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Glycosyl grain protein-selenium compound and production thereof

A protein and selenium technology, applied in food preparation, food science, climate change adaptation and other directions, can solve the problem of no uniform standard for selenium demand, and achieve the effect of improving use safety, high affinity and low production cost

Inactive Publication Date: 2005-12-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] There is no uniform standard for the human body's demand for selenium

Method used

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  • Glycosyl grain protein-selenium compound and production thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0027]The preparation of glycosylated cereal protein-selenium complex is carried out in two steps. The first step is to add monosaccharide or disaccharide to the alkaline cereal protein suspension, and make the cereal protein and monosaccharide or disaccharide under high temperature and high pressure conditions. Sugar undergoes Maillard reaction, using side chain amino groups such as glutamine to react with aldehyde groups of monosaccharides or polysaccharides to attach monosaccharide or polysaccharide units to protein molecules, thereby increasing the hydroxyl density of protein molecules. The Maillard reaction between protein and reducing sugar can proceed spontaneously at low temperature, but the reaction time usually takes several days; the reaction can also be carried out at a higher temperature such as 120°C (Brands C.M.J., van BoekelM.A.J.S., Reactions of Monosaccharides during Heating of Sugar-Casein Systems: Building of a Reaction Network Model. J. Agric. Food Chem., 2...

Embodiment 1

[0048] The preparation method of preparing glycosylated cereal protein-selenium element compound with gluten, glucose and selenic acid as raw materials comprises the following steps:

[0049] 1) Take by weighing 20g gluten powder and place it in a beaker, make a 3% suspension with deionized water, add saturated NaOH solution while electromagnetic stirring, adjust the pH value of the suspension to 7, continue stirring at 50°C for 30min, After cooling, add 4g of glucose and stir until the glucose dissolves;

[0050] 2) Put the suspension in a pressure cooker, carry out the glycosylation reaction at 120°C and 0.11MPa for 10 minutes, after cooling, adjust the pH to 5 with saturated acetic acid solution, place it in a dialysis bag and dialyze it with deionized water for 10 hours to remove inorganic Salt;

[0051] 3) Make a 3% suspension of the dialyzed glycosylated cereal protein with deionized water, add 0.6g of selenic acid, stir until the selenic acid is dissolved, react at 5°C...

Embodiment 2

[0053] A preparation method for preparing a glycosylated cereal protein-selenium complex using gluten, mannose, and selenous acid as raw materials comprises the following steps:

[0054] 1) Take by weighing 10g gluten powder and place it in a beaker, make a 15% suspension with deionized water, add saturated NaHCO while electromagnetic stirring 3 Solution, adjust the pH value of the suspension to 12, continue stirring at 90°C for 10 minutes, add 0.5g mannose after cooling, and stir until the mannose dissolves;

[0055] 2) Put the suspension in a pressure cooker, carry out glycosylation reaction at 126°C and 0.15MPa for 2 minutes, after cooling, adjust the pH to 7 with saturated hydrochloric acid solution, put it in a dialysis bag and dialyze it with deionized water for 20 hours to remove inorganic Salt;

[0056] 3) Make a 15% suspension of the dialyzed glycosylated cereal protein with deionized water, add 0.08g of selenous acid, stir until the selenous acid is dissolved, react...

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Abstract

A glycosylated grain's protein-Se compound used as the Se-supplementing additive of food is prepared through the reacting between the active chemical radical of glycosylated grain's protein and selenic acid or seleninic acid, centrifugal separating, and filtering or spray drying to obtain the powdered product.

Description

technical field [0001] The invention relates to a glycosylated cereal protein-selenium complex and a preparation method thereof. Background technique [0002] Selenium is one of the essential trace elements for the human body, and its contribution to the physiological functions of the human body is mainly manifested in the following aspects: [0003] 1) Selenium is an important component of glutathione peroxidase (GSH-Px) and selenoenzyme-phospholipid hydroperoxide glutathione peroxide (PHGPX). GSH-Px is widely present in red blood cells, liver, lung, heart, kidney, brain and other tissues of mammals. By catalyzing the conversion of reduced glutathione (GSH) to oxidized glutathione (GSSG), it eliminates harmful processes in the body. oxides, which protect biofilms from damage caused by peroxides. PHGPX can protect biological membranes by inhibiting membrane phospholipid peroxidation. Under normal circumstances, the human body can remove free radicals and lipid perhydrogen...

Claims

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Application Information

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IPC IPC(8): A23L33/16
CPCY02A40/90
Inventor 宋义虎郑强叶瑛
Owner ZHEJIANG UNIV
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