Preparation method of hypolycemic essence by compounding bifidobacterium lactis and traditional Chinese medicine extracts through fermentation

A technology of bifidobacterium lactis and its extracts, which is applied in the field of preparation of hypoglycemic essence using bifidobacterium lactis fermented and compounded traditional Chinese medicine extracts, can solve the problems of undisclosed hypoglycemic products and achieve the effect of improving metabolism

Inactive Publication Date: 2022-01-21
河北一然生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the prior art has not disclosed any hypoglycemic products related to Bifidobacterium lactis

Method used

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  • Preparation method of hypolycemic essence by compounding bifidobacterium lactis and traditional Chinese medicine extracts through fermentation
  • Preparation method of hypolycemic essence by compounding bifidobacterium lactis and traditional Chinese medicine extracts through fermentation
  • Preparation method of hypolycemic essence by compounding bifidobacterium lactis and traditional Chinese medicine extracts through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The steps of this embodiment are as follows:

[0035] 1. Weigh 1Kg of mulberry leaves, 1Kg of salvia miltiorrhiza, 1Kg of rehmannia glutinosa, and 1Kg of corn silk.

[0036] 2. Add 1Kg dandelion to 15L pure water for cooking for 45 minutes, filter and retain the filtrate, add 10L pure water to the dregs and cook for 30 minutes, filter again to obtain the filtrate. The two filtrates are combined for cooking and concentration to obtain a concentrated liquid of mulberry leaves.

[0037] 3. Treat Danshen, Rehmannia glutinosa, and corn silk according to the same method as above, and obtain the water-extracted concentrated liquid of Danshen, the concentrated liquid of rehmannia glutinosa, and the concentrated liquid of corn silk.

[0038] 4. The concentrated cooking liquid of four kinds of traditional Chinese medicines is freeze-dried in a freeze-drying machine. The freeze-drying machine is a Tofflon freeze-drying machine. The freeze-drying time is 24 hours, and then the fre...

Embodiment 2

[0063] The difference between this example and Example 1 is that the fifth step in this example is: according to the freeze-dried powder of mulberry leaf extract 20%, the freeze-dried powder of Salvia miltiorrhiza extract 35%, and the freeze-dried powder of Rehmannia glutinosa extract 25%. and 20% of corn silk extract freeze-dried powder were mixed to obtain 10 g of mixed medicinal powder.

[0064] The rest of the experimental steps and conditions refer to Example 1. After the fermentation is completed, the supernatant is taken for later use, and the in vitro hypoglycemic activity of the supernatant is tested according to the method of Example 1.

[0065] Finally, by calculating the inhibition rate, the essence prepared in this example has an inhibition rate of 64% for α-glucosidase.

Embodiment 3

[0067] The difference between this example and Example 1 is that the fifth step in this example is: according to 20% lyophilized powder of mulberry leaf extract, 40% lyophilized powder of Salvia miltiorrhiza extract, and 30% lyophilized powder of rehmannia root extract and 10% of corn silk extract freeze-dried powder were mixed to obtain 10 g of mixed medicinal powder.

[0068] The rest of the experimental steps and conditions refer to Example 1. After the fermentation is completed, the supernatant is taken for later use, and the in vitro hypoglycemic activity of the supernatant is tested according to the method of Example 1.

[0069] Finally, by calculating the inhibition rate, the essence prepared in this example has an inhibition rate of α-glucosidase of 47.8%.

[0070] It can be seen from the above examples 1-3 that the inhibitory rate of the essence prepared in the three examples to α-glucosidase is between 47% and 64%, which shows that the fermentation supernatant prepar...

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Abstract

The invention relates to a preparation method of hypolycemic essence by compounding bifidobacterium lactis and traditional Chinese medicine extracts through fermentation. The preparation method comprises the following steps: S1, preparing the traditional Chinese medicine extracts from traditional Chinese medicines including salvia miltiorrhiza, folium mori, radix rehmanniae and corn stigma; respectively carrying out water extraction and concentration on the traditional Chinese medicines and preparing medicine powder; and S2, preparing the essence through mixed fermentation: preparing a fermentation culture medium, adding a certain amount of mixed medicine powder based on total amount of the fermentation culture medium, inoculating bifidobacterium lactis BAL531 ( preservation number of CGMCC No.17329) for mixed fermentation, centrifuging after fermentation and taking supernatant to prepare the hypolycemic traditional Chinese medicine fermented essence. The fermentation culture medium comprises the following components: 16-24 g of glucose, 55-65 g of milk powder, 3-6 g of whey protein, 3-6 g of yeast extract powder, 1.5-2.5 g of dipotassium phosphate, 1.5-2.5 g of anhydrous sodium acetate, 0.54- 0.62 g of magnesium sulfate, 0.45-0.55 g of L-cysteine hydrochloride and 0.8- 1.2 g of Tween in per 1L of culture solution. The pH value of the culture solution is adjusted to 6.8-7.2, and the inoculation amount of BAL531 is 3+ / -0.5%. According to the essence of the invention, traditional Chinese medicines with good hypoglycemic activity and bifidobacterium lactis with a special metabolic regulation function are combined, so that the essence has up to 47.8-64% of inhibition rate on alpha-glucosidase, and remarkable hypoglycemic effect.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine fermented medicines, in particular to a method for preparing a blood sugar-lowering essence by using bifidobacterium lactis to ferment compound Chinese medicine extracts. Background technique [0002] Diabetes is a syndrome of metabolic abnormalities caused by the interaction of genetic and environmental factors. Long-term diabetes can cause chronic complications of multiple system organs, leading to dysfunction and failure, and become the main cause of disability and death. Affected by eating habits and rhythm of life, modern people have gradually increased the prevalence of hyperglycemia. Hyperglycemia is extremely harmful to the human body and can cause some acute or chronic complications; acute complications such as diabetic ketosis, ketoacidosis or Lead to hyperglycemia and hyperosmolar state; chronic complications such as fundus lesions, diabetic foot, etc. In addition,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A61P3/10A61K35/745
CPCA61K36/537A61K36/605A61K36/804A61K36/899A61K35/745A61P3/10A61K2236/19A61K2236/331A61K2236/39A61K2300/00
Inventor 杨玲齐世华赵林森智朋飞霍文敏郭红敏霍世和申朋仵红岩贾洪利
Owner 河北一然生物科技股份有限公司
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