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Micro-molecule polypeptide with function of removing free radicals

A technology of small molecular peptides and free radicals, applied in the field of peptides, can solve the problems of loss of active peptides, poor molecular weight uniformity of peptides, affecting the function of free radical scavenging, etc., and achieve the effects of simple operation, good free radical scavenging effect, and wide source of raw materials.

Inactive Publication Date: 2015-05-06
AESTHETIC TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the technical solutions disclosed in the prior art, the molecular weight uniformity of the polypeptide is poor. After the polypeptide is subjected to post-treatment such as filtration or ultrafiltration, a large amount of active polypeptide will be lost, which will affect its free radical scavenging function.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Small molecule peptides are prepared according to the following steps:

[0032] 1) Soak 100g of earthworms in 200ml of 0.5% methylcellulose solution. After 30min, clean the earthworms, soak them in 300ml of pure water, and cut them with a high-speed shearer at 6000rpm for 3min to obtain a homogenate. ;

[0033] 2) Add hydrochloric acid to the homogenate obtained in step 1) to make the final concentration 0.4%, then add 0.3 g of pepsin with a specific activity of 3500 U / g, and react at 37° C. for 3 hours to obtain a pepsin hydrolyzate;

[0034] 3) Adjust the pH value of the pepsin hydrolyzate obtained in step 2) to 6.8, add 0.5 g of neutral protease with a specific activity of 50,000 U / g, and react at 50° C. for 2 hours to obtain a neutral protein hydrolyzate;

[0035] 4) Keep the pH value of the neutral protein hydrolyzate obtained in step 3) unchanged, add 0.8 g of bromelain with a specific activity of 800,000 U / g, and react at 55°C for 5 hours to obtain the bromelain...

Embodiment 2

[0038] Small molecule peptides are prepared according to the following steps:

[0039] 1) Soak 100g of earthworms in 300ml of 1% methylcellulose solution. After 45min, clean the earthworms, soak them in 200ml of pure water, and cut them with a high-speed shearer at 8000rpm for 3min to obtain a homogenate. ;

[0040]2) Add hydrochloric acid to the homogenate obtained in step 1) to make the final concentration 0.2%, then add 0.4 g of pepsin with a specific activity of 2500 U / g, and react at 36° C. for 2 hours to obtain pepsin hydrolyzate;

[0041] 3) Adjust the pH value of the pepsin hydrolyzate obtained in step 2) to 6.7, add 0.4 g of neutral protease with a specific activity of 30,000 U / g, and react at 48° C. for 2 hours to obtain a neutral protein hydrolyzate;

[0042] 4) Keep the pH value of the neutral protein hydrolyzate obtained in step 3) unchanged, add 0.5 g of bromelain with a specific activity of 400,000 U / g, and react at 54°C for 3 hours to obtain a bromelain hydrol...

Embodiment 3

[0045] Small molecule peptides are prepared according to the following steps:

[0046] 1) Soak 100g of earthworms in 500ml of 2% methylcellulose solution, after 60min, clean the earthworms, soak them in 500ml of pure water, and cut them with a high-speed shearer at 6000rpm for 10min to obtain a homogenate ;

[0047] 2) Add hydrochloric acid to the homogenate obtained in step 1) to make the final concentration 0.4%, then add 0.5 g of pepsin with a specific activity of 4000 U / g, and react at 38° C. for 5 hours to obtain a pepsin hydrolyzate;

[0048] 3) Adjust the pH value of the pepsin hydrolyzate obtained in step 2) to 6.9, add 0.8 g of neutral protease with a specific activity of 60,000 U / g, and react at 52° C. for 3 hours to obtain a neutral protein hydrolyzate;

[0049] 4) Keep the pH value of the neutral protein hydrolyzate obtained in step 3) unchanged, add 1.5 g of bromelain with a specific activity of 800,000 U / g, and react at 56°C for 5 hours to obtain a bromelain hyd...

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Abstract

The invention relates to a micro-molecule polypeptide with a function of removing free radicals. In the biological field related to removal of free radicals, earthworm is a novel high-protein source and is high in content of protein and rich in types of amino acids; an earthworm proteinase product prepared by autolysis of the earthworm or degradation of exogenous proteases has an excellent function of removing free radicals. The micro-molecule polypeptide is extracted from the earthworm and is prepared by the following steps: dispersing the earthworm into slurry, carrying out enzymolysis on the slurry by using different hydrolytic enzymes, carrying out suction filtration and ultrafiltration on enzymatic hydrolysate, concentrating and drying to obtain the micro-molecule polypeptide. The micromolecule polypeptide is low in molecular weight and low in sensitization; the total content of polypeptide with the molecular weight being smaller than 1000Da is over 90%; by virtue of detection of the capability of removing DPPH, the micro-molecule polypeptide has the excellent function of removing the free radicals.

Description

technical field [0001] The invention relates to the field of polypeptides, in particular to a small molecule polypeptide with the function of scavenging free radicals. Background technique [0002] Oxidants that can reduce free radicals to non-free radicals are called free radical scavengers. Free radical scavengers refer to the general term for substances that delay, inhibit and block ROS (reactive oxygen species) / OFR (oxygen free radicals) oxidative damage, and are body protection agents that can combine with OFR and remove it. Therefore, it plays an important role in the normal processes of the body and in protecting cells and tissues from oxidative damage. At present, free radical scavengers of enzymes and polypeptides have attracted more and more attention. [0003] Modern medical research shows that earthworms have various physiological functions such as antithrombotic, antiasthma, skin repair, antibacterial, and antioxidation. Usually, the extracts obtained by decoc...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 陈光李仕林丁爽
Owner AESTHETIC TECH BEIJING
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