapeptide-4 are added. 0.2%~2%, sucrose stearate 0.2%~2% or superoxide dismutase 0.5%~2%.

7. The method for preparing an anti-wrinkle and firming facial cream according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: adding an emollient, a moisturizing agent and an anti-wrinkle agent into a solvent, and stirring at 400 to 800 rpm for 30 to 50 minutes.

8. The anti-wrinkle and firming facial cream according to any one of claims 1 to 6, comprising use in reducing the number and volume of under-eye wrinkles.

9. The anti-wrinkle and firming cream according to any one of claims 1 to 6 is used for improving wrinkles or fine lines, improving skin elasticity and firmness, or reducing skin hemoglobin after medical aesthetics.

10. The use according to claim 9, characterized in that: The improving wrinkles or fine lines includes reducing the number, length or depth of wrinkles or fine lines, and the wrinkles or fine lines include crow's feet, nasolabial folds or forehead wrinkles.

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