Method for preparing antioxidative peptide by utilizing beer grain proteins

A technology of antioxidant polypeptide and brewer's grains, which is applied in the field of resource utilization of brewer's grains, can solve the problems of undiscovered patent documents, etc., achieve good antioxidant capacity, reduce high costs, and increase economic benefits

Inactive Publication Date: 2020-01-10
天津实发中科百奥工业生物技术有限公司 +2
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  • Application Information

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[0006] Through the search of published patent documents, no published

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  • Method for preparing antioxidative peptide by utilizing beer grain proteins
  • Method for preparing antioxidative peptide by utilizing beer grain proteins
  • Method for preparing antioxidative peptide by utilizing beer grain proteins

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[0031] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0032] A method for preparing antioxidant polypeptides from brewer's grain protein, the innovation of which is that the method includes the following steps:

[0033] 1) Cellulase enzymatic hydrolysis of brewer's grains: put wet brewer's grains in a drying box, and blow dry them at 60°C, then pulverize them with a pulverizer, and pass through an 80-mesh sieve; sieve the dry brewer's grains Dissolve in water with water at a material-to-liquid ratio of 1:10, and adjust the pH value of the solution to 5-6 with dilute hydrochloric acid, add cellulase with a mass fraction of 2.0%-2.5% to the solution, and heat it at 50-55°C Enzymolysis for 4 to 5 hours, inactivate the beer lees feed liquid after cellulase enzymolysis in a water bath at 85°C for 15...

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Abstract

The invention relates to a method for preparing antioxidative peptide by utilizing beer grain proteins. The method comprises the following steps: 1), performing enzymatic hydrolysis on beer grains viacellulase; 2), performing enzymatic hydrolysis on beer grains via proteases; 3), determining the hydrolysis degree of beer grain proteins; 4), determining the yield of beer grain polypeptide; 5), performing separation and purification on the beer grain polypeptide; and 6), extracting water-insoluble dietary fibers in the beer grains. The method disclosed by the invention is scientific and reasonable in design; by adopting the method, by-products in beer industries can be utilized and extracted, so that waste products are reduced, utilization ratios of resources are increased, the economic benefit is improved, the production cost is reduced, and the oxidation resistance of the obtained polypeptide is high.

Description

technical field [0001] The invention belongs to the field of resource utilization of brewer's grains, relates to a method for comprehensive utilization of brewer's grains, in particular to a method for preparing antioxidant polypeptides by using brewer's grains protein. Background technique [0002] Brewer's grains, also known as wheat grains and malt grains, are the by-products of my country's brewing industry. The output is huge and the price is low. The protein content is significantly higher than that of other distiller's grains (potatoes, corn, sorghum, barley, etc.), and is often used as raw material for microbial fermentation and roughage for ruminants, with little economic benefit. Brewer's grains have high water content and large output. During the peak production season, the brewer's grains are often pulled away or discharged directly due to the lack of proper treatment technology by the manufacturer, which not only causes waste of resources, but also easily pollute...

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Application Information

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IPC IPC(8): A23J3/14A23J3/34A23L33/18A23L33/22C12P21/06C07K1/34
CPCA23J3/14A23J3/346A23L33/18A23L33/22C12P21/06C07K1/34A23V2002/00A23V2200/30A23V2250/55A23V2300/14
Inventor 曲亮璠郑雅元曹琳琳薛薇许勤虎沙印发
Owner 天津实发中科百奥工业生物技术有限公司
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